################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI Hin1I HindIII HpaI KasI KspI MaeII MaeIII MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 101 GGTACC G^GTACC AccI 367 GTAGAC GT^[AC][GT]AC AfaI 102 GTAC GT^AC AluI 11 AGCT AG^CT AlwNI 317 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 396 CCTTAGG CC^T[ACGT]AGG Asp700I 172 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 101 GGTACC G^GTACC AvaII 160 GGACC G^G[AT]CC BanI 101 GGTACC G^G[CT][GA]CC BbvI 116 GCTGC GCAGC(8/12) BcgI 372 CGAAAGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 364 CTAG C^TAG BsaBI 314 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 401 GGTCTC GGTCTC(1/5) BsmAI 402 GTCTC GTCTC(1/5) BstBI 169 TTCGAA TT^CGAA BstF5I 51 CATCC GGATG(2/0) Bsu36I 396 CCTTAGG CC^T[ACGT]AGG CfoI 114 GCGC GCG^C Cfr13I 160 GGACC G^G[ACGT]CC EarI 457 GAAGAG CTCTTC(1/4) Eco81I 396 CCTTAGG CC^T[ACGT]AGG Fnu4HI 116 GCTGC GC^[ACGT]GC FokI 51 CATCC GGATG(9/13) HhaI 114 GCGC GCG^C HinP1I 114 GCGC G^CGC HincII 443 GTCAAC GT[CT]^[GA]AC HindII 443 GTCAAC GT[CT]^[GA]AC HphI 184 GGTGA GGTGA(8/7) ItaI 116 GCTGC GC^[ACGT]GC KpnI 101 GGTACC GGTAC^C Ksp632I 457 GAAGAG CTCTTC(1/4) MaeI 364 CTAG C^TAG MamI 314 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NspV 169 TTCGAA TT^CGAA PflMI 432 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 436 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 102 GTAC GT^AC Sau96I 160 GGACC G^G[ACGT]CC SfuI 169 TTCGAA TT^CGAA SpeI 363 ACTAGT A^CTAGT TaqI 170 TCGA T^CGA TseI 116 GCTGC G^C[AT]GC Tsp509I 133 AATT ^AATT TthHB8I 170 TCGA T^CGA Van91I 432 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 172 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### BstYI 197 AGATCC [GA]^GATC[CT] 319 GGATCT HapII 299 CCGG C^CGG 305 CCGG HpaII 299 CCGG C^CGG 305 CCGG MflI 197 AGATCC [GA]^GATC[CT] 319 GGATCT MspI 299 CCGG C^CGG 305 CCGG NlaIII 388 CATG CATG^ 448 CATG SfaNI 3 GATGC GCATC(5/9) 379 GATGC XhoII 197 AGATCC [GA]^GATC[CT] 319 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AlwI BsiYI BslI BssKI BstNI DdeI DpnII EcoRII HinfI MboI MboII MnlI MvaI NdeII NlaIV Sau3AI ScrFI TfiI