################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbvCI BbvI BciVI BclI BfaI BglII BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII HpaI ItaI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TseI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 795 CCGC CCGC(-3/-1) AcsI 456 AAATTT [GA]^AATT[CT] AflII 432 CTTAAG C^TTAAG ApoI 456 AAATTT [GA]^AATT[CT] BbrPI 276 CACGTG CAC^GTG BcgI 821 GCAATCCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 789 CCGGG CC^[GC]GG BfrI 432 CTTAAG C^TTAAG BglI 812 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 814 CTGGAG CTGGAG(16/14) BsiYI 651 CCTCTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 651 CCTCTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 791 GGGAC GGGAC(10/14) BsmI 622 GCATTC GAATGC(1/-1) Bst1107I 310 GTATAC GTA^TAC BstZ17I 310 GTATAC GTA^TAC Cac8I 54 GCCAGC GC[ACGT]^[ACGT]GC DrdI 6 GACTCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 802 GAAGAG CTCTTC(1/4) EcoT22I 620 ATGCAT ATGCA^T HapII 789 CCGG C^CGG HincII 325 GTTGAC GT[CT]^[GA]AC HindII 325 GTTGAC GT[CT]^[GA]AC HpaII 789 CCGG C^CGG HphI 644 TCACC GGTGA(8/7) Ksp632I 802 GAAGAG CTCTTC(1/4) MspI 789 CCGG C^CGG MwoI 812 GCCTGGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 789 CCGGG CC^[GC]GG NlaIV 791 GGGACC GG[ACGT]^[ACGT]CC NsiI 620 ATGCAT ATGCA^T PmaCI 276 CACGTG CAC^GTG PmlI 276 CACGTG CAC^GTG PstI 10 CTGCAG CTGCA^G SfcI 10 CTGCAG C^T[GA][CT]AG SmlI 432 CTTAAG C^T[CT][GA]AG SspI 166 AATATT AAT^ATT TaqI 443 TCGA T^CGA TthHB8I 443 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 340 GTAC GT^AC 482 GTAC AflIII 277 ACGTGT A^C[GA][CT]GT 708 ACGTGT AvaII 660 GGACC G^G[AT]CC 792 GGACC BsaAI 276 CACGTG [CT]AC^GT[GA] 707 TACGTG BsaJI 671 CCCTGG C^C[ACGT][ACGT]GG 834 CCTGGG BsrI 547 CCAGT ACTGG(1/-1) 779 CCAGT Cfr13I 660 GGACC G^G[ACGT]CC 792 GGACC MaeII 277 ACGT A^CGT 708 ACGT MaeIII 30 GTTAC ^GT[ACGT]AC 782 GTTAC RsaI 340 GTAC GT^AC 482 GTAC Sau96I 660 GGACC G^G[ACGT]CC 792 GGACC SfaNI 334 GATGC GCATC(5/9) 843 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AccI AluI BbsI BpuAI BssKI BstF5I BstNI EcoRII FokI HinfI MboII MnlI MseI MvaI PleI ScrFI TfiI Tru9I Tsp509I