################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MroI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 336 GTAC GT^AC AlwI 309 GATCC GGATC(4/5) AlwNI 355 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 80 AGCGCT AGC^GCT AspHI 61 GAGCTC G[AT]GC[AT]^C AviII 266 TGCGCA TGC^GCA BalI 209 TGGCCA TGG^CCA BanI 46 GGCACC G^G[CT][GA]CC BbvCI 420 CCTCAGC CCTCAGC(-5/-2) BglI 253 GCCTTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 300 GCTGAGC GC^T[ACGT]AGC BpmI 12 CTCCAG CTGGAG(16/14) Bpu1102I 300 GCTGAGC GC^T[ACGT]AGC BsaAI 403 TACGTG [CT]AC^GT[GA] BsiEI 87 CGGCCG CG[GA][CT]^CG BsiHKAI 61 GAGCTC G[AT]GC[AT]^C BsmFI 27 GTCCC GGGAC(10/14) BsrI 237 CCAGT ACTGG(1/-1) BssHII 170 GCGCGC G^CGCGC BssSI 412 CACGAG CACGAG(-5/-1) BstYI 308 AGATCC [GA]^GATC[CT] CelII 300 GCTGAGC GC^T[ACGT]AGC DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 32 CCGTGG C^C[GA][CT]GG EagI 87 CGGCCG C^GGCCG EclXI 87 CGGCCG C^GGCCG Eco47III 80 AGCGCT AGC^GCT Eco52I 87 CGGCCG C^GGCCG EcoNI 348 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT] FspI 266 TGCGCA TGC^GCA HgaI 315 GACGC GACGC(5/10) MboII 121 GAAGA GAAGA(8/7) MflI 308 AGATCC [GA]^GATC[CT] MluNI 209 TGGCCA TGG^CCA MscI 209 TGGCCA TGG^CCA MspA1I 393 CAGCTG C[AC]G^C[GT]G PaeR7I 64 CTCGAG C^TCGAG PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 393 CAGCTG CAG^CTG RsaI 336 GTAC GT^AC SacI 61 GAGCTC GAGCT^C SexAI 353 ACCAGGT A^CC[AT]GGT SfaNI 203 GCATC GCATC(5/9) SmaI 138 CCCGGG CCC^GGG StuI 251 AGGCCT AGG^CCT XhoI 64 CTCGAG C^TCGAG XhoII 308 AGATCC [GA]^GATC[CT] XmaCI 138 CCCGGG C^CCGGG XmaI 138 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaI 64 CTCGAG C^[CT]CG[GA]G 138 CCCGGG AvaII 357 GGTCC G^G[AT]CC 461 GGTCC BanII 9 GGGCTC G[GA]GC[CT]^C 61 GAGCTC BcnI 138 CCCGG CC^[GC]GG 139 CCGGG BsoBI 64 CTCGAG C^[CT]CG[GA]G 138 CCCGGG BstF5I 204 CATCC GGATG(2/0) 276 CATCC DdeI 301 CTGAG C^T[ACGT]AG 421 CTCAG DpnII 273 GATC ^GATC 309 GATC FokI 204 CATCC GGATG(9/13) 276 CATCC HapII 90 CCGG C^CGG 139 CCGG HpaII 90 CCGG C^CGG 139 CCGG MaeII 215 ACGT A^CGT 404 ACGT MboI 273 GATC ^GATC 309 GATC MspI 90 CCGG C^CGG 139 CCGG NciI 138 CCCGG CC^[GC]GG 139 CCGGG NdeII 273 GATC ^GATC 309 GATC NlaIII 447 CATG CATG^ 465 CATG NlaIV 10 GGCTCC GG[ACGT]^[ACGT]CC 46 GGCACC Sau3AI 273 GATC ^GATC 309 GATC SmlI 64 CTCGAG C^T[CT][GA]AG 319 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AccII AciI AluI BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I EaeI EcoRII Fnu4HI HaeII HaeIII HhaI HinP1I ItaI MnlI MvaI MvnI MwoI PstI Sau96I ScrFI SfcI TaqI TseI TthHB8I