##################################
# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HaeIII HgaI Hin1I
HindIII HpaI KasI KspI MaeII
MaeIII MfeI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tsp45I TspRI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 101 GGTACC G^GTACC
AccI 367 GTAGAC GT^[AC][GT]AC
AfaI 102 GTAC GT^AC
AluI 11 AGCT AG^CT
AlwNI 317 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 396 CCTTAGG CC^T[ACGT]AGG
Asp700I 172 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 101 GGTACC G^GTACC
AvaII 160 GGACC G^G[AT]CC
BanI 101 GGTACC G^G[CT][GA]CC
BbvI 116 GCTGC GCAGC(8/12)
BcgI 372 CGAAAGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 364 CTAG C^TAG
BsaBI 314 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 401 GGTCTC GGTCTC(1/5)
BsmAI 402 GTCTC GTCTC(1/5)
BstBI 169 TTCGAA TT^CGAA
BstF5I 51 CATCC GGATG(2/0)
Bsu36I 396 CCTTAGG CC^T[ACGT]AGG
CfoI 114 GCGC GCG^C
Cfr13I 160 GGACC G^G[ACGT]CC
EarI 457 GAAGAG CTCTTC(1/4)
Eco81I 396 CCTTAGG CC^T[ACGT]AGG
Fnu4HI 116 GCTGC GC^[ACGT]GC
FokI 51 CATCC GGATG(9/13)
HhaI 114 GCGC GCG^C
HinP1I 114 GCGC G^CGC
HincII 443 GTCAAC GT[CT]^[GA]AC
HindII 443 GTCAAC GT[CT]^[GA]AC
HphI 184 GGTGA GGTGA(8/7)
ItaI 116 GCTGC GC^[ACGT]GC
KpnI 101 GGTACC GGTAC^C
Ksp632I 457 GAAGAG CTCTTC(1/4)
MaeI 364 CTAG C^TAG
MamI 314 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 169 TTCGAA TT^CGAA
PflMI 432 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 436 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 102 GTAC GT^AC
Sau96I 160 GGACC G^G[ACGT]CC
SfuI 169 TTCGAA TT^CGAA
SpeI 363 ACTAGT A^CTAGT
TaqI 170 TCGA T^CGA
TseI 116 GCTGC G^C[AT]GC
Tsp509I 133 AATT ^AATT
TthHB8I 170 TCGA T^CGA
Van91I 432 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 172 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
BstYI 197 AGATCC [GA]^GATC[CT]
319 GGATCT
HapII 299 CCGG C^CGG
305 CCGG
HpaII 299 CCGG C^CGG
305 CCGG
MflI 197 AGATCC [GA]^GATC[CT]
319 GGATCT
MspI 299 CCGG C^CGG
305 CCGG
NlaIII 388 CATG CATG^
448 CATG
SfaNI 3 GATGC GCATC(5/9)
379 GATGC
XhoII 197 AGATCC [GA]^GATC[CT]
319 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AlwI BsiYI BslI BssKI BstNI
DdeI DpnII EcoRII HinfI MboI
MboII MnlI MvaI NdeII NlaIV
Sau3AI ScrFI TfiI