Restriction map for pF1KB8486
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AviII       BamHI       BbeI        BclI        
BfrI        BglII       BsaBI       BsiWI       BsmI        
BspHI       BspLU11I    Bst1107I    BstBI       BstZ17I     
Bsu36I      CpoI        DraI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        MamI        MfeI        
MluI        MseI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SpeI        Sse8387I    SspI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII       1394  TCCGGA               T^CCGGA
AhdI          790  GACCTTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         487  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         790  GACCTTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1447  GAGCTC               G[AT]GC[AT]^C
AvrII        1003  CCTAGG               C^CTAGG
BalI          347  TGGCCA               TGG^CCA
BbvCI        2379  CCTCAGC              CCTCAGC(-5/-2)
BcgI          446  GCAGCGTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1391  GTATCC               GTATCC(6/5)
BfaI         1004  CTAG                 C^TAG
BglI         2221  GCCGGCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1003  CCTAGG               C^CTAGG
BlpI          765  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      765  GCTGAGC              GC^T[ACGT]AGC
BsaI         1953  GGTCTC               GGTCTC(1/5)
BsaWI        1394  TCCGGA               [AT]^CCGG[AT]
BseAI        1394  TCCGGA               T^CCGGA
BsiEI           9  CGATCG               CG[GA][CT]^CG
BsiHKAI      1447  GAGCTC               G[AT]GC[AT]^C
BsmBI        2480  GAGACG               CGTCTC(1/5)
BspDI        1975  ATCGAT               AT^CGAT
BspEI        1394  TCCGGA               T^CCGGA
BsrDI        1631  GCAATG               GCAATG(2/0)
BstAPI       2360  GCATGATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        576  GGTCACC              G^GT[ACGT]ACC
BstPI         576  GGTCACC              G^GT[ACGT]ACC
BstXI        1943  CCAACTGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         765  GCTGAGC              GC^T[ACGT]AGC
ClaI         1975  ATCGAT               AT^CGAT
DrdI          247  GACGTGCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      790  GACCTTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1510  CTCTTC               CTCTTC(1/4)
EcoO65I       576  GGTCACC              G^GT[ACGT]ACC
HincII        121  GTCAAC               GT[CT]^[GA]AC
HindII        121  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1510  CTCTTC               CTCTTC(1/4)
MaeI         1004  CTAG                 C^TAG
MluNI         347  TGGCCA               TGG^CCA
MroI         1394  TCCGGA               T^CCGGA
MscI          347  TGGCCA               TGG^CCA
PshAI        2420  GACCCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI            9  CGATCG               CGAT^CG
SacI         1447  GAGCTC               GAGCT^C
SnaBI        1031  TACGTA               TAC^GTA
SphI         1896  GCATGC               GCATG^C
StuI         1667  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          297  GAATTT               [GA]^AATT[CT]
             1147  AAATTC              
ApoI          297  GAATTT               [GA]^AATT[CT]
             1147  AAATTC              
AvaI          153  CCCGGG               C^[CT]CG[GA]G
             1359  CCCGGG              
BbrPI        1371  CACGTG               CAC^GTG
             1482  CACGTG              
BbsI          265  GTCTTC               GAAGAC(2/6)
             2212  GTCTTC              
BpuAI         265  GTCTTC               GAAGAC(2/6)
             2212  GTCTTC              
BsoBI         153  CCCGGG               C^[CT]CG[GA]G
             1359  CCCGGG              
BsrBI         716  CCGCTC               CCGCTC(-3/-3)
              961  GAGCGG              
BssHII        553  GCGCGC               G^CGCGC
             2276  GCGCGC              
BstYI        2300  AGATCC               [GA]^GATC[CT]
             2450  GGATCT              
DraIII        490  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2007  CACGCAGTG           
EcoNI         492  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2286  CCTGCAAAAGG         
HaeII         158  GGCGCT               [GA]GCGC^[CT]
             2053  AGCGCC              
KspI          547  CCGCGG               CCGC^GG
              660  CCGCGG              
MflI         2300  AGATCC               [GA]^GATC[CT]
             2450  GGATCT              
NaeI         1468  GCCGGC               GCC^GGC
             2221  GCCGGC              
NcoI          851  CCATGG               C^CATGG
             2174  CCATGG              
NgoMIV       1468  GCCGGC               G^CCGGC
             2221  GCCGGC              
NspI         1128  GCATGT               [GA]CATG^[CT]
             1896  GCATGC              
PflMI         861  CCACGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2006  CCACGCAGTGG         
PmaCI        1371  CACGTG               CAC^GTG
             1482  CACGTG              
PmlI         1371  CACGTG               CAC^GTG
             1482  CACGTG              
PstI          639  CTGCAG               CTGCA^G
             1455  CTGCAG              
SacII         547  CCGCGG               CCGC^GG
              660  CCGCGG              
SexAI         491  ACCTGGT              A^CC[AT]GGT
              821  ACCTGGT             
SmaI          153  CCCGGG               CCC^GGG
             1359  CCCGGG              
TfiI         1988  GATTC                G^A[AT]TC
             2410  GATTC               
Tsp509I       298  AATT                 ^AATT
             1148  AATT                
Van91I        861  CCACGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2006  CCACGCAGTGG         
XcmI         1193  CCAAGGACTACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1623  CCAGCAGTGCAATGG     
XhoII        2300  AGATCC               [GA]^GATC[CT]
             2450  GGATCT              
XmaCI         153  CCCGGG               C^CCGGG
             1359  CCCGGG              
XmaI          153  CCCGGG               C^CCGGG
             1359  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        Asp700I     AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaAI       BsaHI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspMI       BsrFI       BsrGI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com