Restriction map for
pF1KB8476
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# NO CUT ENZYMES ( 123 enzymes) #
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Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmBI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI FbaI FseI
FspI HaeII HgaI HhaI HinP1I
HincII HindII HpaI KasI KpnI
MluI MluNI MscI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
StuI SwaI Tsp45I Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AatII 97 GACGTC GACGT^C
AccII 24 CGCG CG^CG
AccIII 1218 TCCGGA T^CCGGA
AcyI 97 GACGTC G[GA]^CG[CT]C
AocI 33 CCTGAGG CC^T[ACGT]AGG
AseI 1525 ATTAAT AT^TAAT
AsnI 1525 ATTAAT AT^TAAT
Asp700I 805 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1271 GAGCTC G[AT]GC[AT]^C
AvaII 328 GGACC G^G[AT]CC
BciVI 1544 GGATAC GTATCC(6/5)
BglII 964 AGATCT A^GATCT
BpmI 164 CTGGAG CTGGAG(16/14)
BsaBI 1076 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 97 GACGTC G[GA]^CG[CT]C
BsaI 841 GGTCTC GGTCTC(1/5)
BsaWI 1218 TCCGGA [AT]^CCGG[AT]
BseAI 1218 TCCGGA T^CCGGA
BseRI 1037 CTCCTC GAGGAG(10/8)
BsiHKAI 1271 GAGCTC G[AT]GC[AT]^C
BsmI 978 GAATGC GAATGC(1/-1)
Bsp1407I 117 TGTACA T^GTACA
BspEI 1218 TCCGGA T^CCGGA
BsrDI 504 CATTGC GCAATG(2/0)
BsrGI 117 TGTACA T^GTACA
BssSI 1061 CACGAG CACGAG(-5/-1)
BstEII 2397 GGTTACC G^GT[ACGT]ACC
BstPI 2397 GGTTACC G^GT[ACGT]ACC
BstUI 24 CGCG CG^CG
BstXI 318 CCATGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 964 AGATCT [GA]^GATC[CT]
Bsu36I 33 CCTGAGG CC^T[ACGT]AGG
DraII 5 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 2449 GACAGGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 679 CTCTTC CTCTTC(1/4)
Eco81I 33 CCTGAGG CC^T[ACGT]AGG
EcoO109I 5 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2397 GGTTACC G^GT[ACGT]ACC
EcoT22I 409 ATGCAT ATGCA^T
HapII 1219 CCGG C^CGG
Hin1I 97 GACGTC G[GA]^CG[CT]C
HindIII 2379 AAGCTT A^AGCTT
HpaII 1219 CCGG C^CGG
Ksp632I 679 CTCTTC CTCTTC(1/4)
KspI 23 CCGCGG CCGC^GG
MamI 1076 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 623 CAATTG C^AATTG
MflI 964 AGATCT [GA]^GATC[CT]
MroI 1218 TCCGGA T^CCGGA
MspI 1219 CCGG C^CGG
MunI 623 CAATTG C^AATTG
MvnI 24 CGCG CG^CG
NcoI 450 CCATGG C^CATGG
NsiI 409 ATGCAT ATGCA^T
PflMI 1303 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 816 GACTC GAGTC(4/5)
PshBI 1525 ATTAAT AT^TAAT
PstI 137 CTGCAG CTGCA^G
SacI 1271 GAGCTC GAGCT^C
SacII 23 CCGCGG CCGC^GG
SphI 1486 GCATGC GCATG^C
SspBI 117 TGTACA T^GTACA
TaqI 2092 TCGA T^CGA
TthHB8I 2092 TCGA T^CGA
Van91I 1303 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 847 TCTAGA T^CTAGA
XhoII 964 AGATCT [GA]^GATC[CT]
XmnI 805 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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BanI 85 GGCACC G^G[CT][GA]CC
529 GGCACC
BbsI 1207 GAAGAC GAAGAC(2/6)
1289 GAAGAC
BfaI 848 CTAG C^TAG
856 CTAG
BpuAI 1207 GAAGAC GAAGAC(2/6)
1289 GAAGAC
BsgI 2492 CTGCAC GTGCAG(16/14)
2159 GTGCAG
BsmFI 95 GGGAC GGGAC(10/14)
1899 GGGAC
EcoRV 418 GATATC GAT^ATC
598 GATATC
EcoT14I 450 CCATGG C^C[AT][AT]GG
1186 CCAAGG
FauI 9 CCCGC CCCGC(4/6)
45 CCCGC
HphI 1345 GGTGA GGTGA(8/7)
2034 GGTGA
MaeI 848 CTAG C^TAG
856 CTAG
MaeII 98 ACGT A^CGT
1155 ACGT
MwoI 39 GCGGAACCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
499 GCTACCATTGC
NspI 1486 GCATGC [GA]CATG^[CT]
1755 ACATGC
SfcI 137 CTGCAG C^T[GA][CT]AG
2321 CTGTAG
SspI 195 AATATT AAT^ATT
433 AATATT
StyI 450 CCATGG C^C[AT][AT]GG
1186 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI