Restriction map for
pF1KB8486
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII BamHI BbeI BclI
BfrI BglII BsaBI BsiWI BsmI
BspHI BspLU11I Bst1107I BstBI BstZ17I
Bsu36I CpoI DraI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI MamI MfeI
MluI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I RcaI RsrII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SpeI Sse8387I SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 1394 TCCGGA T^CCGGA
AhdI 790 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 487 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 790 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1447 GAGCTC G[AT]GC[AT]^C
AvrII 1003 CCTAGG C^CTAGG
BalI 347 TGGCCA TGG^CCA
BbvCI 2379 CCTCAGC CCTCAGC(-5/-2)
BcgI 446 GCAGCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1391 GTATCC GTATCC(6/5)
BfaI 1004 CTAG C^TAG
BglI 2221 GCCGGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1003 CCTAGG C^CTAGG
BlpI 765 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 765 GCTGAGC GC^T[ACGT]AGC
BsaI 1953 GGTCTC GGTCTC(1/5)
BsaWI 1394 TCCGGA [AT]^CCGG[AT]
BseAI 1394 TCCGGA T^CCGGA
BsiEI 9 CGATCG CG[GA][CT]^CG
BsiHKAI 1447 GAGCTC G[AT]GC[AT]^C
BsmBI 2480 GAGACG CGTCTC(1/5)
BspDI 1975 ATCGAT AT^CGAT
BspEI 1394 TCCGGA T^CCGGA
BsrDI 1631 GCAATG GCAATG(2/0)
BstAPI 2360 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 576 GGTCACC G^GT[ACGT]ACC
BstPI 576 GGTCACC G^GT[ACGT]ACC
BstXI 1943 CCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 765 GCTGAGC GC^T[ACGT]AGC
ClaI 1975 ATCGAT AT^CGAT
DrdI 247 GACGTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 790 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1510 CTCTTC CTCTTC(1/4)
EcoO65I 576 GGTCACC G^GT[ACGT]ACC
HincII 121 GTCAAC GT[CT]^[GA]AC
HindII 121 GTCAAC GT[CT]^[GA]AC
Ksp632I 1510 CTCTTC CTCTTC(1/4)
MaeI 1004 CTAG C^TAG
MluNI 347 TGGCCA TGG^CCA
MroI 1394 TCCGGA T^CCGGA
MscI 347 TGGCCA TGG^CCA
PshAI 2420 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 9 CGATCG CGAT^CG
SacI 1447 GAGCTC GAGCT^C
SnaBI 1031 TACGTA TAC^GTA
SphI 1896 GCATGC GCATG^C
StuI 1667 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcsI 297 GAATTT [GA]^AATT[CT]
1147 AAATTC
ApoI 297 GAATTT [GA]^AATT[CT]
1147 AAATTC
AvaI 153 CCCGGG C^[CT]CG[GA]G
1359 CCCGGG
BbrPI 1371 CACGTG CAC^GTG
1482 CACGTG
BbsI 265 GTCTTC GAAGAC(2/6)
2212 GTCTTC
BpuAI 265 GTCTTC GAAGAC(2/6)
2212 GTCTTC
BsoBI 153 CCCGGG C^[CT]CG[GA]G
1359 CCCGGG
BsrBI 716 CCGCTC CCGCTC(-3/-3)
961 GAGCGG
BssHII 553 GCGCGC G^CGCGC
2276 GCGCGC
BstYI 2300 AGATCC [GA]^GATC[CT]
2450 GGATCT
DraIII 490 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2007 CACGCAGTG
EcoNI 492 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2286 CCTGCAAAAGG
HaeII 158 GGCGCT [GA]GCGC^[CT]
2053 AGCGCC
KspI 547 CCGCGG CCGC^GG
660 CCGCGG
MflI 2300 AGATCC [GA]^GATC[CT]
2450 GGATCT
NaeI 1468 GCCGGC GCC^GGC
2221 GCCGGC
NcoI 851 CCATGG C^CATGG
2174 CCATGG
NgoMIV 1468 GCCGGC G^CCGGC
2221 GCCGGC
NspI 1128 GCATGT [GA]CATG^[CT]
1896 GCATGC
PflMI 861 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2006 CCACGCAGTGG
PmaCI 1371 CACGTG CAC^GTG
1482 CACGTG
PmlI 1371 CACGTG CAC^GTG
1482 CACGTG
PstI 639 CTGCAG CTGCA^G
1455 CTGCAG
SacII 547 CCGCGG CCGC^GG
660 CCGCGG
SexAI 491 ACCTGGT A^CC[AT]GGT
821 ACCTGGT
SmaI 153 CCCGGG CCC^GGG
1359 CCCGGG
TfiI 1988 GATTC G^A[AT]TC
2410 GATTC
Tsp509I 298 AATT ^AATT
1148 AATT
Van91I 861 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2006 CCACGCAGTGG
XcmI 1193 CCAAGGACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1623 CCAGCAGTGCAATGG
XhoII 2300 AGATCC [GA]^GATC[CT]
2450 GGATCT
XmaCI 153 CCCGGG C^CCGGG
1359 CCCGGG
XmaI 153 CCCGGG C^CCGGG
1359 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI Asp700I AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TseI Tsp45I
TspRI TthHB8I XmnI