Restriction map for
pF1KB3491
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BbeI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoRV FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 1651 CGCG CG^CG
AflIII 1542 ACACGT A^C[GA][CT]GT
AhdI 1388 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 122 AGCGCT AGC^GCT
ApaI 1714 GGGCCC GGGCC^C
AspEI 1388 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1745 CTCGAG C^[CT]CG[GA]G
AvaII 1387 GGACC G^G[AT]CC
BalI 1656 TGGCCA TGG^CCA
BamHI 33 GGATCC G^GATCC
BanI 1447 GGTGCC G^G[CT][GA]CC
BbrPI 1543 CACGTG CAC^GTG
BcgI 661 GCATTTAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1543 CACGTG [CT]AC^GT[GA]
BsaWI 419 ACCGGA [AT]^CCGG[AT]
BseRI 595 GAGGAG GAGGAG(10/8)
BsmI 1058 GAATGC GAATGC(1/-1)
BsoBI 1745 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1103 TGTACA T^GTACA
BspHI 39 TCATGA T^CATGA
BsrGI 1103 TGTACA T^GTACA
BstF5I 204 GGATG GGATG(2/0)
BstUI 1651 CGCG CG^CG
BstXI 1331 CCAACCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1554 TTTAAA TTT^AAA
EaeI 1656 TGGCCA [CT]^GGCC[GA]
Eam1105I 1388 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1256 GAAGAG CTCTTC(1/4)
Eco47III 122 AGCGCT AGC^GCT
EcoNI 937 CCTGGGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 445 GAATTC G^AATTC
EcoT22I 659 ATGCAT ATGCA^T
FokI 204 GGATG GGATG(9/13)
Ksp632I 1256 GAAGAG CTCTTC(1/4)
MfeI 472 CAATTG C^AATTG
MluNI 1656 TGGCCA TGG^CCA
MscI 1656 TGGCCA TGG^CCA
MunI 472 CAATTG C^AATTG
MvnI 1651 CGCG CG^CG
NcoI 1577 CCATGG C^CATGG
NsiI 659 ATGCAT ATGCA^T
PaeR7I 1745 CTCGAG C^TCGAG
PmaCI 1543 CACGTG CAC^GTG
PmlI 1543 CACGTG CAC^GTG
PpuMI 1386 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 926 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 39 TCATGA T^CATGA
ScaI 73 AGTACT AGT^ACT
SexAI 635 ACCAGGT A^CC[AT]GGT
SmlI 1745 CTCGAG C^T[CT][GA]AG
SspBI 1103 TGTACA T^GTACA
XhoI 1745 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 74 GTAC GT^AC
1104 GTAC
AspHI 254 GTGCTC G[AT]GC[AT]^C
1411 GTGCTC
BanII 996 GGGCTC G[GA]GC[CT]^C
1714 GGGCCC
BbsI 109 GAAGAC GAAGAC(2/6)
1050 GAAGAC
BbvCI 1718 CCTCAGC CCTCAGC(-5/-2)
886 GCTGAGG
BciVI 1912 GGATAC GTATCC(6/5)
1032 GTATCC
BclI 192 TGATCA T^GATCA
1899 TGATCA
BcnI 1416 CCGGG CC^[GC]GG
1704 CCGGG
BglI 877 GCCCCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
951 GCCTATCTGGC
BglII 568 AGATCT A^GATCT
1041 AGATCT
BpuAI 109 GAAGAC GAAGAC(2/6)
1050 GAAGAC
BsgI 13 GTGCAG GTGCAG(16/14)
169 GTGCAG
BsiHKAI 254 GTGCTC G[AT]GC[AT]^C
1411 GTGCTC
BspMI 1582 GCAGGT ACCTGC(4/8)
1638 GCAGGT
BsrDI 1510 CATTGC GCAATG(2/0)
1872 CATTGC
BstEII 558 GGTTACC G^GT[ACGT]ACC
1605 GGTGACC
BstPI 558 GGTTACC G^GT[ACGT]ACC
1605 GGTGACC
DsaI 1382 CCACGG C^C[GA][CT]GG
1577 CCATGG
EcoO65I 558 GGTTACC G^GT[ACGT]ACC
1605 GGTGACC
FauI 1649 CCCGC CCCGC(4/6)
1840 CCCGC
FbaI 192 TGATCA T^GATCA
1899 TGATCA
MaeII 1544 ACGT A^CGT
1933 ACGT
MslI 1023 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1903 CACATCGGTG
NciI 1416 CCGGG CC^[GC]GG
1704 CCGGG
NspI 480 ACATGC [GA]CATG^[CT]
575 ACATGC
RsaI 74 GTAC GT^AC
1104 GTAC
StuI 949 AGGCCT AGG^CCT
1830 AGGCCT
XcmI 266 CCATGTTCATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1068 CCAGCGTGGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII