Restriction map for
pF1KB8501
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspHI AviII
AvrII BalI BanI BanII BbeI
BbrPI BcgI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BseAI BseRI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspMI BsrFI BsrGI
BssHII BstBI BstEII BstPI BstXI
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
HgaI Hin1I HindIII KasI KpnI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tsp45I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 219 CGCG CG^CG
AflIII 2445 ACATGT A^C[GA][CT]GT
AhdI 1843 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 170 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 76 CCTCAGG CC^T[ACGT]AGG
AspEI 1843 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1225 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1601 CTCGAG C^[CT]CG[GA]G
BbvCI 3 GCTGAGG CCTCAGC(-5/-2)
BciVI 2409 GTATCC GTATCC(6/5)
BglII 14 AGATCT A^GATCT
BsaI 141 GGTCTC GGTCTC(1/5)
BsaWI 185 ACCGGA [AT]^CCGG[AT]
BsiEI 183 CGACCG CG[GA][CT]^CG
BsoBI 1601 CTCGAG C^[CT]CG[GA]G
BspLU11I 2445 ACATGT A^CATGT
BsrBI 69 CCGCTC CCGCTC(-3/-3)
BsrDI 1822 CATTGC GCAATG(2/0)
BssSI 326 CACGAG CACGAG(-5/-1)
Bst1107I 2484 GTATAC GTA^TAC
BstAPI 829 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 219 CGCG CG^CG
BstZ17I 2484 GTATAC GTA^TAC
Bsu36I 76 CCTCAGG CC^T[ACGT]AGG
CfoI 220 GCGC GCG^C
DraII 81 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 232 CGGCCA [CT]^GGCC[GA]
Eam1105I 1843 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2515 GAAGAG CTCTTC(1/4)
Eco81I 76 CCTCAGG CC^T[ACGT]AGG
EcoNI 795 CCTAATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 81 GGGTCCC [GA]G^G[ACGT]CC[CT]
HhaI 220 GCGC GCG^C
HinP1I 220 GCGC G^CGC
HincII 1093 GTTAAC GT[CT]^[GA]AC
HindII 1093 GTTAAC GT[CT]^[GA]AC
HpaI 1093 GTTAAC GTT^AAC
Ksp632I 2515 GAAGAG CTCTTC(1/4)
MaeII 166 ACGT A^CGT
MspA1I 830 CAGCTG C[AC]G^C[GT]G
MvnI 219 CGCG CG^CG
PaeR7I 1601 CTCGAG C^TCGAG
PflFI 1225 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 81 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 2540 CTGCAG CTGCA^G
PvuII 830 CAGCTG CAG^CTG
ScaI 2170 AGTACT AGT^ACT
SspI 2126 AATATT AAT^ATT
StuI 7 AGGCCT AGG^CCT
Tth111I 1225 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1601 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BamHI 1293 GGATCC G^GATCC
2712 GGATCC
BbsI 1360 GAAGAC GAAGAC(2/6)
1571 GTCTTC
BcnI 39 CCCGG CC^[GC]GG
230 CCCGG
BpmI 103 CTCCAG CTGGAG(16/14)
1034 CTGGAG
BpuAI 1360 GAAGAC GAAGAC(2/6)
1571 GTCTTC
BsmFI 2505 GGGAC GGGAC(10/14)
83 GTCCC
EcoRI 657 GAATTC G^AATTC
781 GAATTC
EcoRV 1545 GATATC GAT^ATC
2182 GATATC
EcoT22I 771 ATGCAT ATGCA^T
2428 ATGCAT
MfeI 2567 CAATTG C^AATTG
2707 CAATTG
MslI 1614 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2395 CATATTGATG
MunI 2567 CAATTG C^AATTG
2707 CAATTG
NciI 39 CCCGG CC^[GC]GG
230 CCCGG
NdeI 578 CATATG CA^TATG
2111 CATATG
NsiI 771 ATGCAT ATGCA^T
2428 ATGCAT
NspI 2426 ACATGC [GA]CATG^[CT]
2445 ACATGT
PacI 967 TTAATTAA TTAAT^TAA
1720 TTAATTAA
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AvaII BbvI BclI BfaI BsaJI
BsgI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI