Restriction map for
pF1KE1065
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbvCI BciVI BfaI BfrI
BlnI BlpI Bpu1102I BseAI BsiWI
Bsp1407I BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI MaeI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshBI Psp1406I PvuI RsrII SapI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 94 GTCGAC GT^[AC][GT]AC
AcsI 2804 AAATTT [GA]^AATT[CT]
Alw44I 2221 GTGCAC G^TGCAC
ApaLI 2221 GTGCAC G^TGCAC
ApoI 2804 AAATTT [GA]^AATT[CT]
Asp700I 2862 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2304 TGGCCA TGG^CCA
BclI 2825 TGATCA T^GATCA
BsaBI 2505 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 758 ACCGGA [AT]^CCGG[AT]
BseRI 1587 CTCCTC GAGGAG(10/8)
BsmI 2467 GAATGC GAATGC(1/-1)
BspDI 136 ATCGAT AT^CGAT
BspHI 2567 TCATGA T^CATGA
ClaI 136 ATCGAT AT^CGAT
DraIII 1989 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2621 CCTACGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2825 TGATCA T^GATCA
KspI 1047 CCGCGG CCGC^GG
MamI 2505 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2304 TGGCCA TGG^CCA
MscI 2304 TGGCCA TGG^CCA
NaeI 1769 GCCGGC GCC^GGC
NgoMIV 1769 GCCGGC G^CCGGC
PflMI 1962 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 355 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2567 TCATGA T^CATGA
SacI 1924 GAGCTC GAGCT^C
SacII 1047 CCGCGG CCGC^GG
SalI 94 GTCGAC G^TCGAC
SbfI 1731 CCTGCAGG CCTGCA^GG
SmlI 1576 CTCAAG C^T[CT][GA]AG
Sse8387I 1731 CCTGCAGG CCTGCA^GG
Van91I 1962 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2862 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 673 ACGTGT A^C[GA][CT]GT
2036 ACGTGT
ApaI 1247 GGGCCC GGGCC^C
1417 GGGCCC
AviII 733 TGCGCA TGC^GCA
1754 TGCGCA
BamHI 2504 GGATCC G^GATCC
2666 GGATCC
BbeI 1601 GGCGCC GGCGC^C
2774 GGCGCC
BbrPI 672 CACGTG CAC^GTG
717 CACGTG
BbsI 217 GAAGAC GAAGAC(2/6)
831 GAAGAC
BglII 389 AGATCT A^GATCT
2525 AGATCT
BpmI 2063 CTCCAG CTGGAG(16/14)
1837 CTGGAG
BpuAI 217 GAAGAC GAAGAC(2/6)
831 GAAGAC
BsgI 603 CTGCAC GTGCAG(16/14)
1095 GTGCAG
BsmBI 535 GAGACG CGTCTC(1/5)
727 GAGACG
BssSI 265 CACGAG CACGAG(-5/-1)
1514 CACGAG
BstXI 1829 CCAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1911 CCACTTCGGTGG
DraII 666 AGGCCCC [GA]G^G[ACGT]CC[CT]
2831 AGGCCCT
EcoO109I 666 AGGCCCC [GA]G^G[ACGT]CC[CT]
2831 AGGCCCT
FspI 733 TGCGCA TGC^GCA
1754 TGCGCA
KasI 1601 GGCGCC G^GCGCC
2774 GGCGCC
NarI 1601 GGCGCC GG^CGCC
2774 GGCGCC
NcoI 404 CCATGG C^CATGG
510 CCATGG
PmaCI 672 CACGTG CAC^GTG
717 CACGTG
PmlI 672 CACGTG CAC^GTG
717 CACGTG
SfoI 1601 GGCGCC GGC^GCC
2774 GGCGCC
StuI 1679 AGGCCT AGG^CCT
2102 AGGCCT
TfiI 809 GATTC G^A[AT]TC
2887 GAATC
Tsp509I 619 AATT ^AATT
2805 AATT
XcmI 542 CCAAAGGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2292 CCAGCCCGAGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcgI BcnI
BglI BmyI BsaAI BsaHI BsaI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EagI EarI EclXI
Eco52I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI