################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI BstAPI BstBI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 213 GTATAC GT^[AC][GT]AC AccII 145 CGCG CG^CG AcsI 199 GAATTC [GA]^AATT[CT] AfaI 388 GTAC GT^AC ApoI 199 GAATTC [GA]^AATT[CT] AvaII 29 GGTCC G^G[AT]CC BbsI 283 GAAGAC GAAGAC(2/6) BfaI 463 CTAG C^TAG BglI 406 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 518 AGATCT A^GATCT BpuAI 283 GAAGAC GAAGAC(2/6) BsaAI 389 TACGTG [CT]AC^GT[GA] BsaI 131 GAGACC GGTCTC(1/5) Bst1107I 213 GTATAC GTA^TAC BstEII 120 GGTGACC G^GT[ACGT]ACC BstPI 120 GGTGACC G^GT[ACGT]ACC BstUI 145 CGCG CG^CG BstYI 518 AGATCT [GA]^GATC[CT] BstZ17I 213 GTATAC GTA^TAC Cac8I 294 GCAAGC GC[ACGT]^[ACGT]GC CfoI 146 GCGC GCG^C DraII 28 GGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 142 GACCGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 222 TGGCCG [CT]^GGCC[GA] EarI 565 GAAGAG CTCTTC(1/4) EcoO109I 28 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 120 GGTGACC G^GT[ACGT]ACC EcoRI 199 GAATTC G^AATTC FauI 101 GCGGG CCCGC(4/6) HhaI 146 GCGC GCG^C HinP1I 146 GCGC G^CGC HphI 120 GGTGA GGTGA(8/7) Ksp632I 565 GAAGAG CTCTTC(1/4) MaeI 463 CTAG C^TAG MaeII 390 ACGT A^CGT MflI 518 AGATCT [GA]^GATC[CT] MspA1I 252 CAGCTG C[AC]G^C[GT]G MvnI 145 CGCG CG^CG MwoI 406 GCCCCGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 69 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 568 GAGTC GAGTC(4/5) PpuMI 28 GGGTCCC [GA]G^G[AT]CC[CT] PstI 550 CTGCAG CTGCA^G PvuII 252 CAGCTG CAG^CTG RsaI 388 GTAC GT^AC SfaNI 113 GATGC GCATC(5/9) SmlI 640 CTCAAG C^T[CT][GA]AG TspRI 601 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 69 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 462 TCTAGA T^CTAGA XhoII 518 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 144 CCGC CCGC(-3/-1) 101 GCGG AlwI 350 GATCC GGATC(4/5) 13 GGATC BseRI 180 CTCCTC GAGGAG(10/8) 442 GAGGAG BsmAI 131 GAGAC GTCTC(1/5) 570 GTCTC BspMI 56 ACCTGC ACCTGC(4/8) 593 ACCTGC Cfr13I 29 GGTCC G^G[ACGT]CC 78 GGCCC DdeI 183 CTCAG C^T[ACGT]AG 325 CTGAG DsaI 19 CCATGG C^C[GA][CT]GG 219 CCATGG MaeIII 121 GTGAC ^GT[ACGT]AC 379 GTCAC NcoI 19 CCATGG C^CATGG 219 CCATGG Sau96I 29 GGTCC G^G[ACGT]CC 78 GGCCC SfcI 431 CTGTAG C^T[GA][CT]AG 550 CTGCAG Tsp45I 121 GTGAC ^GT[GC]AC 379 GTCAC Tsp509I 200 AATT ^AATT 634 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AluI BbvI BcnI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspI MvaI NciI NdeII NlaIII NlaIV Sau3AI ScrFI StyI TaqI TfiI TseI TthHB8I