################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaI BseAI BseRI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflIII 348 ACGTGT A^C[GA][CT]GT AgeI 34 ACCGGT A^CCGGT AlwNI 205 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG BbsI 316 GAAGAC GAAGAC(2/6) BbvI 262 GCAGC GCAGC(8/12) BfaI 379 CTAG C^TAG BglII 243 AGATCT A^GATCT BpmI 98 CTGGAG CTGGAG(16/14) BpuAI 316 GAAGAC GAAGAC(2/6) BsaAI 392 TACGTG [CT]AC^GT[GA] BsaJI 49 CCCAGG C^C[ACGT][ACGT]GG BsaWI 34 ACCGGT [AT]^CCGG[AT] BsiEI 32 CGACCG CG[GA][CT]^CG BsrBI 74 CCGCTC CCGCTC(-3/-3) BsrFI 34 ACCGGT [GA]^CCGG[CT] BstF5I 372 CATCC GGATG(2/0) BstYI 243 AGATCT [GA]^GATC[CT] Cfr10I 34 ACCGGT [GA]^CCGG[CT] DdeI 227 CTCAG C^T[ACGT]AG DpnII 244 GATC ^GATC DraI 235 TTTAAA TTT^AAA FauI 23 CCCGC CCCGC(4/6) FokI 372 CATCC GGATG(9/13) HaeII 141 GGCGCT [GA]GCGC^[CT] HapII 35 CCGG C^CGG HgaI 207 GCGTC GACGC(5/10) HinfI 186 GATTC G^A[ACGT]TC HpaII 35 CCGG C^CGG HphI 105 GGTGA GGTGA(8/7) MaeI 379 CTAG C^TAG MboI 244 GATC ^GATC MflI 243 AGATCT [GA]^GATC[CT] MseI 236 TTAA T^TAA MspI 35 CCGG C^CGG NdeII 244 GATC ^GATC NheI 378 GCTAGC G^CTAGC NlaIII 340 CATG CATG^ PflMI 204 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 34 ACCGGT A^CCGGT Sau3AI 244 GATC ^GATC TaqI 31 TCGA T^CGA TfiI 186 GATTC G^A[AT]TC Tru9I 236 TTAA T^TAA TseI 262 GCAGC G^C[AT]GC TspRI 174 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 31 TCGA T^CGA Van91I 204 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 50 CCAGGAAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 243 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 8 GTAC GT^AC 134 GTAC AluI 264 AGCT AG^CT 377 AGCT BsiYI 91 CCCTCTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 204 CCAGCGTCTGG BslI 91 CCCTCTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 204 CCAGCGTCTGG BsrI 60 ACTGG ACTGG(1/-1) 97 ACTGG BssKI 50 CCAGG ^CC[ACGT]GG 130 CCTGG BstNI 50 CCAGG CC^[AT]GG 130 CCTGG EcoRII 50 CCAGG ^CC[AT]GG 130 CCTGG MaeIII 122 GTTAC ^GT[ACGT]AC 352 GTAAC MboII 108 GAAGA GAAGA(8/7) 316 GAAGA MvaI 50 CCAGG CC^[AT]GG 130 CCTGG RsaI 8 GTAC GT^AC 134 GTAC ScrFI 50 CCAGG CC^[ACGT]GG 130 CCTGG StuI 127 AGGCCT AGG^CCT 293 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AciI Cac8I CfoI Fnu4HI HaeIII HhaI HinP1I ItaI MaeII MnlI MwoI Tsp509I