Restriction map for
pF1KB5853
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwNI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AvaI AviII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tru9I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 568 GCGG CCGC(-3/-1)
AhdI 169 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 246 CCTGAGG CC^T[ACGT]AGG
Aor51HI 266 AGCGCT AGC^GCT
AspEI 169 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 13 GTGCTC G[AT]GC[AT]^C
AspI 789 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 741 CCTAGG C^CTAGG
BanII 340 GAGCCC G[GA]GC[CT]^C
BbvCI 198 CCTCAGC CCTCAGC(-5/-2)
BlnI 741 CCTAGG C^CTAGG
BpmI 541 CTCCAG CTGGAG(16/14)
BsaI 915 GGTCTC GGTCTC(1/5)
BsiHKAI 13 GTGCTC G[AT]GC[AT]^C
BsmAI 916 GTCTC GTCTC(1/5)
BsmI 124 GCATTC GAATGC(1/-1)
BsrDI 674 CATTGC GCAATG(2/0)
BstAPI 774 GCACATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 227 CCATCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 905 GGATCT [GA]^GATC[CT]
Bsu36I 246 CCTGAGG CC^T[ACGT]AGG
Eam1105I 169 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 814 CTCTTC CTCTTC(1/4)
Eco47III 266 AGCGCT AGC^GCT
Eco81I 246 CCTGAGG CC^T[ACGT]AGG
EcoNI 198 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 741 CCTAGG C^C[AT][AT]GG
HaeII 266 AGCGCT [GA]GCGC^[CT]
Ksp632I 814 CTCTTC CTCTTC(1/4)
MflI 905 GGATCT [GA]^GATC[CT]
MspA1I 544 CAGCTG C[AC]G^C[GT]G
NdeI 522 CATATG CA^TATG
PflFI 789 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 238 GACTC GAGTC(4/5)
PvuII 544 CAGCTG CAG^CTG
SexAI 407 ACCTGGT A^CC[AT]GGT
SfcI 720 CTACAG C^T[GA][CT]AG
SspI 100 AATATT AAT^ATT
StyI 741 CCTAGG C^C[AT][AT]GG
Tth111I 789 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 629 CCATGACTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 905 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 795 GTCTAC GT^[AC][GT]AC
874 GTAGAC
AfaI 363 GTAC GT^AC
950 GTAC
AlwI 21 GGATC GGATC(4/5)
905 GGATC
BalI 489 TGGCCA TGG^CCA
548 TGGCCA
BbvI 288 GCAGC GCAGC(8/12)
143 GCTGC
BcnI 191 CCGGG CC^[GC]GG
680 CCCGG
BmyI 13 GTGCTC G[AGT]GC[ACT]^C
340 GAGCCC
Bsp1286I 13 GTGCTC G[AGT]GC[ACT]^C
340 GAGCCC
Cac8I 546 GCTGGC GC[ACGT]^[ACGT]GC
750 GCTGGC
CfoI 267 GCGC GCG^C
606 GCGC
EaeI 489 TGGCCA [CT]^GGCC[GA]
548 TGGCCA
HapII 191 CCGG C^CGG
681 CCGG
HhaI 267 GCGC GCG^C
606 GCGC
HinP1I 267 GCGC G^CGC
606 GCGC
HpaII 191 CCGG C^CGG
681 CCGG
MboII 516 GAAGA GAAGA(8/7)
815 TCTTC
MluNI 489 TGGCCA TGG^CCA
548 TGGCCA
MscI 489 TGGCCA TGG^CCA
548 TGGCCA
MslI 254 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
387 CACCATCGTG
MspI 191 CCGG C^CGG
681 CCGG
NciI 191 CCGGG CC^[GC]GG
680 CCCGG
RsaI 363 GTAC GT^AC
950 GTAC
SfaNI 735 GATGC GCATC(5/9)
74 GCATC
TseI 143 GCTGC G^C[AT]GC
288 GCAGC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AvaII BfaI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MnlI MvaI MwoI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI TfiI Tsp45I Tsp509I TspRI