################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AviII BamHI BbeI BclI BfrI BglII BsaBI BsiWI BsmI BspHI BspLU11I Bst1107I BstBI BstZ17I Bsu36I CpoI DraI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI MamI MfeI MluI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I RcaI RsrII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SpeI Sse8387I SspI SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 1394 TCCGGA T^CCGGA AhdI 790 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 487 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 790 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1447 GAGCTC G[AT]GC[AT]^C AvrII 1003 CCTAGG C^CTAGG BalI 347 TGGCCA TGG^CCA BbvCI 2379 CCTCAGC CCTCAGC(-5/-2) BcgI 446 GCAGCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1391 GTATCC GTATCC(6/5) BfaI 1004 CTAG C^TAG BglI 2221 GCCGGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1003 CCTAGG C^CTAGG BlpI 765 GCTGAGC GC^T[ACGT]AGC Bpu1102I 765 GCTGAGC GC^T[ACGT]AGC BsaI 1953 GGTCTC GGTCTC(1/5) BsaWI 1394 TCCGGA [AT]^CCGG[AT] BseAI 1394 TCCGGA T^CCGGA BsiEI 9 CGATCG CG[GA][CT]^CG BsiHKAI 1447 GAGCTC G[AT]GC[AT]^C BsmBI 2480 GAGACG CGTCTC(1/5) BspDI 1975 ATCGAT AT^CGAT BspEI 1394 TCCGGA T^CCGGA BsrDI 1631 GCAATG GCAATG(2/0) BstAPI 2360 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 576 GGTCACC G^GT[ACGT]ACC BstPI 576 GGTCACC G^GT[ACGT]ACC BstXI 1943 CCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 765 GCTGAGC GC^T[ACGT]AGC ClaI 1975 ATCGAT AT^CGAT DrdI 247 GACGTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 790 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1510 CTCTTC CTCTTC(1/4) EcoO65I 576 GGTCACC G^GT[ACGT]ACC HincII 121 GTCAAC GT[CT]^[GA]AC HindII 121 GTCAAC GT[CT]^[GA]AC Ksp632I 1510 CTCTTC CTCTTC(1/4) MaeI 1004 CTAG C^TAG MluNI 347 TGGCCA TGG^CCA MroI 1394 TCCGGA T^CCGGA MscI 347 TGGCCA TGG^CCA PshAI 2420 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 9 CGATCG CGAT^CG SacI 1447 GAGCTC GAGCT^C SnaBI 1031 TACGTA TAC^GTA SphI 1896 GCATGC GCATG^C StuI 1667 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcsI 297 GAATTT [GA]^AATT[CT] 1147 AAATTC ApoI 297 GAATTT [GA]^AATT[CT] 1147 AAATTC AvaI 153 CCCGGG C^[CT]CG[GA]G 1359 CCCGGG BbrPI 1371 CACGTG CAC^GTG 1482 CACGTG BbsI 265 GTCTTC GAAGAC(2/6) 2212 GTCTTC BpuAI 265 GTCTTC GAAGAC(2/6) 2212 GTCTTC BsoBI 153 CCCGGG C^[CT]CG[GA]G 1359 CCCGGG BsrBI 716 CCGCTC CCGCTC(-3/-3) 961 GAGCGG BssHII 553 GCGCGC G^CGCGC 2276 GCGCGC BstYI 2300 AGATCC [GA]^GATC[CT] 2450 GGATCT DraIII 490 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 2007 CACGCAGTG EcoNI 492 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2286 CCTGCAAAAGG HaeII 158 GGCGCT [GA]GCGC^[CT] 2053 AGCGCC KspI 547 CCGCGG CCGC^GG 660 CCGCGG MflI 2300 AGATCC [GA]^GATC[CT] 2450 GGATCT NaeI 1468 GCCGGC GCC^GGC 2221 GCCGGC NcoI 851 CCATGG C^CATGG 2174 CCATGG NgoMIV 1468 GCCGGC G^CCGGC 2221 GCCGGC NspI 1128 GCATGT [GA]CATG^[CT] 1896 GCATGC PflMI 861 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2006 CCACGCAGTGG PmaCI 1371 CACGTG CAC^GTG 1482 CACGTG PmlI 1371 CACGTG CAC^GTG 1482 CACGTG PstI 639 CTGCAG CTGCA^G 1455 CTGCAG SacII 547 CCGCGG CCGC^GG 660 CCGCGG SexAI 491 ACCTGGT A^CC[AT]GGT 821 ACCTGGT SmaI 153 CCCGGG CCC^GGG 1359 CCCGGG TfiI 1988 GATTC G^A[AT]TC 2410 GATTC Tsp509I 298 AATT ^AATT 1148 AATT Van91I 861 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2006 CCACGCAGTGG XcmI 1193 CCAAGGACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1623 CCAGCAGTGCAATGG XhoII 2300 AGATCC [GA]^GATC[CT] 2450 GGATCT XmaCI 153 CCCGGG C^CCGGG 1359 CCCGGG XmaI 153 CCCGGG C^CCGGG 1359 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI Asp700I AvaII BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TseI Tsp45I TspRI TthHB8I XmnI