Restriction map for
pF1KB7974
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MflI MluI MseI MunI MvnI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 21 GGCGCC G[GA]^CG[CT]C
AlwI 1069 GATCC GGATC(4/5)
AlwNI 791 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 464 TGGCCA TGG^CCA
BbeI 21 GGCGCC GGCGC^C
BbsI 900 GTCTTC GAAGAC(2/6)
BbvCI 733 CCTCAGC CCTCAGC(-5/-2)
BglI 466 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 900 GTCTTC GAAGAC(2/6)
BsaHI 21 GGCGCC G[GA]^CG[CT]C
BspHI 423 TCATGA T^CATGA
BsrFI 47 GCCGGC [GA]^CCGG[CT]
BssSI 1076 CACGAG CACGAG(-5/-1)
BstXI 185 CCAATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 22 GCGC GCG^C
Cfr10I 47 GCCGGC [GA]^CCGG[CT]
DraIII 349 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 549 GAAGAG CTCTTC(1/4)
EcoNI 93 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 107 CCAAGG C^C[AT][AT]GG
FauI 327 GCGGG CCCGC(4/6)
HaeII 21 GGCGCC [GA]GCGC^[CT]
HhaI 22 GCGC GCG^C
Hin1I 21 GGCGCC G[GA]^CG[CT]C
HinP1I 22 GCGC G^CGC
HinfI 540 GATTC G^A[ACGT]TC
KasI 21 GGCGCC G^GCGCC
Ksp632I 549 GAAGAG CTCTTC(1/4)
MluNI 464 TGGCCA TGG^CCA
MscI 464 TGGCCA TGG^CCA
MslI 1076 CACGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 47 GCCGGC GCC^GGC
NarI 21 GGCGCC GG^CGCC
NgoMIV 47 GCCGGC G^CCGGC
PflMI 774 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 423 TCATGA T^CATGA
SfiI 465 GGCCATCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 21 GGCGCC GGC^GCC
StyI 107 CCAAGG C^C[AT][AT]GG
TfiI 540 GATTC G^A[AT]TC
Van91I 774 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccIII 374 TCCGGA T^CCGGA
979 TCCGGA
AfaI 154 GTAC GT^AC
534 GTAC
ApaI 473 GGGCCC GGGCC^C
867 GGGCCC
AvaII 562 GGTCC G^G[AT]CC
982 GGACC
BsaI 1163 GAGACC GGTCTC(1/5)
514 GGTCTC
BsaWI 374 TCCGGA [AT]^CCGG[AT]
979 TCCGGA
BseAI 374 TCCGGA T^CCGGA
979 TCCGGA
BseRI 313 GAGGAG GAGGAG(10/8)
1105 GAGGAG
BsmAI 1163 GAGAC GTCTC(1/5)
515 GTCTC
BspEI 374 TCCGGA T^CCGGA
979 TCCGGA
DraII 252 GGGGCCT [GA]G^G[ACGT]CC[CT]
473 GGGCCCC
EaeI 464 TGGCCA [CT]^GGCC[GA]
889 CGGCCA
EcoO109I 252 GGGGCCT [GA]G^G[ACGT]CC[CT]
473 GGGCCCC
MroI 374 TCCGGA T^CCGGA
979 TCCGGA
RsaI 154 GTAC GT^AC
534 GTAC
SmlI 127 CTCAAG C^T[CT][GA]AG
298 CTCAAG
TaqI 212 TCGA T^CGA
311 TCGA
TthHB8I 212 TCGA T^CGA
311 TCGA
XcmI 774 CCAGGGAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1025 CCACAGGTGTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AspHI AvaI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI TseI Tsp45I
TspRI XmaCI XmaI