Restriction map for
pF1KE9527
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MluI
MroI MspI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 241 GTCTAC GT^[AC][GT]AC
AccII 15 CGCG CG^CG
AlwNI 773 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 70 GGCACC G^G[CT][GA]CC
BanII 6 GAGCCC G[GA]GC[CT]^C
BbsI 226 GTCTTC GAAGAC(2/6)
BciVI 1114 GTATCC GTATCC(6/5)
BclI 794 TGATCA T^GATCA
BglI 343 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 226 GTCTTC GAAGAC(2/6)
BseRI 364 CTCCTC GAGGAG(10/8)
BsgI 511 GTGCAG GTGCAG(16/14)
BsmI 800 GAATGC GAATGC(1/-1)
Bsp1407I 974 TGTACA T^GTACA
BspMI 499 ACCTGC ACCTGC(4/8)
BsrDI 567 CATTGC GCAATG(2/0)
BsrGI 974 TGTACA T^GTACA
BstAPI 562 GCAAACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 612 GGTCACC G^GT[ACGT]ACC
BstPI 612 GGTCACC G^GT[ACGT]ACC
BstUI 15 CGCG CG^CG
BstXI 138 CCACGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 91 AGATCC [GA]^GATC[CT]
Cac8I 45 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 14 GCGC GCG^C
DrdI 1018 GACCCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 612 GGTCACC G^GT[ACGT]ACC
FbaI 794 TGATCA T^GATCA
HhaI 14 GCGC GCG^C
HinP1I 14 GCGC G^CGC
HinfI 603 GATTC G^A[ACGT]TC
MaeII 140 ACGT A^CGT
MflI 91 AGATCC [GA]^GATC[CT]
MspA1I 1008 CAGCTG C[AC]G^C[GT]G
MvnI 15 CGCG CG^CG
NheI 45 GCTAGC G^CTAGC
PshAI 219 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 63 CTGCAG CTGCA^G
PvuII 1008 CAGCTG CAG^CTG
SfcI 63 CTGCAG C^T[GA][CT]AG
SfiI 342 GGCCAATCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 974 TGTACA T^GTACA
TaqI 1016 TCGA T^CGA
TfiI 603 GATTC G^A[AT]TC
TthHB8I 1016 TCGA T^CGA
XcmI 344 CCAATCTGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 91 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 473 ACATGT A^C[GA][CT]GT
713 ACATGT
AlwI 92 GATCC GGATC(4/5)
1056 GGATC
BfaI 46 CTAG C^TAG
905 CTAG
BmyI 6 GAGCCC G[AGT]GC[ACT]^C
544 GGGCAC
BpmI 549 CTCCAG CTGGAG(16/14)
1137 CTCCAG
BsmAI 710 GAGAC GTCTC(1/5)
868 GTCTC
Bsp1286I 6 GAGCCC G[AGT]GC[ACT]^C
544 GGGCAC
BspHI 494 TCATGA T^CATGA
678 TCATGA
BspLU11I 473 ACATGT A^CATGT
713 ACATGT
DsaI 539 CCATGG C^C[GA][CT]GG
818 CCATGG
HphI 614 TCACC GGTGA(8/7)
1121 TCACC
MaeI 46 CTAG C^TAG
905 CTAG
MfeI 236 CAATTG C^AATTG
248 CAATTG
MunI 236 CAATTG C^AATTG
248 CAATTG
NcoI 539 CCATGG C^CATGG
818 CCATGG
NspI 473 ACATGT [GA]CATG^[CT]
713 ACATGT
RcaI 494 TCATGA T^CATGA
678 TCATGA
Tsp45I 142 GTCAC ^GT[GC]AC
613 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII ItaI Ksp632I MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp509I TspRI