################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI BstYI CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HaeIII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 370 GTATAC GT^[AC][GT]AC AciI 119 CCGC CCGC(-3/-1) AcsI 167 AAATTC [GA]^AATT[CT] AlwI 450 GATCC GGATC(4/5) AocI 615 CCTGAGG CC^T[ACGT]AGG ApoI 167 AAATTC [GA]^AATT[CT] Asp700I 3 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 28 GGTCC G^G[AT]CC AviII 584 TGCGCA TGC^GCA BanII 620 GGGCTC G[GA]GC[CT]^C BmyI 620 GGGCTC G[AGT]GC[ACT]^C BpmI 385 CTCCAG CTGGAG(16/14) BseRI 322 GAGGAG GAGGAG(10/8) BsgI 427 GTGCAG GTGCAG(16/14) Bsp1286I 620 GGGCTC G[AGT]GC[ACT]^C Bst1107I 370 GTATAC GTA^TAC BstZ17I 370 GTATAC GTA^TAC Bsu36I 615 CCTGAGG CC^T[ACGT]AGG Cfr13I 28 GGTCC G^G[ACGT]CC DpnII 450 GATC ^GATC Eco81I 615 CCTGAGG CC^T[ACGT]AGG EcoNI 198 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 584 TGCGCA TGC^GCA HaeII 19 AGCGCC [GA]GCGC^[CT] MboI 450 GATC ^GATC MseI 593 TTAA T^TAA NdeII 450 GATC ^GATC SapI 622 GCTCTTC GCTCTTC(1/4) Sau3AI 450 GATC ^GATC Sau96I 28 GGTCC G^G[ACGT]CC TaqI 182 TCGA T^CGA Tru9I 593 TTAA T^TAA TthHB8I 182 TCGA T^CGA XbaI 480 TCTAGA T^CTAGA XmnI 3 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 211 ACATGT A^C[GA][CT]GT 227 ACATGT AlwNI 454 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 550 CAGTGGCTG BfaI 376 CTAG C^TAG 481 CTAG BsiYI 198 CCTGAGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 611 CCAGCCTGAGG BslI 198 CCTGAGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 611 CCAGCCTGAGG BsmAI 208 GAGAC GTCTC(1/5) 325 GAGAC BspLU11I 211 ACATGT A^CATGT 227 ACATGT BstF5I 177 CATCC GGATG(2/0) 298 GGATG CfoI 20 GCGC GCG^C 585 GCGC EarI 144 CTCTTC CTCTTC(1/4) 623 CTCTTC FokI 177 CATCC GGATG(9/13) 298 GGATG HhaI 20 GCGC GCG^C 585 GCGC HinP1I 20 GCGC G^CGC 585 GCGC HphI 336 GGTGA GGTGA(8/7) 140 TCACC Ksp632I 144 CTCTTC CTCTTC(1/4) 623 CTCTTC MaeI 376 CTAG C^TAG 481 CTAG MaeIII 215 GTAAC ^GT[ACGT]AC 224 GTAAC MwoI 73 GCCTTCCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 525 GCTGACGAAGC NspI 211 ACATGT [GA]CATG^[CT] 227 ACATGT Tsp509I 156 AATT ^AATT 168 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AfaI AluI BbvI BsaJI BsrI BssKI BstNI Cac8I DdeI EcoRII Fnu4HI HinfI ItaI MboII MnlI MvaI NlaIII PstI RsaI ScrFI SfaNI SfcI TfiI TseI TspRI