Restriction map for
pF1KB6678
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BanI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspEI
BspHI BspMI BsrGI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CpoI DraI DraII DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HaeII HindIII HpaI KasI KpnI
Ksp632I KspI MluI MluNI MroI
MscI MspA1I NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflIII 1001 ACATGT A^C[GA][CT]GT
ApaI 45 GGGCCC GGGCC^C
Asp700I 177 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 507 GGATCC G^GATCC
BbsI 350 GTCTTC GAAGAC(2/6)
BbvCI 385 GCTGAGG CCTCAGC(-5/-2)
BglI 28 GCCGCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 99 CTCCAG CTGGAG(16/14)
BpuAI 350 GTCTTC GAAGAC(2/6)
BsaBI 426 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 956 GAGGAG GAGGAG(10/8)
BsgI 1013 CTGCAC GTGCAG(16/14)
BspDI 545 ATCGAT AT^CGAT
BspLU11I 1001 ACATGT A^CATGT
BsrBI 166 CCGCTC CCGCTC(-3/-3)
BstAPI 240 GCAAGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 507 GGATCC [GA]^GATC[CT]
ClaI 545 ATCGAT AT^CGAT
DraIII 823 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1255 GACTGGTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 35 TGGCCG [CT]^GGCC[GA]
FseI 36 GGCCGGCC GGCCGG^CC
HincII 1176 GTCAAC GT[CT]^[GA]AC
HindII 1176 GTCAAC GT[CT]^[GA]AC
MamI 426 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 322 CAATTG C^AATTG
MflI 507 GGATCC [GA]^GATC[CT]
MslI 445 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 322 CAATTG C^AATTG
NspI 1001 ACATGT [GA]CATG^[CT]
ScaI 533 AGTACT AGT^ACT
TfiI 548 GATTC G^A[AT]TC
XhoII 507 GGATCC [GA]^GATC[CT]
XmnI 177 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 108 GGCGTC G[GA]^CG[CT]C
710 GGCGTC
AlwNI 181 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
416 CAGATACTG
BanII 45 GGGCCC G[GA]GC[CT]^C
96 GGGCTC
BmyI 45 GGGCCC G[AGT]GC[ACT]^C
96 GGGCTC
BsaHI 108 GGCGTC G[GA]^CG[CT]C
710 GGCGTC
BsaI 1033 GAGACC GGTCTC(1/5)
1112 GAGACC
BsmI 204 GAATGC GAATGC(1/-1)
846 GAATGC
Bsp1286I 45 GGGCCC G[AGT]GC[ACT]^C
96 GGGCTC
BsrDI 668 GCAATG GCAATG(2/0)
850 GCAATG
BsrFI 37 GCCGGC [GA]^CCGG[CT]
64 GCCGGC
BssHII 57 GCGCGC G^CGCGC
148 GCGCGC
BssSI 1243 CACGAG CACGAG(-5/-1)
1025 CTCGTG
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
64 GCCGGC
DdeI 304 CTCAG C^T[ACGT]AG
386 CTGAG
EcoT14I 32 CCTTGG C^C[AT][AT]GG
1107 CCAAGG
FauI 165 CCCGC CCCGC(4/6)
1040 CCCGC
Hin1I 108 GGCGTC G[GA]^CG[CT]C
710 GGCGTC
HphI 515 TCACC GGTGA(8/7)
775 TCACC
MaeII 1086 ACGT A^CGT
1294 ACGT
NaeI 37 GCCGGC GCC^GGC
64 GCCGGC
NgoMIV 37 GCCGGC G^CCGGC
64 GCCGGC
SfaNI 391 GATGC GCATC(5/9)
1150 GATGC
SmaI 42 CCCGGG CCC^GGG
48 CCCGGG
StyI 32 CCTTGG C^C[AT][AT]GG
1107 CCAAGG
TaqI 546 TCGA T^CGA
636 TCGA
TthHB8I 546 TCGA T^CGA
636 TCGA
XmaCI 42 CCCGGG C^CCGGG
48 CCCGGG
XmaI 42 CCCGGG C^CCGGG
48 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI BbvI BcnI BfaI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI Tru9I
TseI Tsp45I Tsp509I TspRI