Restriction map for
pF1KB9957
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccII AflII AflIII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaJI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspLU11I BsrBI
BsrGI BssHII BssSI BstEII BstPI
BstUI Bsu36I Cac8I CelII ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HpaI KspI MamI MluI MluNI
MscI MspA1I MvnI NaeI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1257 GGTACC G^GTACC
AccIII 443 TCCGGA T^CCGGA
AclI 1106 AACGTT AA^CGTT
AcyI 1028 GGCGCC G[GA]^CG[CT]C
AgeI 101 ACCGGT A^CCGGT
AlwI 1317 GATCC GGATC(4/5)
Asp700I 727 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1257 GGTACC G^GTACC
BbeI 1028 GGCGCC GGCGC^C
BsaHI 1028 GGCGCC G[GA]^CG[CT]C
BseAI 443 TCCGGA T^CCGGA
BsmFI 130 GGGAC GGGAC(10/14)
BsmI 359 GCATTC GAATGC(1/-1)
BspEI 443 TCCGGA T^CCGGA
BspHI 627 TCATGA T^CATGA
BsrFI 101 ACCGGT [GA]^CCGG[CT]
BssKI 886 CCAGG ^CC[ACGT]GG
BstAPI 1054 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1589 TTCGAA TT^CGAA
BstNI 886 CCAGG CC^[AT]GG
BstXI 1348 CCAATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 101 ACCGGT [GA]^CCGG[CT]
DraI 334 TTTAAA TTT^AAA
EarI 1343 CTCTTC CTCTTC(1/4)
EcoNI 441 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 886 CCAGG ^CC[AT]GG
HaeII 1028 GGCGCC [GA]GCGC^[CT]
Hin1I 1028 GGCGCC G[GA]^CG[CT]C
HindIII 176 AAGCTT A^AGCTT
HphI 1517 TCACC GGTGA(8/7)
KasI 1028 GGCGCC G^GCGCC
KpnI 1257 GGTACC GGTAC^C
Ksp632I 1343 CTCTTC CTCTTC(1/4)
MaeII 1107 ACGT A^CGT
MfeI 587 CAATTG C^AATTG
MroI 443 TCCGGA T^CCGGA
MslI 81 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 587 CAATTG C^AATTG
MvaI 886 CCAGG CC^[AT]GG
NarI 1028 GGCGCC GG^CGCC
NspV 1589 TTCGAA TT^CGAA
PflMI 1321 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 101 ACCGGT A^CCGGT
PleI 1311 GAGTC GAGTC(4/5)
Psp1406I 1106 AACGTT AA^CGTT
PstI 1187 CTGCAG CTGCA^G
RcaI 627 TCATGA T^CATGA
SacI 1574 GAGCTC GAGCT^C
ScaI 876 AGTACT AGT^ACT
ScrFI 886 CCAGG CC^[ACGT]GG
SfaNI 1060 GATGC GCATC(5/9)
SfoI 1028 GGCGCC GGC^GCC
SfuI 1589 TTCGAA TT^CGAA
Van91I 1321 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 727 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 953 GTATAC GT^[AC][GT]AC
1163 GTATAC
AlwNI 25 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1181 CAGTTGCTG
AspHI 303 GTGCTC G[AT]GC[AT]^C
1574 GAGCTC
BanI 1028 GGCGCC G^G[CT][GA]CC
1257 GGTACC
BanII 1358 GGGCTC G[GA]GC[CT]^C
1574 GAGCTC
BbsI 1365 GAAGAC GAAGAC(2/6)
1387 GTCTTC
BglII 399 AGATCT A^GATCT
540 AGATCT
BpuAI 1365 GAAGAC GAAGAC(2/6)
1387 GTCTTC
BsaWI 101 ACCGGT [AT]^CCGG[AT]
443 TCCGGA
BsiHKAI 303 GTGCTC G[AT]GC[AT]^C
1574 GAGCTC
BspMI 1377 ACCTGC ACCTGC(4/8)
1324 GCAGGT
BsrDI 1521 CATTGC GCAATG(2/0)
1192 GCAATG
Bst1107I 953 GTATAC GTA^TAC
1163 GTATAC
BstZ17I 953 GTATAC GTA^TAC
1163 GTATAC
CfoI 708 GCGC GCG^C
1029 GCGC
Cfr13I 159 GGGCC G^G[ACGT]CC
486 GGCCC
HaeIII 160 GGCC GG^CC
486 GGCC
HapII 102 CCGG C^CGG
444 CCGG
HhaI 708 GCGC GCG^C
1029 GCGC
HinP1I 708 GCGC G^CGC
1029 GCGC
HpaII 102 CCGG C^CGG
444 CCGG
MspI 102 CCGG C^CGG
444 CCGG
MwoI 331 GCATTTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1054 GCATTGGATGC
NlaIV 1028 GGCGCC GG[ACGT]^[ACGT]CC
1257 GGTACC
NspI 425 GCATGT [GA]CATG^[CT]
1544 GCATGT
Sau96I 159 GGGCC G^G[ACGT]CC
486 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BmyI BpmI BsaI
BsiYI BslI BsmAI Bsp1286I BsrI
BstF5I BstYI DdeI DpnII Fnu4HI
FokI HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
NdeII NlaIII RsaI Sau3AI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII