Restriction map for
pF1KE6704
##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccI AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AviII BalI BclI BfrI BsaBI
BsiEI BsiWI BspDI BspHI BspLU11I
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EaeI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluNI MscI MunI
NcoI NdeI NotI NruI NsiI
NspV PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 1442 TCCGGA T^CCGGA
AflIII 1643 ACGCGT A^C[GA][CT]GT
AgeI 464 ACCGGT A^CCGGT
Alw44I 2127 GTGCAC G^TGCAC
ApaI 1343 GGGCCC GGGCC^C
ApaLI 2127 GTGCAC G^TGCAC
AspI 391 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1781 GGATCC G^GATCC
BbeI 1999 GGCGCC GGCGC^C
BbrPI 2733 CACGTG CAC^GTG
BcgI 3046 CGACTGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 288 GGATAC GTATCC(6/5)
BglII 2591 AGATCT A^GATCT
BseAI 1442 TCCGGA T^CCGGA
BsmBI 1470 CGTCTC CGTCTC(1/5)
BsmI 781 GAATGC GAATGC(1/-1)
Bsp1407I 1301 TGTACA T^GTACA
BspEI 1442 TCCGGA T^CCGGA
BsrBI 232 GAGCGG CCGCTC(-3/-3)
BsrGI 1301 TGTACA T^GTACA
DrdI 3034 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1555 GAATTC G^AATTC
HgaI 2095 GACGC GACGC(5/10)
KasI 1999 GGCGCC G^GCGCC
MluI 1643 ACGCGT A^CGCGT
MroI 1442 TCCGGA T^CCGGA
NaeI 2768 GCCGGC GCC^GGC
NarI 1999 GGCGCC GG^CGCC
NgoMIV 2768 GCCGGC G^CCGGC
NheI 571 GCTAGC G^CTAGC
PaeR7I 58 CTCGAG C^TCGAG
PflFI 391 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 295 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 464 ACCGGT A^CCGGT
PmaCI 2733 CACGTG CAC^GTG
PmlI 2733 CACGTG CAC^GTG
PshAI 1274 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 714 GCTCTTC GCTCTTC(1/4)
SfoI 1999 GGCGCC GGC^GCC
SphI 788 GCATGC GCATG^C
SspBI 1301 TGTACA T^GTACA
StuI 203 AGGCCT AGG^CCT
Tth111I 391 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 295 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 58 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 1278 GACGTC GACGT^C
1468 GACGTC
Aor51HI 1964 AGCGCT AGC^GCT
3144 AGCGCT
AvrII 438 CCTAGG C^CTAGG
3027 CCTAGG
BbvCI 456 CCTCAGC CCTCAGC(-5/-2)
2884 GCTGAGG
BglI 2768 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3068 GCCATCCTGGC
BlnI 438 CCTAGG C^CTAGG
3027 CCTAGG
BlpI 2267 GCTCAGC GC^T[ACGT]AGC
3063 GCTGAGC
Bpu1102I 2267 GCTCAGC GC^T[ACGT]AGC
3063 GCTGAGC
BsaAI 1168 TACGTG [CT]AC^GT[GA]
2733 CACGTG
BsaWI 464 ACCGGT [AT]^CCGG[AT]
1442 TCCGGA
BsrDI 1920 CATTGC GCAATG(2/0)
428 GCAATG
BssHII 433 GCGCGC G^CGCGC
827 GCGCGC
CelII 2267 GCTCAGC GC^T[ACGT]AGC
3063 GCTGAGC
Eco47III 1964 AGCGCT AGC^GCT
3144 AGCGCT
MseI 971 TTAA T^TAA
979 TTAA
SacI 841 GAGCTC GAGCT^C
892 GAGCTC
SexAI 278 ACCTGGT A^CC[AT]GGT
297 ACCTGGT
Tru9I 971 TTAA T^TAA
979 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI