Restriction map for
pF1KB3236
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AvaII AviII BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BcnI BfrI BglI BglII BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I Bsu36I Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV FauI FseI FspI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MluI MroI MspA1I MspI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuII RcaI RsrII SacI
SacII SalI Sau96I SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 40 CGCG CG^CG
AfaI 1637 GTAC GT^AC
AlwNI 818 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1014 ATTAAT AT^TAAT
AsnI 1014 ATTAAT AT^TAAT
Asp700I 1162 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 918 CCTAGG C^CTAGG
BanII 4 GAGCCC G[GA]GC[CT]^C
BbvCI 1444 GCTGAGG CCTCAGC(-5/-2)
BclI 879 TGATCA T^GATCA
BlnI 918 CCTAGG C^CTAGG
BlpI 1456 GCTCAGC GC^T[ACGT]AGC
BmyI 4 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 1456 GCTCAGC GC^T[ACGT]AGC
BseRI 598 GAGGAG GAGGAG(10/8)
BsgI 1687 GTGCAG GTGCAG(16/14)
BsiEI 384 CGATCG CG[GA][CT]^CG
BsmBI 760 CGTCTC CGTCTC(1/5)
Bsp1286I 4 GAGCCC G[AGT]GC[ACT]^C
BsrBI 1620 CCGCTC CCGCTC(-3/-3)
BstBI 854 TTCGAA TT^CGAA
BstUI 40 CGCG CG^CG
BstXI 897 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1456 GCTCAGC GC^T[ACGT]AGC
CfoI 39 GCGC GCG^C
EcoRI 679 GAATTC G^AATTC
FbaI 879 TGATCA T^GATCA
HhaI 39 GCGC GCG^C
HinP1I 39 GCGC G^CGC
HincII 1708 GTTGAC GT[CT]^[GA]AC
HindII 1708 GTTGAC GT[CT]^[GA]AC
MvnI 40 CGCG CG^CG
NspV 854 TTCGAA TT^CGAA
PshBI 1014 ATTAAT AT^TAAT
PstI 1247 CTGCAG CTGCA^G
PvuI 384 CGATCG CGAT^CG
RsaI 1637 GTAC GT^AC
SapI 850 GCTCTTC GCTCTTC(1/4)
ScaI 1636 AGTACT AGT^ACT
SfcI 1247 CTGCAG C^T[GA][CT]AG
SfuI 854 TTCGAA TT^CGAA
SmlI 554 CTCAAG C^T[CT][GA]AG
SspI 1426 AATATT AAT^ATT
XcmI 311 CCACTGTGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1162 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BalI 418 TGGCCA TGG^CCA
1347 TGGCCA
BpmI 905 CTGGAG CTGGAG(16/14)
1376 CTGGAG
BsmFI 1026 GGGAC GGGAC(10/14)
1275 GGGAC
BsmI 750 GAATGC GAATGC(1/-1)
1153 GCATTC
BspMI 47 ACCTGC ACCTGC(4/8)
835 GCAGGT
Cac8I 325 GCTTGC GC[ACGT]^[ACGT]GC
535 GCTTGC
EaeI 418 TGGCCA [CT]^GGCC[GA]
1347 TGGCCA
EcoNI 69 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1345 CCTGGCCAAGG
HphI 447 GGTGA GGTGA(8/7)
66 TCACC
MaeII 1029 ACGT A^CGT
1278 ACGT
MfeI 109 CAATTG C^AATTG
1142 CAATTG
MluNI 418 TGGCCA TGG^CCA
1347 TGGCCA
MscI 418 TGGCCA TGG^CCA
1347 TGGCCA
MunI 109 CAATTG C^AATTG
1142 CAATTG
Tsp45I 448 GTGAC ^GT[GC]AC
1315 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BfaI BsaI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstAPI
BstF5I BstNI BstYI DdeI DpnII
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NsiI Sau3AI
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII