Restriction map for
pF1KE0922
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BbvCI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BsmBI BsmI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CpoI EagI EclXI Eco52I Eco81I
EcoO65I FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI MvnI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 1918 GAATTC [GA]^AATT[CT]
AflIII 1633 ACACGT A^C[GA][CT]GT
AhdI 1246 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1110 AGCGCT AGC^GCT
ApoI 1918 GAATTC [GA]^AATT[CT]
AspEI 1246 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1496 GGATCC G^GATCC
BanI 19 GGTGCC G^G[CT][GA]CC
BcgI 624 GCAGCATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 911 TGATCA T^GATCA
BfaI 1607 CTAG C^TAG
BsgI 1037 GTGCAG GTGCAG(16/14)
Bsp1407I 86 TGTACA T^GTACA
BspDI 1159 ATCGAT AT^CGAT
BsrBI 415 GAGCGG CCGCTC(-3/-3)
BsrGI 86 TGTACA T^GTACA
BstAPI 13 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1868 CCAGGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1159 ATCGAT AT^CGAT
DraI 219 TTTAAA TTT^AAA
DrdI 1209 GACCTTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1246 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1110 AGCGCT AGC^GCT
EcoRI 1918 GAATTC G^AATTC
EcoRV 1162 GATATC GAT^ATC
EcoT22I 1763 ATGCAT ATGCA^T
FbaI 911 TGATCA T^GATCA
HaeII 1110 AGCGCT [GA]GCGC^[CT]
HgaI 1096 GACGC GACGC(5/10)
MaeI 1607 CTAG C^TAG
NaeI 1351 GCCGGC GCC^GGC
NcoI 1340 CCATGG C^CATGG
NgoMIV 1351 GCCGGC G^CCGGC
NsiI 1763 ATGCAT ATGCA^T
NspI 854 ACATGC [GA]CATG^[CT]
PflMI 384 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 385 CAGCTG CAG^CTG
SacI 1467 GAGCTC GAGCT^C
SapI 777 GCTCTTC GCTCTTC(1/4)
SexAI 524 ACCTGGT A^CC[AT]GGT
SfcI 903 CTACAG C^T[GA][CT]AG
SspBI 86 TGTACA T^GTACA
SspI 761 AATATT AAT^ATT
TfiI 1284 GATTC G^A[AT]TC
Van91I 384 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1709 CCAACTATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BciVI 743 GTATCC GTATCC(6/5)
1879 GTATCC
BglI 1567 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1990 GCCTTGCTGGC
BsaBI 646 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
822 GATCCGCATC
BsaI 1268 GAGACC GGTCTC(1/5)
834 GGTCTC
BspHI 1295 TCATGA T^CATGA
1640 TCATGA
BsrDI 210 CATTGC GCAATG(2/0)
1181 GCAATG
BsrFI 1305 ACCGGC [GA]^CCGG[CT]
1351 GCCGGC
Cfr10I 1305 ACCGGC [GA]^CCGG[CT]
1351 GCCGGC
DraIII 14 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1604 CACCTAGTG
DsaI 1340 CCATGG C^C[GA][CT]GG
1514 CCACGG
EarI 778 CTCTTC CTCTTC(1/4)
1804 CTCTTC
EcoT14I 1340 CCATGG C^C[AT][AT]GG
1625 CCTTGG
FauI 1883 CCCGC CCCGC(4/6)
417 GCGGG
Ksp632I 778 CTCTTC CTCTTC(1/4)
1804 CTCTTC
MaeIII 113 GTGAC ^GT[ACGT]AC
1964 GTGAC
MamI 646 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
822 GATCCGCATC
MspA1I 385 CAGCTG C[AC]G^C[GT]G
1189 CAGCGG
RcaI 1295 TCATGA T^CATGA
1640 TCATGA
StuI 1424 AGGCCT AGG^CCT
1988 AGGCCT
StyI 1340 CCATGG C^C[AT][AT]GG
1625 CCTTGG
Tsp45I 113 GTGAC ^GT[GC]AC
1964 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BglII BmyI
BpmI BpuAI BsaJI BsaWI BseAI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII