Restriction map for
pF1KE1083
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MluI
MluNI MroI MscI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI StyI SwaI
TfiI Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 747 CCGC CCGC(-3/-1)
AcsI 399 GAATTC [GA]^AATT[CT]
AfaI 818 GTAC GT^AC
ApoI 399 GAATTC [GA]^AATT[CT]
AseI 423 ATTAAT AT^TAAT
AsnI 423 ATTAAT AT^TAAT
Asp700I 835 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1059 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 692 GGGCTC G[GA]GC[CT]^C
BbsI 1036 GAAGAC GAAGAC(2/6)
BglII 741 AGATCT A^GATCT
BpuAI 1036 GAAGAC GAAGAC(2/6)
BsaBI 433 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 89 CCGTGG C^C[ACGT][ACGT]GG
BsmAI 849 GAGAC GTCTC(1/5)
BsmBI 849 GAGACG CGTCTC(1/5)
BsmFI 944 GGGAC GGGAC(10/14)
BspDI 310 ATCGAT AT^CGAT
BssKI 624 CCTGG ^CC[ACGT]GG
BssSI 16 CACGAG CACGAG(-5/-1)
BstNI 624 CCTGG CC^[AT]GG
BstYI 741 AGATCT [GA]^GATC[CT]
Cac8I 981 GCCAGC GC[ACGT]^[ACGT]GC
ClaI 310 ATCGAT AT^CGAT
DraII 944 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 35 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 89 CCGTGG C^C[GA][CT]GG
EcoO109I 944 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 399 GAATTC G^AATTC
EcoRII 624 CCTGG ^CC[AT]GG
EcoT22I 556 ATGCAT ATGCA^T
HaeIII 980 GGCC GG^CC
MaeII 178 ACGT A^CGT
MamI 433 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 104 CAATTG C^AATTG
MflI 741 AGATCT [GA]^GATC[CT]
MunI 104 CAATTG C^AATTG
MvaI 624 CCTGG CC^[AT]GG
NsiI 556 ATGCAT ATGCA^T
NspI 1040 ACATGC [GA]CATG^[CT]
PflFI 1059 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 944 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 423 ATTAAT AT^TAAT
PstI 532 CTGCAG CTGCA^G
RsaI 818 GTAC GT^AC
SapI 835 GAAGAGC GCTCTTC(1/4)
ScrFI 624 CCTGG CC^[ACGT]GG
SfcI 532 CTGCAG C^T[GA][CT]AG
SmlI 794 CTCAAG C^T[CT][GA]AG
SpeI 160 ACTAGT A^CTAGT
SspI 77 AATATT AAT^ATT
Tsp45I 1057 GTGAC ^GT[GC]AC
Tth111I 1059 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 741 AGATCT [GA]^GATC[CT]
XmnI 835 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 313 GATCC GGATC(4/5)
308 GGATC
AspHI 41 GTGCTC G[AT]GC[AT]^C
998 GAGCAC
BfaI 161 CTAG C^TAG
1015 CTAG
BsiHKAI 41 GTGCTC G[AT]GC[AT]^C
998 GAGCAC
BsrDI 49 CATTGC GCAATG(2/0)
242 GCAATG
BsrI 356 ACTGG ACTGG(1/-1)
989 CCAGT
EarI 835 GAAGAG CTCTTC(1/4)
846 GAAGAG
HinfI 4 GAGTC G^A[ACGT]TC
608 GAGTC
HphI 499 GGTGA GGTGA(8/7)
1018 GGTGA
Ksp632I 835 GAAGAG CTCTTC(1/4)
846 GAAGAG
MaeI 161 CTAG C^TAG
1015 CTAG
MaeIII 157 GTTAC ^GT[ACGT]AC
1057 GTGAC
MspA1I 115 CAGCTG C[AC]G^C[GT]G
1052 CAGCTG
PleI 4 GAGTC GAGTC(4/5)
608 GAGTC
PvuII 115 CAGCTG CAG^CTG
1052 CAGCTG
TaqI 311 TCGA T^CGA
561 TCGA
TthHB8I 311 TCGA T^CGA
561 TCGA
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AvaII BbvI BmyI BseRI
Bsp1286I BstF5I Cfr13I DdeI DpnII
Fnu4HI FokI ItaI MboI MboII
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I SfaNI
Tru9I TseI Tsp509I TspRI