################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmBI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI FbaI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HpaI KasI KpnI MluI MluNI MscI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI Tsp45I Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AatII 97 GACGTC GACGT^C AccII 24 CGCG CG^CG AccIII 1218 TCCGGA T^CCGGA AcyI 97 GACGTC G[GA]^CG[CT]C AocI 33 CCTGAGG CC^T[ACGT]AGG AseI 1525 ATTAAT AT^TAAT AsnI 1525 ATTAAT AT^TAAT Asp700I 805 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1271 GAGCTC G[AT]GC[AT]^C AvaII 328 GGACC G^G[AT]CC BciVI 1544 GGATAC GTATCC(6/5) BglII 964 AGATCT A^GATCT BpmI 164 CTGGAG CTGGAG(16/14) BsaBI 1076 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 97 GACGTC G[GA]^CG[CT]C BsaI 841 GGTCTC GGTCTC(1/5) BsaWI 1218 TCCGGA [AT]^CCGG[AT] BseAI 1218 TCCGGA T^CCGGA BseRI 1037 CTCCTC GAGGAG(10/8) BsiHKAI 1271 GAGCTC G[AT]GC[AT]^C BsmI 978 GAATGC GAATGC(1/-1) Bsp1407I 117 TGTACA T^GTACA BspEI 1218 TCCGGA T^CCGGA BsrDI 504 CATTGC GCAATG(2/0) BsrGI 117 TGTACA T^GTACA BssSI 1061 CACGAG CACGAG(-5/-1) BstEII 2397 GGTTACC G^GT[ACGT]ACC BstPI 2397 GGTTACC G^GT[ACGT]ACC BstUI 24 CGCG CG^CG BstXI 318 CCATGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 964 AGATCT [GA]^GATC[CT] Bsu36I 33 CCTGAGG CC^T[ACGT]AGG DraII 5 AGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 2449 GACAGGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 679 CTCTTC CTCTTC(1/4) Eco81I 33 CCTGAGG CC^T[ACGT]AGG EcoO109I 5 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 2397 GGTTACC G^GT[ACGT]ACC EcoT22I 409 ATGCAT ATGCA^T HapII 1219 CCGG C^CGG Hin1I 97 GACGTC G[GA]^CG[CT]C HindIII 2379 AAGCTT A^AGCTT HpaII 1219 CCGG C^CGG Ksp632I 679 CTCTTC CTCTTC(1/4) KspI 23 CCGCGG CCGC^GG MamI 1076 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 623 CAATTG C^AATTG MflI 964 AGATCT [GA]^GATC[CT] MroI 1218 TCCGGA T^CCGGA MspI 1219 CCGG C^CGG MunI 623 CAATTG C^AATTG MvnI 24 CGCG CG^CG NcoI 450 CCATGG C^CATGG NsiI 409 ATGCAT ATGCA^T PflMI 1303 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 816 GACTC GAGTC(4/5) PshBI 1525 ATTAAT AT^TAAT PstI 137 CTGCAG CTGCA^G SacI 1271 GAGCTC GAGCT^C SacII 23 CCGCGG CCGC^GG SphI 1486 GCATGC GCATG^C SspBI 117 TGTACA T^GTACA TaqI 2092 TCGA T^CGA TthHB8I 2092 TCGA T^CGA Van91I 1303 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 847 TCTAGA T^CTAGA XhoII 964 AGATCT [GA]^GATC[CT] XmnI 805 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BanI 85 GGCACC G^G[CT][GA]CC 529 GGCACC BbsI 1207 GAAGAC GAAGAC(2/6) 1289 GAAGAC BfaI 848 CTAG C^TAG 856 CTAG BpuAI 1207 GAAGAC GAAGAC(2/6) 1289 GAAGAC BsgI 2492 CTGCAC GTGCAG(16/14) 2159 GTGCAG BsmFI 95 GGGAC GGGAC(10/14) 1899 GGGAC EcoRV 418 GATATC GAT^ATC 598 GATATC EcoT14I 450 CCATGG C^C[AT][AT]GG 1186 CCAAGG FauI 9 CCCGC CCCGC(4/6) 45 CCCGC HphI 1345 GGTGA GGTGA(8/7) 2034 GGTGA MaeI 848 CTAG C^TAG 856 CTAG MaeII 98 ACGT A^CGT 1155 ACGT MwoI 39 GCGGAACCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 499 GCTACCATTGC NspI 1486 GCATGC [GA]CATG^[CT] 1755 ACATGC SfcI 137 CTGCAG C^T[GA][CT]AG 2321 CTGTAG SspI 195 AATATT AAT^ATT 433 AATATT StyI 450 CCATGG C^C[AT][AT]GG 1186 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI