Restriction map for
pF1KE0528
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmFI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DpnII DraI DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HapII Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 229 GTCTAC GT^[AC][GT]AC
Alw44I 70 GTGCAC G^TGCAC
Aor51HI 335 AGCGCT AGC^GCT
ApaI 292 GGGCCC GGGCC^C
ApaLI 70 GTGCAC G^TGCAC
BanI 696 GGCACC G^G[CT][GA]CC
BbsI 217 GTCTTC GAAGAC(2/6)
BglI 294 GCCCCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 217 GTCTTC GAAGAC(2/6)
BsgI 247 CTGCAC GTGCAG(16/14)
BsmBI 131 GAGACG CGTCTC(1/5)
BsmI 87 GAATGC GAATGC(1/-1)
BspHI 98 TCATGA T^CATGA
BspMI 467 ACCTGC ACCTGC(4/8)
BsrDI 513 CATTGC GCAATG(2/0)
BssSI 355 CACGAG CACGAG(-5/-1)
DraIII 355 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 746 CCGTGG C^C[GA][CT]GG
Eco47III 335 AGCGCT AGC^GCT
HaeII 335 AGCGCT [GA]GCGC^[CT]
HgaI 6 GCGTC GACGC(5/10)
NspI 23 GCATGC [GA]CATG^[CT]
PflMI 354 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 98 TCATGA T^CATGA
SapI 241 GAAGAGC GCTCTTC(1/4)
SfcI 845 CTGTAG C^T[GA][CT]AG
SphI 23 GCATGC GCATG^C
TfiI 736 GATTC G^A[AT]TC
TspRI 519 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 354 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 162 GTAC GT^AC
288 GTAC
AspHI 70 GTGCAC G[AT]GC[AT]^C
436 GTGCTC
AvaI 128 CCCGAG C^[CT]CG[GA]G
376 CCCGAG
BsiHKAI 70 GTGCAC G[AT]GC[AT]^C
436 GTGCTC
BsmAI 131 GAGAC GTCTC(1/5)
751 GAGAC
BsoBI 128 CCCGAG C^[CT]CG[GA]G
376 CCCGAG
BsrBI 546 CCGCTC CCGCTC(-3/-3)
783 CCGCTC
BsrI 214 CCAGT ACTGG(1/-1)
518 CCAGT
BstAPI 82 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
651 GCAGGAGCTGC
BstEII 123 GGTGACC G^GT[ACGT]ACC
415 GGTGACC
BstF5I 312 CATCC GGATG(2/0)
630 CATCC
BstPI 123 GGTGACC G^GT[ACGT]ACC
415 GGTGACC
DdeI 92 CTGAG C^T[ACGT]AG
820 CTCAG
EarI 241 GAAGAG CTCTTC(1/4)
601 GAAGAG
EcoO65I 123 GGTGACC G^GT[ACGT]ACC
415 GGTGACC
EcoT14I 62 CCAAGG C^C[AT][AT]GG
674 CCAAGG
FokI 312 CATCC GGATG(9/13)
630 CATCC
Ksp632I 241 GAAGAG CTCTTC(1/4)
601 GAAGAG
MaeIII 124 GTGAC ^GT[ACGT]AC
416 GTGAC
MslI 166 CACATCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
168 CATCTATGTG
MspA1I 297 CCGCTG C[AC]G^C[GT]G
712 CAGCGG
PleI 573 GACTC GAGTC(4/5)
753 GACTC
PpuMI 272 AGGACCT [GA]G^G[AT]CC[CT]
809 GGGTCCT
RsaI 162 GTAC GT^AC
288 GTAC
StyI 62 CCAAGG C^C[AT][AT]GG
674 CCAAGG
Tsp45I 124 GTGAC ^GT[GC]AC
416 GTGAC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
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AciI AluI AlwNI AvaII BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I CfoI Cfr13I DraII EcoNI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HhaI HinP1I HinfI HphI ItaI
MboII MnlI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TseI