Restriction map for pF1KB6801
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# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstXI       BstZ17I     Cac8I       
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HaeIII      HgaI        Hin1I       
HindIII     HpaI        KasI        KspI        MaeII       
MaeIII      MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tsp45I      TspRI       Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        101  GGTACC               G^GTACC
AccI          367  GTAGAC               GT^[AC][GT]AC
AfaI          102  GTAC                 GT^AC
AluI           11  AGCT                 AG^CT
AlwNI         317  CAGGATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          396  CCTTAGG              CC^T[ACGT]AGG
Asp700I       172  GAACTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       101  GGTACC               G^GTACC
AvaII         160  GGACC                G^G[AT]CC
BanI          101  GGTACC               G^G[CT][GA]CC
BbvI          116  GCTGC                GCAGC(8/12)
BcgI          372  CGAAAGTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          364  CTAG                 C^TAG
BsaBI         314  GATCAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          401  GGTCTC               GGTCTC(1/5)
BsmAI         402  GTCTC                GTCTC(1/5)
BstBI         169  TTCGAA               TT^CGAA
BstF5I         51  CATCC                GGATG(2/0)
Bsu36I        396  CCTTAGG              CC^T[ACGT]AGG
CfoI          114  GCGC                 GCG^C
Cfr13I        160  GGACC                G^G[ACGT]CC
EarI          457  GAAGAG               CTCTTC(1/4)
Eco81I        396  CCTTAGG              CC^T[ACGT]AGG
Fnu4HI        116  GCTGC                GC^[ACGT]GC
FokI           51  CATCC                GGATG(9/13)
HhaI          114  GCGC                 GCG^C
HinP1I        114  GCGC                 G^CGC
HincII        443  GTCAAC               GT[CT]^[GA]AC
HindII        443  GTCAAC               GT[CT]^[GA]AC
HphI          184  GGTGA                GGTGA(8/7)
ItaI          116  GCTGC                GC^[ACGT]GC
KpnI          101  GGTACC               GGTAC^C
Ksp632I       457  GAAGAG               CTCTTC(1/4)
MaeI          364  CTAG                 C^TAG
MamI          314  GATCAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV          169  TTCGAA               TT^CGAA
PflMI         432  CCAGGACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         436  GACTTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          102  GTAC                 GT^AC
Sau96I        160  GGACC                G^G[ACGT]CC
SfuI          169  TTCGAA               TT^CGAA
SpeI          363  ACTAGT               A^CTAGT
TaqI          170  TCGA                 T^CGA
TseI          116  GCTGC                G^C[AT]GC
Tsp509I       133  AATT                 ^AATT
TthHB8I       170  TCGA                 T^CGA
Van91I        432  CCAGGACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          172  GAACTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BstYI         197  AGATCC               [GA]^GATC[CT]
              319  GGATCT              
HapII         299  CCGG                 C^CGG
              305  CCGG                
HpaII         299  CCGG                 C^CGG
              305  CCGG                
MflI          197  AGATCC               [GA]^GATC[CT]
              319  GGATCT              
MspI          299  CCGG                 C^CGG
              305  CCGG                
NlaIII        388  CATG                 CATG^
              448  CATG                
SfaNI           3  GATGC                GCATC(5/9)
              379  GATGC               
XhoII         197  AGATCC               [GA]^GATC[CT]
              319  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AlwI        BsiYI       BslI        BssKI       BstNI       
DdeI        DpnII       EcoRII      HinfI       MboI        
MboII       MnlI        MvaI        NdeII       NlaIV       
Sau3AI      ScrFI       TfiI        
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