Restriction map for
pF1KB9903
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsgI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 89 AAATTC [GA]^AATT[CT]
Alw44I 500 GTGCAC G^TGCAC
AlwI 684 GATCC GGATC(4/5)
ApaI 709 GGGCCC GGGCC^C
ApaLI 500 GTGCAC G^TGCAC
ApoI 89 AAATTC [GA]^AATT[CT]
AspHI 500 GTGCAC G[AT]GC[AT]^C
AspI 745 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 709 GGGCCC G[GA]GC[CT]^C
BbvI 669 GCTGC GCAGC(8/12)
BciVI 356 GTATCC GTATCC(6/5)
BclI 249 TGATCA T^GATCA
BfaI 109 CTAG C^TAG
BpmI 590 CTCCAG CTGGAG(16/14)
BsaWI 369 TCCGGT [AT]^CCGG[AT]
BsiEI 60 CGACCG CG[GA][CT]^CG
BsiHKAI 500 GTGCAC G[AT]GC[AT]^C
BsmAI 296 GTCTC GTCTC(1/5)
BsmFI 243 GGGAC GGGAC(10/14)
BsmI 615 GAATGC GAATGC(1/-1)
BssSI 298 CTCGTG CACGAG(-5/-1)
BstAPI 502 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 372 GGTAACC G^GT[ACGT]ACC
BstPI 372 GGTAACC G^GT[ACGT]ACC
BstYI 683 AGATCC [GA]^GATC[CT]
DraII 708 AGGGCCC [GA]G^G[ACGT]CC[CT]
EarI 730 GAAGAG CTCTTC(1/4)
EcoO109I 708 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 372 GGTAACC G^GT[ACGT]ACC
FbaI 249 TGATCA T^GATCA
HgaI 462 GCGTC GACGC(5/10)
Ksp632I 730 GAAGAG CTCTTC(1/4)
MaeI 109 CTAG C^TAG
MaeII 118 ACGT A^CGT
MflI 683 AGATCC [GA]^GATC[CT]
MseI 542 TTAA T^TAA
PflFI 745 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 547 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 670 CTGCAG CTGCA^G
ScaI 429 AGTACT AGT^ACT
SfcI 670 CTGCAG C^T[GA][CT]AG
TaqI 26 TCGA T^CGA
Tru9I 542 TTAA T^TAA
TseI 669 GCTGC G^C[AT]GC
Tsp45I 751 GTCAC ^GT[GC]AC
Tsp509I 90 AATT ^AATT
TspRI 714 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 745 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 26 TCGA T^CGA
Van91I 547 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 683 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 51 CGCG CG^CG
58 CGCG
AfaI 42 GTAC GT^AC
430 GTAC
AlwNI 253 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
548 CAGGTGCTG
Asp700I 274 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
477 GAAAATGTTC
BbsI 191 GAAGAC GAAGAC(2/6)
742 GAAGAC
BcnI 63 CCGGG CC^[GC]GG
78 CCGGG
BmyI 500 GTGCAC G[AGT]GC[ACT]^C
709 GGGCCC
BpuAI 191 GAAGAC GAAGAC(2/6)
742 GAAGAC
Bsp1286I 500 GTGCAC G[AGT]GC[ACT]^C
709 GGGCCC
BstF5I 171 GGATG GGATG(2/0)
597 GGATG
BstUI 51 CGCG CG^CG
58 CGCG
Cac8I 30 GCAGGC GC[ACGT]^[ACGT]GC
507 GCTTGC
CfoI 37 GCGC GCG^C
57 GCGC
DdeI 352 CTAAG C^T[ACGT]AG
361 CTAAG
Fnu4HI 34 GCGGC GC^[ACGT]GC
669 GCTGC
FokI 171 GGATG GGATG(9/13)
597 GGATG
HaeIII 584 GGCC GG^CC
710 GGCC
HhaI 37 GCGC GCG^C
57 GCGC
HinP1I 37 GCGC G^CGC
57 GCGC
ItaI 34 GCGGC GC^[ACGT]GC
669 GCTGC
MaeIII 373 GTAAC ^GT[ACGT]AC
751 GTCAC
MvnI 51 CGCG CG^CG
58 CGCG
NciI 63 CCGGG CC^[GC]GG
78 CCGGG
RsaI 42 GTAC GT^AC
430 GTAC
SfaNI 133 GATGC GCATC(5/9)
328 GCATC
XmnI 274 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
477 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AvaII BsaJI BsiYI
BslI BsrI BssKI BstNI Cfr13I
DpnII EcoRII HapII HpaII HphI
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI