################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRII FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvaI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 510 CGCG CG^CG AfaI 77 GTAC GT^AC AflIII 916 ACATGT A^C[GA][CT]GT Alw44I 751 GTGCAC G^TGCAC AlwNI 220 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1445 CCTCAGG CC^T[ACGT]AGG ApaLI 751 GTGCAC G^TGCAC AvaI 703 CTCGAG C^[CT]CG[GA]G BanII 1453 GAGCTC G[GA]GC[CT]^C BbvI 292 GCTGC GCAGC(8/12) BciVI 1740 GGATAC GTATCC(6/5) BclI 189 TGATCA T^GATCA BcnI 866 CCGGG CC^[GC]GG BsmI 615 GAATGC GAATGC(1/-1) BsoBI 703 CTCGAG C^[CT]CG[GA]G BspLU11I 916 ACATGT A^CATGT BspMI 58 GCAGGT ACCTGC(4/8) BssKI 866 CCGGG ^CC[ACGT]GG BstF5I 1659 GGATG GGATG(2/0) BstUI 510 CGCG CG^CG BstXI 996 CCACTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1445 CCTCAGG CC^T[ACGT]AGG CfoI 509 GCGC GCG^C Eco81I 1445 CCTCAGG CC^T[ACGT]AGG EcoNI 499 CCTAAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 94 GAATTC G^AATTC EcoRV 384 GATATC GAT^ATC EcoT14I 893 CCAAGG C^C[AT][AT]GG FauI 51 GCGGG CCCGC(4/6) FbaI 189 TGATCA T^GATCA Fnu4HI 292 GCTGC GC^[ACGT]GC FokI 1659 GGATG GGATG(9/13) HapII 866 CCGG C^CGG HgaI 836 GCGTC GACGC(5/10) HhaI 509 GCGC GCG^C HinP1I 509 GCGC G^CGC HpaII 866 CCGG C^CGG ItaI 292 GCTGC GC^[ACGT]GC MspI 866 CCGG C^CGG MvnI 510 CGCG CG^CG NciI 866 CCGGG CC^[GC]GG NlaIV 1472 GGTCCC GG[ACGT]^[ACGT]CC PaeR7I 703 CTCGAG C^TCGAG PvuII 1030 CAGCTG CAG^CTG RsaI 77 GTAC GT^AC SacI 1453 GAGCTC GAGCT^C ScrFI 866 CCGGG CC^[ACGT]GG SphI 1133 GCATGC GCATG^C StyI 893 CCAAGG C^C[AT][AT]GG TseI 292 GCTGC G^C[AT]GC XbaI 1584 TCTAGA T^CTAGA XhoI 703 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 51 GCGG CCGC(-3/-1) 856 GCGG AlwI 879 GGATC GGATC(4/5) 1602 GGATC Asp700I 161 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 549 GAAGGCTTTC AvaII 1472 GGTCC G^G[AT]CC 1713 GGACC BbsI 428 GTCTTC GAAGAC(2/6) 1120 GTCTTC BpmI 1590 CTCCAG CTGGAG(16/14) 1313 CTGGAG BpuAI 428 GTCTTC GAAGAC(2/6) 1120 GTCTTC BsaJI 866 CCGGGG C^C[ACGT][ACGT]GG 893 CCAAGG BsgI 1571 GTGCAG GTGCAG(16/14) 1595 GTGCAG BsiYI 499 CCTAAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 565 CCGAAAACAGG BslI 499 CCTAAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 565 CCGAAAACAGG BsmFI 1705 GGGAC GGGAC(10/14) 1473 GTCCC Cfr13I 1472 GGTCC G^G[ACGT]CC 1713 GGACC EcoT22I 617 ATGCAT ATGCA^T 1135 ATGCAT HaeIII 126 GGCC GG^CC 897 GGCC HincII 708 GTTGAC GT[CT]^[GA]AC 1776 GTCAAC HindII 708 GTTGAC GT[CT]^[GA]AC 1776 GTCAAC MaeII 777 ACGT A^CGT 989 ACGT MspA1I 49 CAGCGG C[AC]G^C[GT]G 1030 CAGCTG NdeI 1241 CATATG CA^TATG 1413 CATATG NsiI 617 ATGCAT ATGCA^T 1135 ATGCAT SapI 871 GAAGAGC GCTCTTC(1/4) 1693 GCTCTTC Sau96I 1472 GGTCC G^G[ACGT]CC 1713 GGACC XmnI 161 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 549 GAAGGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AcsI AluI ApoI AspHI BfaI BmyI BsiHKAI BsmAI Bsp1286I BsrI Cac8I DdeI DpnII DraI EarI HinfI HphI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NspI PleI Sau3AI SfaNI SfcI SmlI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I