Restriction map for
pF1KB9950
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AviII
AvrII BanI BbeI BbvCI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraI DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoRV FauI
FseI FspI HaeII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AciI 1164 CCGC CCGC(-3/-1)
AcyI 213 GACGCC G[GA]^CG[CT]C
AhdI 1201 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1500 CCTGAGG CC^T[ACGT]AGG
AspEI 1201 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1275 TGGCCA TGG^CCA
BanII 1347 GGGCTC G[GA]GC[CT]^C
BbrPI 1048 CACGTG CAC^GTG
BciVI 3 GGATAC GTATCC(6/5)
BcnI 1602 CCGGG CC^[GC]GG
BglII 1638 AGATCT A^GATCT
BmyI 1347 GGGCTC G[AGT]GC[ACT]^C
BsaAI 1048 CACGTG [CT]AC^GT[GA]
BsaBI 908 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 213 GACGCC G[GA]^CG[CT]C
BsaI 1872 GGTCTC GGTCTC(1/5)
Bsp1286I 1347 GGGCTC G[AGT]GC[ACT]^C
BspMI 344 ACCTGC ACCTGC(4/8)
BsrDI 1478 GCAATG GCAATG(2/0)
BsrFI 1895 ACCGGC [GA]^CCGG[CT]
BssSI 1948 CACGAG CACGAG(-5/-1)
BstAPI 1478 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 334 GGTAACC G^GT[ACGT]ACC
BstPI 334 GGTAACC G^GT[ACGT]ACC
BstXI 1723 CCAGTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1500 CCTGAGG CC^T[ACGT]AGG
CfoI 640 GCGC GCG^C
Cfr10I 1895 ACCGGC [GA]^CCGG[CT]
DraII 1193 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1168 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1690 GACCAGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1275 TGGCCA [CT]^GGCC[GA]
Eam1105I 1201 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1500 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1193 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 334 GGTAACC G^GT[ACGT]ACC
EcoT14I 706 CCAAGG C^C[AT][AT]GG
EcoT22I 275 ATGCAT ATGCA^T
HhaI 640 GCGC GCG^C
Hin1I 213 GACGCC G[GA]^CG[CT]C
HinP1I 640 GCGC G^CGC
HincII 1176 GTCAAC GT[CT]^[GA]AC
HindII 1176 GTCAAC GT[CT]^[GA]AC
HindIII 1114 AAGCTT A^AGCTT
MamI 908 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1275 TGGCCA TGG^CCA
MscI 1275 TGGCCA TGG^CCA
MslI 1054 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1602 CCGGG CC^[GC]GG
NdeI 443 CATATG CA^TATG
NsiI 275 ATGCAT ATGCA^T
NspI 692 ACATGC [GA]CATG^[CT]
PaeR7I 202 CTCGAG C^TCGAG
PflMI 342 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1048 CACGTG CAC^GTG
PmlI 1048 CACGTG CAC^GTG
PpuMI 1193 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 461 CTGCAG CTGCA^G
SmlI 202 CTCGAG C^T[CT][GA]AG
StyI 706 CCAAGG C^C[AT][AT]GG
Van91I 342 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 18 TCTAGA T^CTAGA
XhoI 202 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 527 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1938 CAGCTGCTG
AvaI 202 CTCGAG C^[CT]CG[GA]G
521 CTCGGG
AvaII 1194 GGTCC G^G[AT]CC
1290 GGTCC
BamHI 157 GGATCC G^GATCC
1796 GGATCC
BbsI 712 GAAGAC GAAGAC(2/6)
1989 GTCTTC
BclI 1151 TGATCA T^GATCA
1337 TGATCA
BpmI 903 CTCCAG CTGGAG(16/14)
1739 CTCCAG
BpuAI 712 GAAGAC GAAGAC(2/6)
1989 GTCTTC
BseRI 205 GAGGAG GAGGAG(10/8)
1503 GAGGAG
BsmAI 225 GAGAC GTCTC(1/5)
1873 GTCTC
BsoBI 202 CTCGAG C^[CT]CG[GA]G
521 CTCGGG
Bsp1407I 986 TGTACA T^GTACA
1934 TGTACA
BsrGI 986 TGTACA T^GTACA
1934 TGTACA
EcoRI 832 GAATTC G^AATTC
1366 GAATTC
FbaI 1151 TGATCA T^GATCA
1337 TGATCA
HapII 1602 CCGG C^CGG
1896 CCGG
HgaI 213 GACGC GACGC(5/10)
1965 GCGTC
HpaII 1602 CCGG C^CGG
1896 CCGG
HphI 64 TCACC GGTGA(8/7)
1587 TCACC
MaeII 1049 ACGT A^CGT
1781 ACGT
MspA1I 1294 CAGCTG C[AC]G^C[GT]G
1938 CAGCTG
MspI 1602 CCGG C^CGG
1896 CCGG
PvuII 1294 CAGCTG CAG^CTG
1938 CAGCTG
SfcI 218 CTGTAG C^T[GA][CT]AG
461 CTGCAG
SspBI 986 TGTACA T^GTACA
1934 TGTACA
TaqI 203 TCGA T^CGA
970 TCGA
TthHB8I 203 TCGA T^CGA
970 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BfaI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI