################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 307 GTAGAC GT^[AC][GT]AC AocI 771 CCTGAGG CC^T[ACGT]AGG ApaI 4 GGGCCC GGGCC^C Asp700I 156 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 754 GAGCAC G[AT]GC[AT]^C AvaI 205 CCCGAG C^[CT]CG[GA]G BalI 332 TGGCCA TGG^CCA BanII 4 GGGCCC G[GA]GC[CT]^C BcgI 408 CGAGTTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 128 TGATCA T^GATCA BcnI 19 CCGGG CC^[GC]GG BglII 924 AGATCT A^GATCT BsaI 802 GAGACC GGTCTC(1/5) BsiHKAI 754 GAGCAC G[AT]GC[AT]^C BsoBI 205 CCCGAG C^[CT]CG[GA]G BspMI 1079 ACCTGC ACCTGC(4/8) BstYI 924 AGATCT [GA]^GATC[CT] Bsu36I 771 CCTGAGG CC^T[ACGT]AGG DraIII 832 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 60 GACACCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 332 TGGCCA [CT]^GGCC[GA] Eco81I 771 CCTGAGG CC^T[ACGT]AGG FbaI 128 TGATCA T^GATCA HincII 739 GTCAAC GT[CT]^[GA]AC HindII 739 GTCAAC GT[CT]^[GA]AC HphI 478 GGTGA GGTGA(8/7) MaeII 988 ACGT A^CGT MflI 924 AGATCT [GA]^GATC[CT] MluNI 332 TGGCCA TGG^CCA MscI 332 TGGCCA TGG^CCA MslI 423 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 19 CCGGG CC^[GC]GG NspI 365 GCATGT [GA]CATG^[CT] PflMI 831 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 490 CTGCAG CTGCA^G SapI 691 GAAGAGC GCTCTTC(1/4) SfcI 490 CTGCAG C^T[GA][CT]AG SmlI 889 CTCAAG C^T[CT][GA]AG SspI 505 AATATT AAT^ATT TfiI 827 GAATC G^A[AT]TC Tsp45I 700 GTGAC ^GT[GC]AC Van91I 831 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 924 AGATCT [GA]^GATC[CT] XmnI 156 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 27 GCGG CCGC(-3/-1) 1042 GCGG AlwI 252 GATCC GGATC(4/5) 859 GATCC BbsI 280 GAAGAC GAAGAC(2/6) 984 GAAGAC BbvCI 583 GCTGAGG CCTCAGC(-5/-2) 1090 GCTGAGG BmyI 4 GGGCCC G[AGT]GC[ACT]^C 754 GAGCAC BpuAI 280 GAAGAC GAAGAC(2/6) 984 GAAGAC BsaJI 206 CCGAGG C^C[ACGT][ACGT]GG 806 CCTGGG BsmAI 802 GAGAC GTCTC(1/5) 883 GAGAC BsmFI 58 GGGAC GGGAC(10/14) 970 GTCCC Bsp1286I 4 GGGCCC G[AGT]GC[ACT]^C 754 GAGCAC BsrI 977 ACTGG ACTGG(1/-1) 1106 ACTGG EarI 691 GAAGAG CTCTTC(1/4) 727 GAAGAG HapII 19 CCGG C^CGG 787 CCGG HpaII 19 CCGG C^CGG 787 CCGG Ksp632I 691 GAAGAG CTCTTC(1/4) 727 GAAGAG MaeIII 700 GTGAC ^GT[ACGT]AC 1033 GTTAC MspA1I 326 CAGCTG C[AC]G^C[GT]G 581 CAGCTG MspI 19 CCGG C^CGG 787 CCGG PpuMI 455 AGGACCC [GA]G^G[AT]CC[CT] 1076 AGGACCT PvuII 326 CAGCTG CAG^CTG 581 CAGCTG Tsp509I 233 AATT ^AATT 467 AATT TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^ 912 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AfaI AluI AvaII BbvI BfaI BpmI BseRI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StuI TaqI TseI TthHB8I