Restriction map for
pF1KB0922
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HincII HindII HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 940 GGTACC G^GTACC
AcsI 61 AAATTT [GA]^AATT[CT]
AcyI 67 GACGCC G[GA]^CG[CT]C
Alw44I 145 GTGCAC G^TGCAC
ApaLI 145 GTGCAC G^TGCAC
ApoI 61 AAATTT [GA]^AATT[CT]
Asp718I 940 GGTACC G^GTACC
AspHI 145 GTGCAC G[AT]GC[AT]^C
AspI 847 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 371 TGCGCA TGC^GCA
BalI 242 TGGCCA TGG^CCA
BbsI 672 GAAGAC GAAGAC(2/6)
BglI 497 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 817 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 817 GCTGAGC GC^T[ACGT]AGC
BpuAI 672 GAAGAC GAAGAC(2/6)
BsaAI 590 TACGTG [CT]AC^GT[GA]
BsaHI 67 GACGCC G[GA]^CG[CT]C
BsaI 454 GAGACC GGTCTC(1/5)
BseRI 1010 GAGGAG GAGGAG(10/8)
BsiEI 7 CGGCCG CG[GA][CT]^CG
BsiHKAI 145 GTGCAC G[AT]GC[AT]^C
BsmI 1004 GCATTC GAATGC(1/-1)
BssHII 25 GCGCGC G^CGCGC
BstXI 296 CCAGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 817 GCTGAGC GC^T[ACGT]AGC
EagI 7 CGGCCG C^GGCCG
EarI 54 GAAGAG CTCTTC(1/4)
EclXI 7 CGGCCG C^GGCCG
Eco52I 7 CGGCCG C^GGCCG
EcoT14I 677 CCTTGG C^C[AT][AT]GG
FspI 371 TGCGCA TGC^GCA
Hin1I 67 GACGCC G[GA]^CG[CT]C
KpnI 940 GGTACC GGTAC^C
Ksp632I 54 GAAGAG CTCTTC(1/4)
MluNI 242 TGGCCA TGG^CCA
MscI 242 TGGCCA TGG^CCA
PaeR7I 643 CTCGAG C^TCGAG
PflFI 847 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 546 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 54 GAAGAGC GCTCTTC(1/4)
SexAI 848 ACCTGGT A^CC[AT]GGT
SmaI 156 CCCGGG CCC^GGG
SmlI 643 CTCGAG C^T[CT][GA]AG
StyI 677 CCTTGG C^C[AT][AT]GG
TfiI 569 GAATC G^A[AT]TC
Tsp509I 62 AATT ^AATT
Tth111I 847 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 546 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 643 CTCGAG C^TCGAG
XmaCI 156 CCCGGG C^CCGGG
XmaI 156 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 288 AGCT AG^CT
949 AGCT
AlwI 153 GATCC GGATC(4/5)
639 GATCC
AlwNI 547 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
803 CAGGGCCTG
AvaI 156 CCCGGG C^[CT]CG[GA]G
643 CTCGAG
BanI 363 GGCACC G^G[CT][GA]CC
940 GGTACC
BpmI 294 CTCCAG CTGGAG(16/14)
553 CTGGAG
BsmAI 454 GAGAC GTCTC(1/5)
891 GTCTC
BsmFI 911 GGGAC GGGAC(10/14)
32 GTCCC
BsoBI 156 CCCGGG C^[CT]CG[GA]G
643 CTCGAG
BspMI 200 ACCTGC ACCTGC(4/8)
914 ACCTGC
BsrBI 108 GAGCGG CCGCTC(-3/-3)
556 GAGCGG
BstYI 152 AGATCC [GA]^GATC[CT]
638 AGATCC
DpnII 153 GATC ^GATC
639 GATC
HgaI 67 GACGC GACGC(5/10)
80 GACGC
MboI 153 GATC ^GATC
639 GATC
MflI 152 AGATCC [GA]^GATC[CT]
638 AGATCC
NdeII 153 GATC ^GATC
639 GATC
PpuMI 789 AGGTCCT [GA]G^G[AT]CC[CT]
911 GGGACCT
PstI 325 CTGCAG CTGCA^G
800 CTGCAG
Sau3AI 153 GATC ^GATC
639 GATC
SfcI 325 CTGCAG C^T[GA][CT]AG
800 CTGCAG
XhoII 152 AGATCC [GA]^GATC[CT]
638 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI ApaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TspRI
TthHB8I