################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 258 GTCTAC GT^[AC][GT]AC AccII 1026 CGCG CG^CG AciI 1025 CCGC CCGC(-3/-1) AgeI 58 ACCGGT A^CCGGT AlwNI 1223 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 240 ATTAAT AT^TAAT AsnI 240 ATTAAT AT^TAAT Asp700I 967 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 329 GTGCTC G[AT]GC[AT]^C AvrII 936 CCTAGG C^CTAGG BbsI 318 GTCTTC GAAGAC(2/6) BbvI 164 GCAGC GCAGC(8/12) BlnI 936 CCTAGG C^CTAGG BmyI 329 GTGCTC G[AGT]GC[ACT]^C BpuAI 318 GTCTTC GAAGAC(2/6) BsaWI 58 ACCGGT [AT]^CCGG[AT] BseRI 1184 GAGGAG GAGGAG(10/8) BsiHKAI 329 GTGCTC G[AT]GC[AT]^C BsmBI 765 GAGACG CGTCTC(1/5) Bsp1286I 329 GTGCTC G[AGT]GC[ACT]^C BspMI 21 ACCTGC ACCTGC(4/8) BsrDI 828 CATTGC GCAATG(2/0) BsrFI 58 ACCGGT [GA]^CCGG[CT] BstBI 500 TTCGAA TT^CGAA BstUI 1026 CGCG CG^CG BstXI 865 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 657 AGATCC [GA]^GATC[CT] Cac8I 448 GCTTGC GC[ACGT]^[ACGT]GC Cfr10I 58 ACCGGT [GA]^CCGG[CT] EcoT14I 936 CCTAGG C^C[AT][AT]GG Fnu4HI 164 GCAGC GC^[ACGT]GC HaeIII 981 GGCC GG^CC HapII 59 CCGG C^CGG HpaII 59 CCGG C^CGG HphI 862 TCACC GGTGA(8/7) ItaI 164 GCAGC GC^[ACGT]GC MaeII 210 ACGT A^CGT MflI 657 AGATCC [GA]^GATC[CT] MslI 1080 CACTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1317 CAGCTG C[AC]G^C[GT]G MspI 59 CCGG C^CGG MvnI 1026 CGCG CG^CG NdeI 976 CATATG CA^TATG NspI 737 ACATGC [GA]CATG^[CT] NspV 500 TTCGAA TT^CGAA PinAI 58 ACCGGT A^CCGGT PleI 1187 GAGTC GAGTC(4/5) PshBI 240 ATTAAT AT^TAAT PvuII 1317 CAGCTG CAG^CTG SfuI 500 TTCGAA TT^CGAA SpeI 928 ACTAGT A^CTAGT SspI 136 AATATT AAT^ATT StyI 936 CCTAGG C^C[AT][AT]GG TseI 164 GCAGC G^C[AT]GC XhoII 657 AGATCC [GA]^GATC[CT] XmnI 967 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 1144 GGTACC G^GTACC 1238 GGTACC AcsI 301 AAATTC [GA]^AATT[CT] 1214 GAATTT AlwI 658 GATCC GGATC(4/5) 898 GGATC ApoI 301 AAATTC [GA]^AATT[CT] 1214 GAATTT Asp718I 1144 GGTACC G^GTACC 1238 GGTACC AvaII 61 GGTCC G^G[AT]CC 1106 GGTCC BclI 285 TGATCA T^GATCA 468 TGATCA BsmAI 765 GAGAC GTCTC(1/5) 615 GTCTC BsmI 750 GAATGC GAATGC(1/-1) 178 GCATTC Cfr13I 61 GGTCC G^G[ACGT]CC 1106 GGTCC FbaI 285 TGATCA T^GATCA 468 TGATCA KpnI 1144 GGTACC GGTAC^C 1238 GGTACC PflMI 341 CCAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 434 CCACATTTTGG PstI 220 CTGCAG CTGCA^G 1193 CTGCAG Sau96I 61 GGTCC G^G[ACGT]CC 1106 GGTCC SfaNI 1369 GATGC GCATC(5/9) 397 GCATC SfcI 220 CTGCAG C^T[GA][CT]AG 1193 CTGCAG TaqI 501 TCGA T^CGA 1132 TCGA TfiI 859 GATTC G^A[AT]TC 1357 GAATC Tsp45I 207 GTCAC ^GT[GC]AC 1189 GTCAC TthHB8I 501 TCGA T^CGA 1132 TCGA Van91I 341 CCAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 434 CCACATTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AluI BanI BfaI BsaJI BsiYI BslI BsrI BssKI BstNI DdeI DpnII EcoRII HinfI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI Tru9I Tsp509I TspRI