Restriction map for
pF1KB6908
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HaeII HapII HgaI Hin1I HincII
HindII HpaI HpaII HphI KasI
KpnI KspI MaeI MaeII MamI
MboI MfeI MflI MluI MroI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 402 GTATAC GT^[AC][GT]AC
AcsI 620 AAATTC [GA]^AATT[CT]
AfaI 221 GTAC GT^AC
ApoI 620 AAATTC [GA]^AATT[CT]
AviII 44 TGCGCA TGC^GCA
BalI 149 TGGCCA TGG^CCA
BpmI 568 CTGGAG CTGGAG(16/14)
BsaI 304 GAGACC GGTCTC(1/5)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsmAI 304 GAGAC GTCTC(1/5)
BsmFI 424 GGGAC GGGAC(10/14)
BsrI 567 ACTGG ACTGG(1/-1)
BssKI 309 CCTGG ^CC[ACGT]GG
Bst1107I 402 GTATAC GTA^TAC
BstNI 309 CCTGG CC^[AT]GG
BstZ17I 402 GTATAC GTA^TAC
Cac8I 21 GCGGGC GC[ACGT]^[ACGT]GC
CfoI 45 GCGC GCG^C
DsaI 32 CCGTGG C^C[GA][CT]GG
EagI 8 CGGCCG C^GGCCG
EarI 301 GAAGAG CTCTTC(1/4)
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoNI 309 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 309 CCTGG ^CC[AT]GG
FauI 21 GCGGG CCCGC(4/6)
FspI 44 TGCGCA TGC^GCA
HhaI 45 GCGC GCG^C
HinP1I 45 GCGC G^CGC
HindIII 265 AAGCTT A^AGCTT
HinfI 103 GATTC G^A[ACGT]TC
Ksp632I 301 GAAGAG CTCTTC(1/4)
MaeIII 178 GTTAC ^GT[ACGT]AC
MluNI 149 TGGCCA TGG^CCA
MscI 149 TGGCCA TGG^CCA
MvaI 309 CCTGG CC^[AT]GG
NlaIV 318 GGGTCC GG[ACGT]^[ACGT]CC
PstI 85 CTGCAG CTGCA^G
RsaI 221 GTAC GT^AC
ScrFI 309 CCTGG CC^[ACGT]GG
SfaNI 613 GATGC GCATC(5/9)
SfcI 85 CTGCAG C^T[GA][CT]AG
SspI 442 AATATT AAT^ATT
TaqI 564 TCGA T^CGA
TfiI 103 GATTC G^A[AT]TC
TthHB8I 564 TCGA T^CGA
XcmI 16 CCACAGCGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BsaJI 32 CCGTGG C^C[ACGT][ACGT]GG
308 CCCTGG
BsiYI 309 CCTGGATGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
373 CCCAAAAAGGG
BslI 309 CCTGGATGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
373 CCCAAAAAGGG
DdeI 206 CTAAG C^T[ACGT]AG
245 CTGAG
EaeI 8 CGGCCG [CT]^GGCC[GA]
149 TGGCCA
HaeIII 9 GGCC GG^CC
150 GGCC
MnlI 316 GAGG CCTC(7/6)
518 GAGG
MwoI 45 GCGCAGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
151 GCCAAGACAGC
PvuII 40 CAGCTG CAG^CTG
55 CAGCTG
TspRI 48 CAGTG CA[GC]TG[ACGT][ACGT]^
394 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AluI AvaII BbvI BstF5I
Cfr13I Fnu4HI FokI ItaI MboII
MseI MspA1I Sau96I Tru9I TseI
Tsp509I