Restriction map for
pF1KB6706
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI ItaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MluI MroI MslI
MvaI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 336 AAATTC [GA]^AATT[CT]
AfaI 102 GTAC GT^AC
ApoI 336 AAATTC [GA]^AATT[CT]
AseI 198 ATTAAT AT^TAAT
AsnI 198 ATTAAT AT^TAAT
AvaII 92 GGTCC G^G[AT]CC
BalI 270 TGGCCA TGG^CCA
BanII 136 GAGCCC G[GA]GC[CT]^C
BbsI 380 GAAGAC GAAGAC(2/6)
BbvCI 181 CCTCAGC CCTCAGC(-5/-2)
BcnI 167 CCCGG CC^[GC]GG
BglII 275 AGATCT A^GATCT
BmyI 136 GAGCCC G[AGT]GC[ACT]^C
BpuAI 380 GAAGAC GAAGAC(2/6)
BsaI 171 GAGACC GGTCTC(1/5)
BsaJI 17 CCTTGG C^C[ACGT][ACGT]GG
Bsp1286I 136 GAGCCC G[AGT]GC[ACT]^C
BsrDI 361 GCAATG GCAATG(2/0)
BssKI 167 CCCGG ^CC[ACGT]GG
BssSI 10 CTCGTG CACGAG(-5/-1)
BstAPI 147 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 213 GGATG GGATG(2/0)
BstYI 275 AGATCT [GA]^GATC[CT]
DraI 333 TTTAAA TTT^AAA
EaeI 270 TGGCCA [CT]^GGCC[GA]
EcoNI 246 CCTGTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 17 CCTTGG C^C[AT][AT]GG
FokI 213 GGATG GGATG(9/13)
MaeII 384 ACGT A^CGT
MfeI 227 CAATTG C^AATTG
MflI 275 AGATCT [GA]^GATC[CT]
MluNI 270 TGGCCA TGG^CCA
MscI 270 TGGCCA TGG^CCA
MspA1I 46 CAGCTG C[AC]G^C[GT]G
MunI 227 CAATTG C^AATTG
NciI 167 CCCGG CC^[GC]GG
NlaIII 268 CATG CATG^
PshBI 198 ATTAAT AT^TAAT
PstI 154 CTGCAG CTGCA^G
PvuII 46 CAGCTG CAG^CTG
RsaI 102 GTAC GT^AC
ScrFI 167 CCCGG CC^[ACGT]GG
SfcI 154 CTGCAG C^T[GA][CT]AG
StyI 17 CCTTGG C^C[AT][AT]GG
XhoII 275 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 47 AGCT AG^CT
330 AGCT
BsiYI 114 CCAGATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
246 CCTGTTAAAGG
BslI 114 CCAGATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
246 CCTGTTAAAGG
BsmAI 171 GAGAC GTCTC(1/5)
37 GTCTC
BsrI 89 ACTGG ACTGG(1/-1)
98 ACTGG
Cfr13I 92 GGTCC G^G[ACGT]CC
243 GGCCC
DdeI 44 CTCAG C^T[ACGT]AG
182 CTCAG
HapII 133 CCGG C^CGG
168 CCGG
HpaII 133 CCGG C^CGG
168 CCGG
MspI 133 CCGG C^CGG
168 CCGG
MwoI 138 GCCCTTTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
147 GCATCTTCTGC
NlaIV 67 GGAGCC GG[ACGT]^[ACGT]CC
135 GGAGCC
Sau96I 92 GGTCC G^G[ACGT]CC
243 GGCCC
SfaNI 214 GATGC GCATC(5/9)
147 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
DpnII HaeIII HinfI MboI MboII
MnlI MseI NdeII Sau3AI TfiI
Tru9I Tsp509I