Restriction map for
pF1KB9985
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsiWI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
KasI KpnI Ksp632I KspI MamI
MluI MroI MspA1I MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1189 GACGTC GACGT^C
AccI 634 GTCTAC GT^[AC][GT]AC
AcyI 1189 GACGTC G[GA]^CG[CT]C
AflIII 902 ACACGT A^C[GA][CT]GT
AlwNI 1052 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1659 CCCGGG C^[CT]CG[GA]G
BamHI 1677 GGATCC G^GATCC
BanII 270 GGGCTC G[GA]GC[CT]^C
BglII 771 AGATCT A^GATCT
BpmI 1075 CTGGAG CTGGAG(16/14)
BsaHI 1189 GACGTC G[GA]^CG[CT]C
BsaI 1463 GAGACC GGTCTC(1/5)
BsgI 232 GTGCAG GTGCAG(16/14)
BsiEI 738 CGATCG CG[GA][CT]^CG
BsmBI 1362 GAGACG CGTCTC(1/5)
BsmI 1256 GCATTC GAATGC(1/-1)
BsoBI 1659 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1553 TGTACA T^GTACA
BsrDI 89 GCAATG GCAATG(2/0)
BsrGI 1553 TGTACA T^GTACA
BstXI 531 CCACACACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 234 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 1496 GCGC GCG^C
DraI 1702 TTTAAA TTT^AAA
DrdI 405 GACAGCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 189 GCGGG CCCGC(4/6)
HaeII 1495 AGCGCC [GA]GCGC^[CT]
HhaI 1496 GCGC GCG^C
Hin1I 1189 GACGTC G[GA]^CG[CT]C
HinP1I 1496 GCGC G^CGC
HincII 1168 GTTAAC GT[CT]^[GA]AC
HindII 1168 GTTAAC GT[CT]^[GA]AC
HpaI 1168 GTTAAC GTT^AAC
MfeI 1145 CAATTG C^AATTG
MunI 1145 CAATTG C^AATTG
NcoI 1207 CCATGG C^CATGG
NdeI 656 CATATG CA^TATG
PflMI 552 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 253 GACTC GAGTC(4/5)
PshAI 1185 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 738 CGATCG CGAT^CG
SmaI 1659 CCCGGG CCC^GGG
SspBI 1553 TGTACA T^GTACA
StuI 1617 AGGCCT AGG^CCT
TaqI 741 TCGA T^CGA
TthHB8I 741 TCGA T^CGA
Van91I 552 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1659 CCCGGG C^CCGGG
XmaI 1659 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspI 962 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1231 GACTTTGTC
BalI 1122 TGGCCA TGG^CCA
1520 TGGCCA
BanI 439 GGCACC G^G[CT][GA]CC
1203 GGTGCC
BfaI 308 CTAG C^TAG
809 CTAG
BsaAI 688 TACGTG [CT]AC^GT[GA]
903 CACGTA
BsmFI 561 GGGAC GGGAC(10/14)
1384 GGGAC
BspHI 75 TCATGA T^CATGA
801 TCATGA
BsrFI 442 ACCGGC [GA]^CCGG[CT]
1200 GCCGGT
BssSI 1434 CACGAG CACGAG(-5/-1)
265 CTCGTG
BstYI 771 AGATCT [GA]^GATC[CT]
1677 GGATCC
Cfr10I 442 ACCGGC [GA]^CCGG[CT]
1200 GCCGGT
DraII 960 AGGACCT [GA]G^G[ACGT]CC[CT]
1053 AGGACCT
DsaI 1207 CCATGG C^C[GA][CT]GG
1262 CCACGG
EcoO109I 960 AGGACCT [GA]G^G[ACGT]CC[CT]
1053 AGGACCT
EcoT14I 1207 CCATGG C^C[AT][AT]GG
1248 CCAAGG
MaeI 308 CTAG C^TAG
809 CTAG
MflI 771 AGATCT [GA]^GATC[CT]
1677 GGATCC
MluNI 1122 TGGCCA TGG^CCA
1520 TGGCCA
MscI 1122 TGGCCA TGG^CCA
1520 TGGCCA
MwoI 1247 GCCAAGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1489 GCAAACAGCGC
PflFI 962 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1231 GACTTTGTC
PpuMI 960 AGGACCT [GA]G^G[AT]CC[CT]
1053 AGGACCT
RcaI 75 TCATGA T^CATGA
801 TCATGA
StyI 1207 CCATGG C^C[AT][AT]GG
1248 CCAAGG
Tth111I 962 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1231 GACTTTGTC
XhoII 771 AGATCT [GA]^GATC[CT]
1677 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI