Restriction map for
pF1KB0481
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 990 GACGTC GACGT^C
AccII 1076 CGCG CG^CG
AfaI 186 GTAC GT^AC
AhdI 985 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1381 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 342 GGGCCC GGGCC^C
AspEI 985 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 529 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 14 GTCTTC GAAGAC(2/6)
BbvCI 1467 CCTCAGC CCTCAGC(-5/-2)
BfaI 1408 CTAG C^TAG
BpuAI 14 GTCTTC GAAGAC(2/6)
BsaBI 1413 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 402 GAGACC GGTCTC(1/5)
BseRI 1126 CTCCTC GAGGAG(10/8)
BstAPI 1044 GCAGGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1076 CGCG CG^CG
BstYI 149 AGATCC [GA]^GATC[CT]
DsaI 894 CCGTGG C^C[GA][CT]GG
Eam1105I 985 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 459 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1187 GATATC GAT^ATC
EcoT14I 8 CCAAGG C^C[AT][AT]GG
HaeII 650 AGCGCC [GA]GCGC^[CT]
MaeI 1408 CTAG C^TAG
MamI 1413 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 149 AGATCC [GA]^GATC[CT]
MspA1I 1114 CAGCTG C[AC]G^C[GT]G
MvnI 1076 CGCG CG^CG
PflFI 529 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 779 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1114 CAGCTG CAG^CTG
RsaI 186 GTAC GT^AC
StyI 8 CCAAGG C^C[AT][AT]GG
Tth111I 529 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 779 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 149 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 571 TCCGGA T^CCGGA
1349 TCCGGA
AcsI 240 GAATTT [GA]^AATT[CT]
1299 AAATTT
AcyI 83 GGCGTC G[GA]^CG[CT]C
990 GACGTC
AlwI 150 GATCC GGATC(4/5)
1397 GGATC
ApoI 240 GAATTT [GA]^AATT[CT]
1299 AAATTT
BcnI 450 CCGGG CC^[GC]GG
1293 CCCGG
BlpI 1320 GCTGAGC GC^T[ACGT]AGC
1402 GCTGAGC
Bpu1102I 1320 GCTGAGC GC^T[ACGT]AGC
1402 GCTGAGC
BsaHI 83 GGCGTC G[GA]^CG[CT]C
990 GACGTC
BsaWI 571 TCCGGA [AT]^CCGG[AT]
1349 TCCGGA
BseAI 571 TCCGGA T^CCGGA
1349 TCCGGA
BsgI 544 CTGCAC GTGCAG(16/14)
1042 GTGCAG
BsmBI 85 CGTCTC CGTCTC(1/5)
202 GAGACG
BspEI 571 TCCGGA T^CCGGA
1349 TCCGGA
CelII 1320 GCTGAGC GC^T[ACGT]AGC
1402 GCTGAGC
DpnII 150 GATC ^GATC
1398 GATC
DraII 341 AGGGCCC [GA]G^G[ACGT]CC[CT]
342 GGGCCCC
EcoO109I 341 AGGGCCC [GA]G^G[ACGT]CC[CT]
342 GGGCCCC
HgaI 84 GCGTC GACGC(5/10)
731 GCGTC
Hin1I 83 GGCGTC G[GA]^CG[CT]C
990 GACGTC
MboI 150 GATC ^GATC
1398 GATC
MroI 571 TCCGGA T^CCGGA
1349 TCCGGA
MseI 332 TTAA T^TAA
1541 TTAA
NciI 450 CCGGG CC^[GC]GG
1293 CCCGG
NdeII 150 GATC ^GATC
1398 GATC
PstI 1003 CTGCAG CTGCA^G
1180 CTGCAG
Sau3AI 150 GATC ^GATC
1398 GATC
Tru9I 332 TTAA T^TAA
1541 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AvaI AvaII BalI
BanI BanII BbvI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MluNI MnlI MscI
MslI MspI MvaI MwoI NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
SfcI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI