##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
Hin1I       HpaI        HpaII       KasI        KpnI        
KspI        MaeIII      MfeI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        Sau96I      SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp45I      TspRI       Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AfaI          558  GTAC                 GT^AC
AflIII         78  ACATGT               A^C[GA][CT]GT
Asp700I       736  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI          529  GAAGAC               GAAGAC(2/6)
BbvCI        1150  GCTGAGG              CCTCAGC(-5/-2)
BglII         665  AGATCT               A^GATCT
BpuAI         529  GAAGAC               GAAGAC(2/6)
BsaBI        1417  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          127  GTGCAG               GTGCAG(16/14)
BspHI         641  TCATGA               T^CATGA
BspLU11I       78  ACATGT               A^CATGT
BsrDI         718  GCAATG               GCAATG(2/0)
BsrI          276  ACTGG                ACTGG(1/-1)
BssKI         972  CCAGG                ^CC[ACGT]GG
BstNI         972  CCAGG                CC^[AT]GG
BstYI         665  AGATCT               [GA]^GATC[CT]
CfoI          156  GCGC                 GCG^C
DraI         1402  TTTAAA               TTT^AAA
EcoRII        972  CCAGG                ^CC[AT]GG
HgaI         1242  GCGTC                GACGC(5/10)
HhaI          156  GCGC                 GCG^C
HinP1I        156  GCGC                 G^CGC
HindIII       611  AAGCTT               A^AGCTT
HinfI         117  GAATC                G^A[ACGT]TC
HphI         1432  GGTGA                GGTGA(8/7)
MamI         1417  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          665  AGATCT               [GA]^GATC[CT]
MvaI          972  CCAGG                CC^[AT]GG
NheI         1137  GCTAGC               G^CTAGC
NlaIV           4  GGTTCC               GG[ACGT]^[ACGT]CC
PstI          524  CTGCAG               CTGCA^G
RcaI          641  TCATGA               T^CATGA
RsaI          558  GTAC                 GT^AC
SapI         1237  GAAGAGC              GCTCTTC(1/4)
ScaI          557  AGTACT               AGT^ACT
ScrFI         972  CCAGG                CC^[ACGT]GG
SphI         1362  GCATGC               GCATG^C
TaqI         1161  TCGA                 T^CGA
TfiI          117  GAATC                G^A[AT]TC
TthHB8I      1161  TCGA                 T^CGA
XcmI          476  CCAAAACCATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         665  AGATCT               [GA]^GATC[CT]
XmnI          736  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI          930  GCGG                 CCGC(-3/-1)
             1305  GCGG                
AseI         1170  ATTAAT               AT^TAAT
             1318  ATTAAT              
AsnI         1170  ATTAAT               AT^TAAT
             1318  ATTAAT              
BpmI          253  CTCCAG               CTGGAG(16/14)
              762  CTGGAG              
HaeIII        213  GGCC                 GG^CC
             1074  GGCC                
HincII        100  GTCAAC               GT[CT]^[GA]AC
              903  GTTGAC              
HindII        100  GTCAAC               GT[CT]^[GA]AC
              903  GTTGAC              
MspA1I        622  CAGCTG               C[AC]G^C[GT]G
             1201  CAGCTG              
PshBI        1170  ATTAAT               AT^TAAT
             1318  ATTAAT              
PvuII         622  CAGCTG               CAG^CTG
             1201  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BfaI        
BseRI       BsmI        BstF5I      Cac8I       DdeI        
DpnII       EarI        Fnu4HI      FokI        ItaI        
Ksp632I     MaeI        MaeII       MboI        MboII       
MnlI        MseI        MwoI        NdeII       NlaIII      
NspI        Sau3AI      SfaNI       SfcI        Tru9I       
TseI        Tsp509I