################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI Asp700I AspEI AspI AvaI AvaII AviII AvrII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I FauI FbaI Fnu4HI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI ItaI KasI KspI MaeII MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI TseI TspRI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 34 GGTACC G^GTACC AciI 148 CCGC CCGC(-3/-1) AcsI 657 AAATTT [GA]^AATT[CT] AflIII 208 ACATGT A^C[GA][CT]GT AgeI 31 ACCGGT A^CCGGT ApaI 228 GGGCCC GGGCC^C ApoI 657 AAATTT [GA]^AATT[CT] AseI 508 ATTAAT AT^TAAT AsnI 508 ATTAAT AT^TAAT Asp718I 34 GGTACC G^GTACC AspHI 355 GAGCTC G[AT]GC[AT]^C BalI 171 TGGCCA TGG^CCA BamHI 238 GGATCC G^GATCC BbsI 303 GTCTTC GAAGAC(2/6) BcnI 225 CCGGG CC^[GC]GG BfaI 769 CTAG C^TAG BpuAI 303 GTCTTC GAAGAC(2/6) BsaWI 31 ACCGGT [AT]^CCGG[AT] BseRI 716 CTCCTC GAGGAG(10/8) BsiHKAI 355 GAGCTC G[AT]GC[AT]^C BspHI 729 TCATGA T^CATGA BspLU11I 208 ACATGT A^CATGT BsrDI 786 CATTGC GCAATG(2/0) BsrFI 31 ACCGGT [GA]^CCGG[CT] BstEII 601 GGTCACC G^GT[ACGT]ACC BstPI 601 GGTCACC G^GT[ACGT]ACC BstYI 238 GGATCC [GA]^GATC[CT] Cfr10I 31 ACCGGT [GA]^CCGG[CT] DdeI 576 CTTAG C^T[ACGT]AG DrdI 763 GACTGTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 171 TGGCCA [CT]^GGCC[GA] EarI 352 GAAGAG CTCTTC(1/4) EcoO65I 601 GGTCACC G^GT[ACGT]ACC EcoT22I 445 ATGCAT ATGCA^T HgaI 301 GCGTC GACGC(5/10) HincII 526 GTTGAC GT[CT]^[GA]AC HindII 526 GTTGAC GT[CT]^[GA]AC KpnI 34 GGTACC GGTAC^C Ksp632I 352 GAAGAG CTCTTC(1/4) MaeI 769 CTAG C^TAG MflI 238 GGATCC [GA]^GATC[CT] MluNI 171 TGGCCA TGG^CCA MscI 171 TGGCCA TGG^CCA MseI 509 TTAA T^TAA MspA1I 628 CAGCTG C[AC]G^C[GT]G NciI 225 CCGGG CC^[GC]GG NsiI 445 ATGCAT ATGCA^T NspI 208 ACATGT [GA]CATG^[CT] PinAI 31 ACCGGT A^CCGGT PshBI 508 ATTAAT AT^TAAT PvuII 628 CAGCTG CAG^CTG RcaI 729 TCATGA T^CATGA SacI 355 GAGCTC GAGCT^C SapI 352 GAAGAGC GCTCTTC(1/4) ScaI 279 AGTACT AGT^ACT SmlI 673 CTTGAG C^T[CT][GA]AG StuI 370 AGGCCT AGG^CCT Tru9I 509 TTAA T^TAA Tsp45I 602 GTCAC ^GT[GC]AC XhoII 238 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 239 GATCC GGATC(4/5) 238 GGATC BanI 34 GGTACC G^G[CT][GA]CC 63 GGTGCC BanII 228 GGGCCC G[GA]GC[CT]^C 355 GAGCTC BsaJI 225 CCGGGG C^C[ACGT][ACGT]GG 390 CCCTGG BsiYI 385 CCGTGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 401 CCTGTAAATGG BslI 385 CCGTGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 401 CCTGTAAATGG BstNI 391 CCTGG CC^[AT]GG 517 CCTGG Cfr13I 228 GGGCC G^G[ACGT]CC 229 GGCCC DpnII 239 GATC ^GATC 722 GATC DraII 227 GGGGCCC [GA]G^G[ACGT]CC[CT] 228 GGGCCCT EcoO109I 227 GGGGCCC [GA]G^G[ACGT]CC[CT] 228 GGGCCCT EcoRII 391 CCTGG ^CC[AT]GG 517 CCTGG HapII 32 CCGG C^CGG 225 CCGG HinfI 108 GAGTC G^A[ACGT]TC 714 GACTC HpaII 32 CCGG C^CGG 225 CCGG HphI 534 GGTGA GGTGA(8/7) 603 TCACC MboI 239 GATC ^GATC 722 GATC MspI 32 CCGG C^CGG 225 CCGG MvaI 391 CCTGG CC^[AT]GG 517 CCTGG MwoI 188 GCAACTGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 812 GCAATTTAGGC NdeII 239 GATC ^GATC 722 GATC PleI 714 GACTC GAGTC(4/5) 108 GAGTC Sau3AI 239 GATC ^GATC 722 GATC Sau96I 228 GGGCC G^G[ACGT]CC 229 GGCCC TaqI 40 TCGA T^CGA 720 TCGA TthHB8I 40 TCGA T^CGA 720 TCGA XcmI 92 CCAACAATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 242 CCATTCGCAACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AfaI AluI BmyI Bsp1286I BsrI BssKI BstF5I Cac8I FokI HaeIII MaeIII MboII MnlI NlaIII NlaIV RsaI ScrFI SfaNI SfcI Tsp509I