Restriction map for
pF1KB5288
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccI AclI AcsI AcyI
AflII AflIII AgeI Alw44I ApaLI
ApoI AscI AseI AsnI AspI
AviII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BsaAI BsaBI BsaHI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
MaeII MamI MfeI MluI MluNI
MscI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tru9I Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 901 GGTACC G^GTACC
AccII 313 CGCG CG^CG
AhdI 1152 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 299 AGCGCT AGC^GCT
ApaI 572 GGGCCC GGGCC^C
Asp700I 1511 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 901 GGTACC G^GTACC
AspEI 1152 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 499 GTGCTC G[AT]GC[AT]^C
AvaI 1052 CTCGAG C^[CT]CG[GA]G
AvrII 1549 CCTAGG C^CTAGG
BanI 901 GGTACC G^G[CT][GA]CC
BbsI 258 GAAGAC GAAGAC(2/6)
BglI 1456 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1549 CCTAGG C^CTAGG
BpuAI 258 GAAGAC GAAGAC(2/6)
BsaI 1503 GAGACC GGTCTC(1/5)
BsgI 913 GTGCAG GTGCAG(16/14)
BsiEI 315 CGGCCG CG[GA][CT]^CG
BsiHKAI 499 GTGCTC G[AT]GC[AT]^C
BsoBI 1052 CTCGAG C^[CT]CG[GA]G
BspMI 246 GCAGGT ACCTGC(4/8)
BsrBI 627 CCGCTC CCGCTC(-3/-3)
BstUI 313 CGCG CG^CG
BstXI 346 CCACTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 104 AGATCC [GA]^GATC[CT]
EaeI 315 CGGCCG [CT]^GGCC[GA]
EagI 315 CGGCCG C^GGCCG
Eam1105I 1152 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 315 CGGCCG C^GGCCG
Eco47III 299 AGCGCT AGC^GCT
Eco52I 315 CGGCCG C^GGCCG
EcoNI 632 CCTCAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1549 CCTAGG C^C[AT][AT]GG
HaeII 299 AGCGCT [GA]GCGC^[CT]
HincII 1039 GTCAAC GT[CT]^[GA]AC
HindII 1039 GTCAAC GT[CT]^[GA]AC
KpnI 901 GGTACC GGTAC^C
KspI 312 CCGCGG CCGC^GG
MflI 104 AGATCC [GA]^GATC[CT]
MvnI 313 CGCG CG^CG
NaeI 266 GCCGGC GCC^GGC
NgoMIV 266 GCCGGC G^CCGGC
PaeR7I 1052 CTCGAG C^TCGAG
PshAI 1050 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 421 CAGCTG CAG^CTG
SacII 312 CCGCGG CCGC^GG
SmlI 1052 CTCGAG C^T[CT][GA]AG
SspI 368 AATATT AAT^ATT
StuI 1602 AGGCCT AGG^CCT
StyI 1549 CCTAGG C^C[AT][AT]GG
TaqI 1053 TCGA T^CGA
Tsp509I 1251 AATT ^AATT
TthHB8I 1053 TCGA T^CGA
XhoI 1052 CTCGAG C^TCGAG
XhoII 104 AGATCC [GA]^GATC[CT]
XmnI 1511 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 107 TCCGGA T^CCGGA
338 TCCGGA
AfaI 902 GTAC GT^AC
1308 GTAC
BanII 572 GGGCCC G[GA]GC[CT]^C
1553 GGGCTC
BlpI 295 GCTGAGC GC^T[ACGT]AGC
865 GCTCAGC
Bpu1102I 295 GCTGAGC GC^T[ACGT]AGC
865 GCTCAGC
BsaWI 107 TCCGGA [AT]^CCGG[AT]
338 TCCGGA
BseAI 107 TCCGGA T^CCGGA
338 TCCGGA
BseRI 630 CTCCTC GAGGAG(10/8)
811 GAGGAG
BsmFI 686 GTCCC GGGAC(10/14)
1531 GTCCC
BspEI 107 TCCGGA T^CCGGA
338 TCCGGA
BspHI 807 TCATGA T^CATGA
1244 TCATGA
BsrDI 494 GCAATG GCAATG(2/0)
1411 GCAATG
BsrFI 266 GCCGGC [GA]^CCGG[CT]
986 ACCGGC
CelII 295 GCTGAGC GC^T[ACGT]AGC
865 GCTCAGC
Cfr10I 266 GCCGGC [GA]^CCGG[CT]
986 ACCGGC
DsaI 312 CCGCGG C^C[GA][CT]GG
1591 CCACGG
MroI 107 TCCGGA T^CCGGA
338 TCCGGA
MslI 347 CACTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
444 CACAGAGATG
RcaI 807 TCATGA T^CATGA
1244 TCATGA
RsaI 902 GTAC GT^AC
1308 GTAC
SapI 1015 GAAGAGC GCTCTTC(1/4)
501 GCTCTTC
TfiI 342 GATTC G^A[AT]TC
776 GATTC
XcmI 746 CCACACTCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1468 CCAGCCCCAGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
AvaII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I TspRI Van91I