################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BciVI BclI BfrI BlnI BsaBI BsaHI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacI SalI SapI ScaI SexAI SfoI SfuI SgrAI SnaBI SphI SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 740 TCCGGA T^CCGGA AclI 835 AACGTT AA^CGTT AlwNI 929 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 736 GTGCTC G[AT]GC[AT]^C BamHI 345 GGATCC G^GATCC BanI 40 GGCACC G^G[CT][GA]CC BcgI 912 CGAATTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1195 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 769 AGATCT A^GATCT BlpI 399 GCTCAGC GC^T[ACGT]AGC BpmI 1084 CTGGAG CTGGAG(16/14) Bpu1102I 399 GCTCAGC GC^T[ACGT]AGC BsaAI 898 TACGTG [CT]AC^GT[GA] BsaI 416 GAGACC GGTCTC(1/5) BsaWI 740 TCCGGA [AT]^CCGG[AT] BseAI 740 TCCGGA T^CCGGA BsiHKAI 736 GTGCTC G[AT]GC[AT]^C BspEI 740 TCCGGA T^CCGGA BspMI 1031 ACCTGC ACCTGC(4/8) BsrDI 542 GCAATG GCAATG(2/0) BstXI 288 CCAGGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 399 GCTCAGC GC^T[ACGT]AGC EaeI 273 CGGCCA [CT]^GGCC[GA] EarI 580 CTCTTC CTCTTC(1/4) HaeII 103 AGCGCC [GA]GCGC^[CT] HgaI 718 GCGTC GACGC(5/10) HincII 208 GTCAAC GT[CT]^[GA]AC HindII 208 GTCAAC GT[CT]^[GA]AC Ksp632I 580 CTCTTC CTCTTC(1/4) MluI 716 ACGCGT A^CGCGT MroI 740 TCCGGA T^CCGGA MslI 701 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG Psp1406I 835 AACGTT AA^CGTT SbfI 811 CCTGCAGG CCTGCA^GG SfaNI 1094 GCATC GCATC(5/9) SfiI 1194 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 474 CCCGGG CCC^GGG SmlI 1198 CTCAAG C^T[CT][GA]AG SpeI 855 ACTAGT A^CTAGT Sse8387I 811 CCTGCAGG CCTGCA^GG XcmI 1073 CCAACCCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 474 CCCGGG C^CCGGG XmaI 474 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 913 GAATTT [GA]^AATT[CT] 937 GAATTT AflIII 716 ACGCGT A^C[GA][CT]GT 899 ACGTGT ApoI 913 GAATTT [GA]^AATT[CT] 937 GAATTT AvaI 474 CCCGGG C^[CT]CG[GA]G 703 CCCGAG BanII 298 GGGCTC G[GA]GC[CT]^C 1087 GAGCCC BbsI 469 GAAGAC GAAGAC(2/6) 610 GTCTTC BbvCI 642 CCTCAGC CCTCAGC(-5/-2) 1221 CCTCAGC BfaI 856 CTAG C^TAG 880 CTAG BpuAI 469 GAAGAC GAAGAC(2/6) 610 GTCTTC BsmBI 549 CGTCTC CGTCTC(1/5) 819 GAGACG BsoBI 474 CCCGGG C^[CT]CG[GA]G 703 CCCGAG BsrI 786 ACTGG ACTGG(1/-1) 183 CCAGT BssSI 99 CACGAG CACGAG(-5/-1) 370 CACGAG CfoI 104 GCGC GCG^C 397 GCGC EcoT14I 23 CCATGG C^C[AT][AT]GG 1205 CCATGG HhaI 104 GCGC GCG^C 397 GCGC HinP1I 104 GCGC G^CGC 397 GCGC HinfI 292 GAGTC G^A[ACGT]TC 410 GACTC KspI 1148 CCGCGG CCGC^GG 1160 CCGCGG MaeI 856 CTAG C^TAG 880 CTAG NcoI 23 CCATGG C^CATGG 1205 CCATGG NspI 462 GCATGT [GA]CATG^[CT] 989 ACATGC PleI 410 GACTC GAGTC(4/5) 292 GAGTC PpuMI 374 AGGACCT [GA]G^G[AT]CC[CT] 455 AGGTCCC PstI 812 CTGCAG CTGCA^G 1174 CTGCAG PvuII 70 CAGCTG CAG^CTG 331 CAGCTG SacII 1148 CCGCGG CCGC^GG 1160 CCGCGG StyI 23 CCATGG C^C[AT][AT]GG 1205 CCATGG TspRI 78 CACTG CA[GC]TG[ACGT][ACGT]^ 1266 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I XhoII