################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AgeI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbeI BbrPI BbvCI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BsiEI BsiWI BsmBI BsmI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CpoI EagI EclXI Eco52I Eco81I EcoO65I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 1918 GAATTC [GA]^AATT[CT] AflIII 1633 ACACGT A^C[GA][CT]GT AhdI 1246 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1110 AGCGCT AGC^GCT ApoI 1918 GAATTC [GA]^AATT[CT] AspEI 1246 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1496 GGATCC G^GATCC BanI 19 GGTGCC G^G[CT][GA]CC BcgI 624 GCAGCATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 911 TGATCA T^GATCA BfaI 1607 CTAG C^TAG BsgI 1037 GTGCAG GTGCAG(16/14) Bsp1407I 86 TGTACA T^GTACA BspDI 1159 ATCGAT AT^CGAT BsrBI 415 GAGCGG CCGCTC(-3/-3) BsrGI 86 TGTACA T^GTACA BstAPI 13 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1868 CCAGGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1159 ATCGAT AT^CGAT DraI 219 TTTAAA TTT^AAA DrdI 1209 GACCTTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1246 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1110 AGCGCT AGC^GCT EcoRI 1918 GAATTC G^AATTC EcoRV 1162 GATATC GAT^ATC EcoT22I 1763 ATGCAT ATGCA^T FbaI 911 TGATCA T^GATCA HaeII 1110 AGCGCT [GA]GCGC^[CT] HgaI 1096 GACGC GACGC(5/10) MaeI 1607 CTAG C^TAG NaeI 1351 GCCGGC GCC^GGC NcoI 1340 CCATGG C^CATGG NgoMIV 1351 GCCGGC G^CCGGC NsiI 1763 ATGCAT ATGCA^T NspI 854 ACATGC [GA]CATG^[CT] PflMI 384 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 385 CAGCTG CAG^CTG SacI 1467 GAGCTC GAGCT^C SapI 777 GCTCTTC GCTCTTC(1/4) SexAI 524 ACCTGGT A^CC[AT]GGT SfcI 903 CTACAG C^T[GA][CT]AG SspBI 86 TGTACA T^GTACA SspI 761 AATATT AAT^ATT TfiI 1284 GATTC G^A[AT]TC Van91I 384 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1709 CCAACTATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BciVI 743 GTATCC GTATCC(6/5) 1879 GTATCC BglI 1567 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1990 GCCTTGCTGGC BsaBI 646 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 822 GATCCGCATC BsaI 1268 GAGACC GGTCTC(1/5) 834 GGTCTC BspHI 1295 TCATGA T^CATGA 1640 TCATGA BsrDI 210 CATTGC GCAATG(2/0) 1181 GCAATG BsrFI 1305 ACCGGC [GA]^CCGG[CT] 1351 GCCGGC Cfr10I 1305 ACCGGC [GA]^CCGG[CT] 1351 GCCGGC DraIII 14 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1604 CACCTAGTG DsaI 1340 CCATGG C^C[GA][CT]GG 1514 CCACGG EarI 778 CTCTTC CTCTTC(1/4) 1804 CTCTTC EcoT14I 1340 CCATGG C^C[AT][AT]GG 1625 CCTTGG FauI 1883 CCCGC CCCGC(4/6) 417 GCGGG Ksp632I 778 CTCTTC CTCTTC(1/4) 1804 CTCTTC MaeIII 113 GTGAC ^GT[ACGT]AC 1964 GTGAC MamI 646 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 822 GATCCGCATC MspA1I 385 CAGCTG C[AC]G^C[GT]G 1189 CAGCGG RcaI 1295 TCATGA T^CATGA 1640 TCATGA StuI 1424 AGGCCT AGG^CCT 1988 AGGCCT StyI 1340 CCATGG C^C[AT][AT]GG 1625 CCTTGG Tsp45I 113 GTGAC ^GT[GC]AC 1964 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccIII AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BglII BmyI BpmI BpuAI BsaJI BsaWI BseAI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII