Restriction map for
pF1KB3940
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstNI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoRV FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MluI MluNI MroI MscI
MseI MunI MvaI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI Tru9I
Tsp45I Tth111I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 427 GTAGAC GT^[AC][GT]AC
AvaI 17 CCCGGG C^[CT]CG[GA]G
BamHI 228 GGATCC G^GATCC
BsoBI 17 CCCGGG C^[CT]CG[GA]G
BsrI 164 ACTGG ACTGG(1/-1)
BssSI 93 CACGAG CACGAG(-5/-1)
BstAPI 132 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 196 GGTTACC G^GT[ACGT]ACC
BstPI 196 GGTTACC G^GT[ACGT]ACC
BstYI 228 GGATCC [GA]^GATC[CT]
Cfr13I 20 GGGCC G^G[ACGT]CC
DdeI 265 CTGAG C^T[ACGT]AG
DrdI 263 GACTGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 196 GGTTACC G^GT[ACGT]ACC
EcoT14I 83 CCAAGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
HaeIII 21 GGCC GG^CC
HapII 18 CCGG C^CGG
HincII 316 GTTGAC GT[CT]^[GA]AC
HindII 316 GTTGAC GT[CT]^[GA]AC
HinfI 437 GAATC G^A[ACGT]TC
HpaII 18 CCGG C^CGG
HphI 382 TCACC GGTGA(8/7)
MaeII 416 ACGT A^CGT
MaeIII 197 GTTAC ^GT[ACGT]AC
MflI 228 GGATCC [GA]^GATC[CT]
MspI 18 CCGG C^CGG
NsiI 1 ATGCAT ATGCA^T
PflMI 219 CCATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 20 GGGCC G^G[ACGT]CC
SbfI 129 CCTGCAGG CCTGCA^GG
SmaI 17 CCCGGG CCC^GGG
Sse8387I 129 CCTGCAGG CCTGCA^GG
StyI 83 CCAAGG C^C[AT][AT]GG
TfiI 437 GAATC G^A[AT]TC
Tsp509I 342 AATT ^AATT
TspRI 163 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 219 CCATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 413 CCAACGTGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 228 GGATCC [GA]^GATC[CT]
XmaCI 17 CCCGGG C^CCGGG
XmaI 17 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanII 88 GGGCTC G[GA]GC[CT]^C
474 GAGCCC
BcnI 17 CCCGG CC^[GC]GG
18 CCGGG
BmyI 88 GGGCTC G[AGT]GC[ACT]^C
474 GAGCCC
BsaJI 17 CCCGGG C^C[ACGT][ACGT]GG
83 CCAAGG
Bsp1286I 88 GGGCTC G[AGT]GC[ACT]^C
474 GAGCCC
BssKI 17 CCCGG ^CC[ACGT]GG
18 CCGGG
BstF5I 4 CATCC GGATG(2/0)
351 GGATG
FokI 4 CATCC GGATG(9/13)
351 GGATG
MslI 360 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
411 CACCAACGTG
NciI 17 CCCGG CC^[GC]GG
18 CCGGG
NlaIII 30 CATG CATG^
182 CATG
PstI 109 CTGCAG CTGCA^G
130 CTGCAG
PvuII 106 CAGCTG CAG^CTG
441 CAGCTG
ScrFI 17 CCCGG CC^[ACGT]GG
18 CCGGG
SfaNI 3 GCATC GCATC(5/9)
212 GCATC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI AlwI AlwNI BbvI
BsiYI BslI Cac8I DpnII Fnu4HI
ItaI MboI MnlI MspA1I MwoI
NdeII NlaIV Sau3AI SfcI TseI