################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HindIII KasI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I TspRI Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 172 CGCG CG^CG AcyI 406 GGCGTC G[GA]^CG[CT]C AhdI 562 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 111 GATCC GGATC(4/5) AlwNI 115 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 562 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 64 GAGCAC G[AT]GC[AT]^C AvaII 264 GGACC G^G[AT]CC BanI 492 GGCACC G^G[CT][GA]CC BlpI 4 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC BsaAI 393 TACGTA [CT]AC^GT[GA] BsaBI 333 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 406 GGCGTC G[GA]^CG[CT]C BsaWI 329 ACCGGA [AT]^CCGG[AT] BsgI 234 GTGCAG GTGCAG(16/14) BsiHKAI 64 GAGCAC G[AT]GC[AT]^C BstUI 172 CGCG CG^CG BstXI 203 CCAACGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 110 AGATCC [GA]^GATC[CT] CelII 4 GCTGAGC GC^T[ACGT]AGC DdeI 5 CTGAG C^T[ACGT]AG DpnII 111 GATC ^GATC DrdI 190 GACCCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 482 CCGTGG C^C[GA][CT]GG EaeI 176 TGGCCG [CT]^GGCC[GA] Eam1105I 562 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 96 GAAGAG CTCTTC(1/4) EcoT14I 497 CCAAGG C^C[AT][AT]GG FauI 536 CCCGC CCCGC(4/6) HgaI 407 GCGTC GACGC(5/10) Hin1I 406 GGCGTC G[GA]^CG[CT]C HinfI 582 GAATC G^A[ACGT]TC HpaI 352 GTTAAC GTT^AAC Ksp632I 96 GAAGAG CTCTTC(1/4) MaeIII 214 GTGAC ^GT[ACGT]AC MamI 333 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 111 GATC ^GATC MboII 96 GAAGA GAAGA(8/7) MflI 110 AGATCC [GA]^GATC[CT] MseI 353 TTAA T^TAA MvnI 172 CGCG CG^CG NdeII 111 GATC ^GATC PflMI 114 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 28 CAGCTG CAG^CTG SapI 96 GAAGAGC GCTCTTC(1/4) Sau3AI 111 GATC ^GATC SfcI 522 CTACAG C^T[GA][CT]AG SnaBI 393 TACGTA TAC^GTA StyI 497 CCAAGG C^C[AT][AT]GG TfiI 582 GAATC G^A[AT]TC Tru9I 353 TTAA T^TAA Tsp45I 214 GTGAC ^GT[GC]AC Van91I 114 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 497 CCAAGGGTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 110 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### ApaI 249 GGGCCC GGGCC^C 448 GGGCCC BpmI 256 CTGGAG CTGGAG(16/14) 280 CTGGAG BsmFI 199 GTCCC GGGAC(10/14) 534 GTCCC BsrI 217 ACTGG ACTGG(1/-1) 602 ACTGG HincII 73 GTCAAC GT[CT]^[GA]AC 352 GTTAAC HindII 73 GTCAAC GT[CT]^[GA]AC 352 GTTAAC HphI 213 GGTGA GGTGA(8/7) 587 TCACC MspA1I 28 CAGCTG C[AC]G^C[GT]G 44 CAGCGG NlaIII 375 CATG CATG^ 432 CATG SfaNI 101 GCATC GCATC(5/9) 527 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI AvaI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MaeII MnlI MspI MvaI MwoI NciI NlaIV RsaI Sau96I ScrFI SmaI TseI XmaCI XmaI