Restriction map for
pF1KB6168
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstPI ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 782 TCCGGA T^CCGGA
AocI 451 CCTGAGG CC^T[ACGT]AGG
BalI 1727 TGGCCA TGG^CCA
BamHI 1203 GGATCC G^GATCC
BbsI 624 GAAGAC GAAGAC(2/6)
BfaI 130 CTAG C^TAG
BpuAI 624 GAAGAC GAAGAC(2/6)
BseAI 782 TCCGGA T^CCGGA
BsiEI 41 CGGCCG CG[GA][CT]^CG
BspEI 782 TCCGGA T^CCGGA
Bst1107I 723 GTATAC GTA^TAC
BstXI 986 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 723 GTATAC GTA^TAC
Bsu36I 451 CCTGAGG CC^T[ACGT]AGG
EagI 41 CGGCCG C^GGCCG
EarI 1303 CTCTTC CTCTTC(1/4)
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
Eco81I 451 CCTGAGG CC^T[ACGT]AGG
HincII 1108 GTCGAC GT[CT]^[GA]AC
HindII 1108 GTCGAC GT[CT]^[GA]AC
Ksp632I 1303 CTCTTC CTCTTC(1/4)
MaeI 130 CTAG C^TAG
MaeII 335 ACGT A^CGT
MluNI 1727 TGGCCA TGG^CCA
MroI 782 TCCGGA T^CCGGA
MscI 1727 TGGCCA TGG^CCA
MspA1I 1138 CAGCTG C[AC]G^C[GT]G
NcoI 296 CCATGG C^CATGG
NspI 1127 ACATGC [GA]CATG^[CT]
PflMI 1220 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1138 CAGCTG CAG^CTG
SalI 1108 GTCGAC G^TCGAC
SapI 1302 GCTCTTC GCTCTTC(1/4)
SexAI 1537 ACCTGGT A^CC[AT]GGT
SgrAI 104 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1064 CCCGGG CCC^GGG
SspI 1439 AATATT AAT^ATT
StuI 1802 AGGCCT AGG^CCT
TfiI 344 GATTC G^A[AT]TC
Van91I 1220 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1064 CCCGGG C^CCGGG
XmaI 1064 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 723 GTATAC GT^[AC][GT]AC
1108 GTCGAC
AvaI 658 CCCGAG C^[CT]CG[GA]G
1064 CCCGGG
BanI 36 GGCACC G^G[CT][GA]CC
1480 GGTGCC
BbvCI 1572 CCTCAGC CCTCAGC(-5/-2)
1198 GCTGAGG
BglII 206 AGATCT A^GATCT
558 AGATCT
BlpI 666 GCTGAGC GC^T[ACGT]AGC
1770 GCTGAGC
Bpu1102I 666 GCTGAGC GC^T[ACGT]AGC
1770 GCTGAGC
BsaWI 782 TCCGGA [AT]^CCGG[AT]
1007 ACCGGA
BsmBI 937 CGTCTC CGTCTC(1/5)
1795 CGTCTC
BsoBI 658 CCCGAG C^[CT]CG[GA]G
1064 CCCGGG
CelII 666 GCTGAGC GC^T[ACGT]AGC
1770 GCTGAGC
DraII 62 GGGTCCC [GA]G^G[ACGT]CC[CT]
125 AGGTCCT
EcoO109I 62 GGGTCCC [GA]G^G[ACGT]CC[CT]
125 AGGTCCT
HaeII 152 GGCGCT [GA]GCGC^[CT]
1555 AGCGCC
HgaI 214 GACGC GACGC(5/10)
1059 GCGTC
MslI 1117 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1752 CACTATGGTG
NaeI 146 GCCGGC GCC^GGC
1733 GCCGGC
NgoMIV 146 GCCGGC G^CCGGC
1733 GCCGGC
PpuMI 62 GGGTCCC [GA]G^G[AT]CC[CT]
125 AGGTCCT
SfaNI 852 GATGC GCATC(5/9)
1214 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII