################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BlnI BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 116 GGTACC G^GTACC AccII 524 CGCG CG^CG AcyI 246 GACGCC G[GA]^CG[CT]C AocI 567 CCTCAGG CC^T[ACGT]AGG Asp718I 116 GGTACC G^GTACC BamHI 497 GGATCC G^GATCC BanI 116 GGTACC G^G[CT][GA]CC BcgI 344 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 476 AGATCT A^GATCT BlpI 421 GCTGAGC GC^T[ACGT]AGC Bpu1102I 421 GCTGAGC GC^T[ACGT]AGC BsaHI 246 GACGCC G[GA]^CG[CT]C BsiEI 122 CGATCG CG[GA][CT]^CG BsmAI 653 GTCTC GTCTC(1/5) BssHII 523 GCGCGC G^CGCGC BstUI 524 CGCG CG^CG Bsu36I 567 CCTCAGG CC^T[ACGT]AGG CelII 421 GCTGAGC GC^T[ACGT]AGC DraIII 532 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 246 GACGCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 567 CCTCAGG CC^T[ACGT]AGG EcoNI 391 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 293 CCATGG C^C[AT][AT]GG HaeII 425 AGCGCC [GA]GCGC^[CT] Hin1I 246 GACGCC G[GA]^CG[CT]C HinfI 651 GAGTC G^A[ACGT]TC HphI 317 TCACC GGTGA(8/7) KpnI 116 GGTACC GGTAC^C MboII 479 TCTTC GAAGA(8/7) MvnI 524 CGCG CG^CG NcoI 293 CCATGG C^CATGG PflMI 531 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 651 GAGTC GAGTC(4/5) PshAI 646 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 122 CGATCG CGAT^CG ScaI 491 AGTACT AGT^ACT StyI 293 CCATGG C^C[AT][AT]GG Van91I 531 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 668 CCACAATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 498 GATCC GGATC(4/5) 497 GGATC ApaI 67 GGGCCC GGGCC^C 541 GGGCCC AspHI 307 GTGCTC G[AT]GC[AT]^C 370 GAGCAC BsaAI 192 CACGTA [CT]AC^GT[GA] 304 TACGTG BsgI 264 CTGCAC GTGCAG(16/14) 439 GTGCAG BsiHKAI 307 GTGCTC G[AT]GC[AT]^C 370 GAGCAC BsrDI 186 CATTGC GCAATG(2/0) 99 GCAATG BsrI 494 ACTGG ACTGG(1/-1) 647 ACTGG BstF5I 564 CATCC GGATG(2/0) 276 GGATG BstYI 476 AGATCT [GA]^GATC[CT] 497 GGATCC DsaI 128 CCGTGG C^C[GA][CT]GG 293 CCATGG FokI 564 CATCC GGATG(9/13) 276 GGATG HgaI 246 GACGC GACGC(5/10) 510 GCGTC MflI 476 AGATCT [GA]^GATC[CT] 497 GGATCC NlaIII 207 CATG CATG^ 294 CATG PpuMI 457 GGGTCCT [GA]G^G[AT]CC[CT] 617 AGGACCT PstI 51 CTGCAG CTGCA^G 238 CTGCAG Tsp45I 316 GTCAC ^GT[GC]AC 644 GTGAC XhoII 476 AGATCT [GA]^GATC[CT] 497 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsoBI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfcI SmaI TseI TspRI XmaCI XmaI