################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 564 GTAC GT^AC AlwI 660 GGATC GGATC(4/5) AlwNI 658 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 787 GAGCTC G[AT]GC[AT]^C AvaII 467 GGTCC G^G[AT]CC BbsI 487 GAAGAC GAAGAC(2/6) BbvCI 426 GCTGAGG CCTCAGC(-5/-2) BciVI 651 GTATCC GTATCC(6/5) BcnI 591 CCGGG CC^[GC]GG BglI 108 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 32 AGATCT A^GATCT BpuAI 487 GAAGAC GAAGAC(2/6) BsgI 696 CTGCAC GTGCAG(16/14) BsiHKAI 787 GAGCTC G[AT]GC[AT]^C BsmAI 245 GTCTC GTCTC(1/5) BspMI 611 ACCTGC ACCTGC(4/8) Bst1107I 583 GTATAC GTA^TAC BstF5I 299 GGATG GGATG(2/0) BstXI 470 CCATGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 583 GTATAC GTA^TAC DraII 68 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoNI 318 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 68 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 278 CCTTGG C^C[AT][AT]GG FauI 719 CCCGC CCCGC(4/6) FokI 299 GGATG GGATG(9/13) HapII 591 CCGG C^CGG HincII 775 GTCAAC GT[CT]^[GA]AC HindII 775 GTCAAC GT[CT]^[GA]AC HpaII 591 CCGG C^CGG HphI 666 GGTGA GGTGA(8/7) MseI 445 TTAA T^TAA MslI 766 CATTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 199 CCGCTG C[AC]G^C[GT]G MspI 591 CCGG C^CGG NciI 591 CCGGG CC^[GC]GG PflMI 657 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 346 GAGTC GAGTC(4/5) RsaI 564 GTAC GT^AC SacI 787 GAGCTC GAGCT^C SapI 309 GCTCTTC GCTCTTC(1/4) SfcI 319 CTACAG C^T[GA][CT]AG SmlI 462 CTCAAG C^T[CT][GA]AG StyI 278 CCTTGG C^C[AT][AT]GG TfiI 811 GAATC G^A[AT]TC Tru9I 445 TTAA T^TAA Van91I 657 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### ApaI 68 GGGCCC GGGCC^C 716 GGGCCC BclI 545 TGATCA T^GATCA 762 TGATCA BpmI 74 CTCCAG CTGGAG(16/14) 515 CTGGAG BstYI 32 AGATCT [GA]^GATC[CT] 660 GGATCT EarI 310 CTCTTC CTCTTC(1/4) 216 GAAGAG FbaI 545 TGATCA T^GATCA 762 TGATCA HinfI 346 GAGTC G^A[ACGT]TC 811 GAATC Ksp632I 310 CTCTTC CTCTTC(1/4) 216 GAAGAG MaeII 779 ACGT A^CGT 820 ACGT MflI 32 AGATCT [GA]^GATC[CT] 660 GGATCT SfaNI 360 GATGC GCATC(5/9) 370 GCATC StuI 115 AGGCCT AGG^CCT 237 AGGCCT XhoII 32 AGATCT [GA]^GATC[CT] 660 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AccI AciI AluI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp509I TspRI