Restriction map for
pF1KB3060
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccII AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbeI BcgI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaHI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FseI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MaeI MfeI MluI
MunI MvnI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 29 TCCGGA T^CCGGA
AclI 1765 AACGTT AA^CGTT
Asp700I 1365 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1175 CCCGGG C^[CT]CG[GA]G
AviII 329 TGCGCA TGC^GCA
BalI 1793 TGGCCA TGG^CCA
BamHI 1082 GGATCC G^GATCC
BbrPI 1359 CACGTG CAC^GTG
BbvCI 481 GCTGAGG CCTCAGC(-5/-2)
BciVI 900 GTATCC GTATCC(6/5)
BclI 1869 TGATCA T^GATCA
BsaBI 441 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1024 GAGACC GGTCTC(1/5)
BseAI 29 TCCGGA T^CCGGA
BseRI 1267 CTCCTC GAGGAG(10/8)
BsoBI 1175 CCCGGG C^[CT]CG[GA]G
BspEI 29 TCCGGA T^CCGGA
BsrBI 58 CCGCTC CCGCTC(-3/-3)
BsrDI 321 CATTGC GCAATG(2/0)
BsrFI 183 GCCGGC [GA]^CCGG[CT]
BstAPI 12 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1082 GGATCC [GA]^GATC[CT]
Cfr10I 183 GCCGGC [GA]^CCGG[CT]
DraI 1018 TTTAAA TTT^AAA
DraIII 1650 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 6 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1524 ATGCAT ATGCA^T
FbaI 1869 TGATCA T^GATCA
FspI 329 TGCGCA TGC^GCA
HgaI 53 GCGTC GACGC(5/10)
HincII 1762 GTCAAC GT[CT]^[GA]AC
HindII 1762 GTCAAC GT[CT]^[GA]AC
MamI 441 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1082 GGATCC [GA]^GATC[CT]
MluNI 1793 TGGCCA TGG^CCA
MroI 29 TCCGGA T^CCGGA
MscI 1793 TGGCCA TGG^CCA
MspA1I 978 CAGCTG C[AC]G^C[GT]G
NaeI 183 GCCGGC GCC^GGC
NcoI 434 CCATGG C^CATGG
NgoMIV 183 GCCGGC G^CCGGC
NsiI 1524 ATGCAT ATGCA^T
PflMI 1382 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1359 CACGTG CAC^GTG
PmlI 1359 CACGTG CAC^GTG
PpuMI 769 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1765 AACGTT AA^CGTT
PvuII 978 CAGCTG CAG^CTG
SacI 1443 GAGCTC GAGCT^C
ScaI 239 AGTACT AGT^ACT
SexAI 931 ACCTGGT A^CC[AT]GGT
SmaI 1175 CCCGGG CCC^GGG
StuI 101 AGGCCT AGG^CCT
TaqI 649 TCGA T^CGA
TfiI 1204 GATTC G^A[AT]TC
Tsp509I 1015 AATT ^AATT
TthHB8I 649 TCGA T^CGA
Van91I 1382 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1082 GGATCC [GA]^GATC[CT]
XmaCI 1175 CCCGGG C^CCGGG
XmaI 1175 CCCGGG C^CCGGG
XmnI 1365 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 1330 GAGCAC G[AT]GC[AT]^C
1443 GAGCTC
BanII 1348 GAGCCC G[GA]GC[CT]^C
1443 GAGCTC
BsaAI 115 TACGTG [CT]AC^GT[GA]
1359 CACGTG
BsaWI 29 TCCGGA [AT]^CCGG[AT]
215 TCCGGT
BsgI 1615 CTGCAC GTGCAG(16/14)
1596 GTGCAG
BsiHKAI 1330 GAGCAC G[AT]GC[AT]^C
1443 GAGCTC
BspMI 308 ACCTGC ACCTGC(4/8)
1027 ACCTGC
CfoI 318 GCGC GCG^C
330 GCGC
DraII 14 AGGGCCT [GA]G^G[ACGT]CC[CT]
769 AGGTCCC
EaeI 344 TGGCCG [CT]^GGCC[GA]
1793 TGGCCA
EcoO109I 14 AGGGCCT [GA]G^G[ACGT]CC[CT]
769 AGGTCCC
EcoT14I 434 CCATGG C^C[AT][AT]GG
1257 CCTTGG
HhaI 318 GCGC GCG^C
330 GCGC
HinP1I 318 GCGC G^CGC
330 GCGC
MseI 1019 TTAA T^TAA
1622 TTAA
NspI 362 GCATGC [GA]CATG^[CT]
1522 GCATGC
SphI 362 GCATGC GCATG^C
1522 GCATGC
StyI 434 CCATGG C^C[AT][AT]GG
1257 CCTTGG
Tru9I 1019 TTAA T^TAA
1622 TTAA
XcmI 254 CCACGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
425 CCACCACCGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbsI BbvI BcnI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I TspRI