Restriction map for
pF1KE6234
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccI AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BbvCI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KspI MamI
MfeI MluI MluNI MscI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 203 GGTACC G^GTACC
AccII 477 CGCG CG^CG
AccIII 341 TCCGGA T^CCGGA
Asp718I 203 GGTACC G^GTACC
AspHI 490 GTGCTC G[AT]GC[AT]^C
BamHI 338 GGATCC G^GATCC
BcgI 4 GCAAGCTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 671 TGATCA T^GATCA
BcnI 123 CCGGG CC^[GC]GG
BsaAI 728 TACGTA [CT]AC^GT[GA]
BseAI 341 TCCGGA T^CCGGA
BsiHKAI 490 GTGCTC G[AT]GC[AT]^C
BspEI 341 TCCGGA T^CCGGA
BspMI 376 ACCTGC ACCTGC(4/8)
BsrDI 415 GCAATG GCAATG(2/0)
BsrFI 645 GCCGGT [GA]^CCGG[CT]
BstF5I 138 GGATG GGATG(2/0)
BstUI 477 CGCG CG^CG
Cfr10I 645 GCCGGT [GA]^CCGG[CT]
EcoNI 215 CCTTCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 659 CCTTGG C^C[AT][AT]GG
FauI 806 GCGGG CCCGC(4/6)
FbaI 671 TGATCA T^GATCA
FokI 138 GGATG GGATG(9/13)
HindIII 6 AAGCTT A^AGCTT
KpnI 203 GGTACC GGTAC^C
MaeII 729 ACGT A^CGT
MaeIII 328 GTGAC ^GT[ACGT]AC
MroI 341 TCCGGA T^CCGGA
MslI 189 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 30 CAGCTG C[AC]G^C[GT]G
MvnI 477 CGCG CG^CG
NciI 123 CCGGG CC^[GC]GG
PvuII 30 CAGCTG CAG^CTG
SapI 721 GAAGAGC GCTCTTC(1/4)
SfaNI 307 GATGC GCATC(5/9)
SnaBI 728 TACGTA TAC^GTA
StyI 659 CCTTGG C^C[AT][AT]GG
TfiI 573 GAATC G^A[AT]TC
Tsp45I 328 GTGAC ^GT[GC]AC
TspRI 507 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 204 GTAC GT^AC
537 GTAC
BanII 125 GGGCTC G[GA]GC[CT]^C
453 GAGCCC
BfaI 221 CTAG C^TAG
703 CTAG
BpmI 458 CTGGAG CTGGAG(16/14)
847 CTGGAG
BsaI 15 GAGACC GGTCTC(1/5)
263 GAGACC
BsaWI 341 TCCGGA [AT]^CCGG[AT]
581 ACCGGA
BsrI 446 ACTGG ACTGG(1/-1)
686 ACTGG
BstYI 338 GGATCC [GA]^GATC[CT]
435 AGATCC
HaeIII 286 GGCC GG^CC
514 GGCC
MaeI 221 CTAG C^TAG
703 CTAG
MflI 338 GGATCC [GA]^GATC[CT]
435 AGATCC
NlaIII 155 CATG CATG^
189 CATG
PleI 330 GACTC GAGTC(4/5)
870 GACTC
PstI 499 CTGCAG CTGCA^G
532 CTGCAG
RsaI 204 GTAC GT^AC
537 GTAC
XhoII 338 GGATCC [GA]^GATC[CT]
435 AGATCC
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbsI BbvI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI HapII
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI SfcI TaqI TseI Tsp509I
TthHB8I