Restriction map for
pF1KB0904
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII BbrPI
BbsI BbvCI BcgI BfrI BglII
BpmI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI HindIII HpaI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MroI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 109 CGCG CG^CG
AcyI 88 GGCGCC G[GA]^CG[CT]C
AlwNI 581 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 418 GTGCTC G[AT]GC[AT]^C
AvaI 47 CCCGGG C^[CT]CG[GA]G
AvrII 369 CCTAGG C^CTAGG
BamHI 212 GGATCC G^GATCC
BanI 88 GGCGCC G^G[CT][GA]CC
BbeI 88 GGCGCC GGCGC^C
BciVI 478 GTATCC GTATCC(6/5)
BclI 458 TGATCA T^GATCA
BlnI 369 CCTAGG C^CTAGG
BlpI 558 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 558 GCTGAGC GC^T[ACGT]AGC
BsaHI 88 GGCGCC G[GA]^CG[CT]C
BseRI 270 CTCCTC GAGGAG(10/8)
BsiEI 788 CGATCG CG[GA][CT]^CG
BsiHKAI 418 GTGCTC G[AT]GC[AT]^C
BsmAI 327 GTCTC GTCTC(1/5)
BsmFI 27 GGGAC GGGAC(10/14)
BsoBI 47 CCCGGG C^[CT]CG[GA]G
BsrFI 85 GCCGGC [GA]^CCGG[CT]
BsrI 225 CCAGT ACTGG(1/-1)
BstUI 109 CGCG CG^CG
BstXI 596 CCAAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 212 GGATCC [GA]^GATC[CT]
CelII 558 GCTGAGC GC^T[ACGT]AGC
Cfr10I 85 GCCGGC [GA]^CCGG[CT]
FbaI 458 TGATCA T^GATCA
Hin1I 88 GGCGCC G[GA]^CG[CT]C
HincII 409 GTCAAC GT[CT]^[GA]AC
HindII 409 GTCAAC GT[CT]^[GA]AC
KasI 88 GGCGCC G^GCGCC
MaeIII 872 GTGAC ^GT[ACGT]AC
MflI 212 GGATCC [GA]^GATC[CT]
MvnI 109 CGCG CG^CG
NaeI 85 GCCGGC GCC^GGC
NarI 88 GGCGCC GG^CGCC
NgoMIV 85 GCCGGC G^CCGGC
NspI 356 GCATGT [GA]CATG^[CT]
PflMI 630 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 788 CGATCG CGAT^CG
SfaNI 708 GATGC GCATC(5/9)
SfiI 618 GGCCCAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 88 GGCGCC GGC^GCC
SmaI 47 CCCGGG CCC^GGG
SmlI 421 CTCAAG C^T[CT][GA]AG
Tsp45I 872 GTGAC ^GT[GC]AC
TspRI 722 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 630 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 212 GGATCC [GA]^GATC[CT]
XmaCI 47 CCCGGG C^CCGGG
XmaI 47 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BalI 434 TGGCCA TGG^CCA
545 TGGCCA
BfaI 353 CTAG C^TAG
370 CTAG
BglI 36 GCCCGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
619 GCCCAGCGGGC
BsgI 172 GTGCAG GTGCAG(16/14)
676 GTGCAG
DraII 43 GGGCCCC [GA]G^G[ACGT]CC[CT]
197 AGGGCCT
DsaI 92 CCGTGG C^C[GA][CT]GG
125 CCGTGG
EcoNI 189 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
879 CCTCATCAAGG
EcoO109I 43 GGGCCCC [GA]G^G[ACGT]CC[CT]
197 AGGGCCT
HaeII 88 GGCGCC [GA]GCGC^[CT]
365 AGCGCC
MaeI 353 CTAG C^TAG
370 CTAG
MboII 149 TCTTC GAAGA(8/7)
152 TCTTC
MluNI 434 TGGCCA TGG^CCA
545 TGGCCA
MscI 434 TGGCCA TGG^CCA
545 TGGCCA
NlaIII 54 CATG CATG^
357 CATG
Tsp509I 632 AATT ^AATT
672 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TfiI TseI TthHB8I