Restriction map for
pF1KE1429
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsiEI BsiWI BsmBI
BspDI BspHI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 208 GTCTAC GT^[AC][GT]AC
AcsI 1171 GAATTC [GA]^AATT[CT]
AcyI 1385 GGCGCC G[GA]^CG[CT]C
AflIII 1004 ACATGT A^C[GA][CT]GT
AocI 537 CCTGAGG CC^T[ACGT]AGG
ApaI 438 GGGCCC GGGCC^C
ApoI 1171 GAATTC [GA]^AATT[CT]
Asp700I 927 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 831 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 326 CCCGGG C^[CT]CG[GA]G
BanI 1385 GGCGCC G^G[CT][GA]CC
BbeI 1385 GGCGCC GGCGC^C
BfaI 1246 CTAG C^TAG
BsaHI 1385 GGCGCC G[GA]^CG[CT]C
BsgI 385 GTGCAG GTGCAG(16/14)
BsmI 1463 GCATTC GAATGC(1/-1)
BsoBI 326 CCCGGG C^[CT]CG[GA]G
Bsp1407I 854 TGTACA T^GTACA
BspLU11I 1004 ACATGT A^CATGT
BsrDI 706 CATTGC GCAATG(2/0)
BsrGI 854 TGTACA T^GTACA
BsrI 296 ACTGG ACTGG(1/-1)
BstXI 1241 CCACTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 537 CCTGAGG CC^T[ACGT]AGG
Eco81I 537 CCTGAGG CC^T[ACGT]AGG
EcoRI 1171 GAATTC G^AATTC
HaeII 1385 GGCGCC [GA]GCGC^[CT]
Hin1I 1385 GGCGCC G[GA]^CG[CT]C
HindIII 100 AAGCTT A^AGCTT
KasI 1385 GGCGCC G^GCGCC
MaeI 1246 CTAG C^TAG
MaeII 1313 ACGT A^CGT
MseI 98 TTAA T^TAA
MspA1I 1054 CAGCTG C[AC]G^C[GT]G
NarI 1385 GGCGCC GG^CGCC
NspI 1004 ACATGT [GA]CATG^[CT]
PflFI 831 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1124 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1054 CAGCTG CAG^CTG
SacI 754 GAGCTC GAGCT^C
SfoI 1385 GGCGCC GGC^GCC
SmaI 326 CCCGGG CCC^GGG
SmlI 472 CTCAAG C^T[CT][GA]AG
SspBI 854 TGTACA T^GTACA
TaqI 1274 TCGA T^CGA
Tru9I 98 TTAA T^TAA
Tsp509I 1172 AATT ^AATT
Tth111I 831 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1274 TCGA T^CGA
Van91I 1124 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 326 CCCGGG C^CCGGG
XmaI 326 CCCGGG C^CCGGG
XmnI 927 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 24 TCCGGA T^CCGGA
416 TCCGGA
AhdI 1351 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1531 GACGAGCTGTC
Alw44I 376 GTGCAC G^TGCAC
733 GTGCAC
AlwNI 256 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1333 CAGCCCCTG
ApaLI 376 GTGCAC G^TGCAC
733 GTGCAC
AspEI 1351 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1531 GACGAGCTGTC
BalI 761 TGGCCA TGG^CCA
995 TGGCCA
BbsI 1029 GAAGAC GAAGAC(2/6)
1038 GAAGAC
BcnI 326 CCCGG CC^[GC]GG
327 CCGGG
BpuAI 1029 GAAGAC GAAGAC(2/6)
1038 GAAGAC
BsaI 1415 GAGACC GGTCTC(1/5)
107 GGTCTC
BsaWI 24 TCCGGA [AT]^CCGG[AT]
416 TCCGGA
BseAI 24 TCCGGA T^CCGGA
416 TCCGGA
BspEI 24 TCCGGA T^CCGGA
416 TCCGGA
BstAPI 255 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
378 GCACGCTGTGC
DdeI 538 CTGAG C^T[ACGT]AG
1259 CTCAG
DraIII 379 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1223 CACTCTGTG
EaeI 761 TGGCCA [CT]^GGCC[GA]
995 TGGCCA
Eam1105I 1351 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1531 GACGAGCTGTC
EarI 1323 GAAGAG CTCTTC(1/4)
1552 GAAGAG
EcoNI 942 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1161 CCTCATGGAGG
FauI 14 CCCGC CCCGC(4/6)
291 GCGGG
Ksp632I 1323 GAAGAG CTCTTC(1/4)
1552 GAAGAG
MluNI 761 TGGCCA TGG^CCA
995 TGGCCA
MroI 24 TCCGGA T^CCGGA
416 TCCGGA
MscI 761 TGGCCA TGG^CCA
995 TGGCCA
NciI 326 CCCGG CC^[GC]GG
327 CCGGG
PstI 253 CTGCAG CTGCA^G
776 CTGCAG
SfcI 253 CTGCAG C^T[GA][CT]AG
776 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
TseI Tsp45I TspRI XcmI XhoII