Restriction map for
pF1KE3911
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BfrI BglI BlnI BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1084 GTCTAC GT^[AC][GT]AC
AccIII 773 TCCGGA T^CCGGA
AlwNI 1672 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1533 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1679 TGGCCA TGG^CCA
BanI 1440 GGTGCC G^G[CT][GA]CC
BbvCI 2077 CCTCAGC CCTCAGC(-5/-2)
BfaI 1180 CTAG C^TAG
BlpI 1873 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1873 GCTCAGC GC^T[ACGT]AGC
BsaAI 1867 TACGTG [CT]AC^GT[GA]
BsaI 4 GAGACC GGTCTC(1/5)
BseAI 773 TCCGGA T^CCGGA
BsmI 1406 GCATTC GAATGC(1/-1)
BspEI 773 TCCGGA T^CCGGA
BssSI 883 CTCGTG CACGAG(-5/-1)
CelII 1873 GCTCAGC GC^T[ACGT]AGC
DsaI 1630 CCGCGG C^C[GA][CT]GG
EcoRV 139 GATATC GAT^ATC
FseI 428 GGCCGGCC GGCCGG^CC
KspI 1630 CCGCGG CCGC^GG
MaeI 1180 CTAG C^TAG
MluNI 1679 TGGCCA TGG^CCA
MroI 773 TCCGGA T^CCGGA
MscI 1679 TGGCCA TGG^CCA
MslI 1731 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI 619 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 1630 CCGCGG CCGC^GG
SapI 1536 GCTCTTC GCTCTTC(1/4)
SexAI 1610 ACCAGGT A^CC[AT]GGT
SmaI 399 CCCGGG CCC^GGG
SphI 1357 GCATGC GCATG^C
XcmI 830 CCATGTTCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 399 CCCGGG C^CCGGG
XmaI 399 CCCGGG C^CCGGG
XmnI 1533 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 1173 CGCG CG^CG
1631 CGCG
AcsI 183 AAATTC [GA]^AATT[CT]
1547 AAATTT
AfaI 1056 GTAC GT^AC
1608 GTAC
AflIII 1040 ACGTGT A^C[GA][CT]GT
1685 ACGTGT
Alw44I 1311 GTGCAC G^TGCAC
1858 GTGCAC
AocI 805 CCTGAGG CC^T[ACGT]AGG
1521 CCTGAGG
ApaLI 1311 GTGCAC G^TGCAC
1858 GTGCAC
ApoI 183 AAATTC [GA]^AATT[CT]
1547 AAATTT
BbsI 604 GAAGAC GAAGAC(2/6)
699 GAAGAC
BclI 54 TGATCA T^GATCA
1400 TGATCA
BglII 713 AGATCT A^GATCT
2009 AGATCT
BpuAI 604 GAAGAC GAAGAC(2/6)
699 GAAGAC
BspMI 878 ACCTGC ACCTGC(4/8)
1571 ACCTGC
BsrDI 160 CATTGC GCAATG(2/0)
349 GCAATG
BstUI 1173 CGCG CG^CG
1631 CGCG
BstXI 254 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
956 CCAACGTCCTGG
Bsu36I 805 CCTGAGG CC^T[ACGT]AGG
1521 CCTGAGG
Eco81I 805 CCTGAGG CC^T[ACGT]AGG
1521 CCTGAGG
EcoNI 531 CCTGTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
750 CCTCTGGCAGG
EcoT14I 83 CCAAGG C^C[AT][AT]GG
224 CCAAGG
FbaI 54 TGATCA T^GATCA
1400 TGATCA
MseI 176 TTAA T^TAA
815 TTAA
MvnI 1173 CGCG CG^CG
1631 CGCG
NspI 1357 GCATGC [GA]CATG^[CT]
1736 ACATGC
PstI 820 CTGCAG CTGCA^G
1486 CTGCAG
PvuII 255 CAGCTG CAG^CTG
1135 CAGCTG
RsaI 1056 GTAC GT^AC
1608 GTAC
SacI 1183 GAGCTC GAGCT^C
1900 GAGCTC
SfaNI 592 GATGC GCATC(5/9)
1324 GATGC
StyI 83 CCAAGG C^C[AT][AT]GG
224 CCAAGG
Tru9I 176 TTAA T^TAA
815 TTAA
Tsp45I 844 GTCAC ^GT[GC]AC
1046 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI Sau3AI
Sau96I ScrFI SfcI SmlI TaqI
TfiI TseI Tsp509I TspRI TthHB8I
Van91I XhoII