################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BbrPI BciVI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 262 GACGTC GACGT^C AccI 156 GTATAC GT^[AC][GT]AC AcyI 262 GACGTC G[GA]^CG[CT]C AhdI 1114 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1114 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 435 CCCGGG C^[CT]CG[GA]G BalI 417 TGGCCA TGG^CCA BbsI 105 GAAGAC GAAGAC(2/6) BbvCI 942 CCTCAGC CCTCAGC(-5/-2) BcgI 231 CGAACAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 754 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 105 GAAGAC GAAGAC(2/6) BsaHI 262 GACGTC G[GA]^CG[CT]C BsaI 985 GAGACC GGTCTC(1/5) BsmBI 934 GAGACG CGTCTC(1/5) BsmI 358 GAATGC GAATGC(1/-1) BsoBI 435 CCCGGG C^[CT]CG[GA]G BspMI 988 ACCTGC ACCTGC(4/8) BsrBI 634 GAGCGG CCGCTC(-3/-3) BsrFI 459 ACCGGC [GA]^CCGG[CT] Bst1107I 156 GTATAC GTA^TAC BstF5I 793 CATCC GGATG(2/0) BstZ17I 156 GTATAC GTA^TAC Cfr10I 459 ACCGGC [GA]^CCGG[CT] DsaI 49 CCGTGG C^C[GA][CT]GG EaeI 417 TGGCCA [CT]^GGCC[GA] Eam1105I 1114 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 645 GCGGG CCCGC(4/6) FokI 793 CATCC GGATG(9/13) Hin1I 262 GACGTC G[GA]^CG[CT]C HincII 1222 GTCAAC GT[CT]^[GA]AC HindII 1222 GTCAAC GT[CT]^[GA]AC MaeII 263 ACGT A^CGT MluNI 417 TGGCCA TGG^CCA MscI 417 TGGCCA TGG^CCA PflMI 1199 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1151 CTGCAG CTGCA^G SexAI 14 ACCTGGT A^CC[AT]GGT SfcI 1151 CTGCAG C^T[GA][CT]AG SmaI 435 CCCGGG CCC^GGG SmlI 916 CTCAAG C^T[CT][GA]AG StuI 317 AGGCCT AGG^CCT Van91I 1199 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 435 CCCGGG C^CCGGG XmaI 435 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AfaI 54 GTAC GT^AC 1056 GTAC AlwI 939 GATCC GGATC(4/5) 117 GGATC AspHI 586 GAGCAC G[AT]GC[AT]^C 731 GAGCAC AvaII 66 GGACC G^G[AT]CC 1161 GGACC BclI 378 TGATCA T^GATCA 519 TGATCA BcnI 435 CCCGG CC^[GC]GG 436 CCGGG BfaI 301 CTAG C^TAG 748 CTAG BglII 78 AGATCT A^GATCT 1283 AGATCT BlpI 94 GCTGAGC GC^T[ACGT]AGC 624 GCTGAGC Bpu1102I 94 GCTGAGC GC^T[ACGT]AGC 624 GCTGAGC BsgI 1036 CTGCAC GTGCAG(16/14) 1135 GTGCAG BsiHKAI 586 GAGCAC G[AT]GC[AT]^C 731 GAGCAC BsmAI 934 GAGAC GTCTC(1/5) 985 GAGAC BssSI 979 CACGAG CACGAG(-5/-1) 673 CTCGTG BstYI 78 AGATCT [GA]^GATC[CT] 1283 AGATCT CelII 94 GCTGAGC GC^T[ACGT]AGC 624 GCTGAGC DraII 65 AGGACCT [GA]G^G[ACGT]CC[CT] 1160 AGGACCT DraIII 508 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG 1039 CACGAAGTG EcoNI 15 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 989 CCTGCATCAGG EcoO109I 65 AGGACCT [GA]G^G[ACGT]CC[CT] 1160 AGGACCT FbaI 378 TGATCA T^GATCA 519 TGATCA HaeII 738 GGCGCT [GA]GCGC^[CT] 1060 AGCGCC MaeI 301 CTAG C^TAG 748 CTAG MflI 78 AGATCT [GA]^GATC[CT] 1283 AGATCT MseI 679 TTAA T^TAA 1277 TTAA NciI 435 CCCGG CC^[GC]GG 436 CCGGG NspI 237 ACATGC [GA]CATG^[CT] 475 GCATGT PpuMI 65 AGGACCT [GA]G^G[AT]CC[CT] 1160 AGGACCT PvuII 622 CAGCTG CAG^CTG 706 CAGCTG RsaI 54 GTAC GT^AC 1056 GTAC TfiI 333 GATTC G^A[AT]TC 898 GAATC Tru9I 679 TTAA T^TAA 1277 TTAA Tsp509I 186 AATT ^AATT 257 AATT XhoII 78 AGATCT [GA]^GATC[CT] 1283 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwNI BanI BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI