Restriction map for
pF1KB4458
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
Bsp1407I BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
CpoI EcoRV FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NcoI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 2827 GTCTAC GT^[AC][GT]AC
AhdI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2500 GTGCAC G^TGCAC
ApaLI 2500 GTGCAC G^TGCAC
AspEI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2800 CCTCAGC CCTCAGC(-5/-2)
BcgI 3453 CGACAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1736 GTATCC GTATCC(6/5)
BfaI 1267 CTAG C^TAG
BglII 2765 AGATCT A^GATCT
BsiEI 17 CGGCCG CG[GA][CT]^CG
BspDI 2821 ATCGAT AT^CGAT
BspLU11I 524 ACATGT A^CATGT
BsrDI 270 CATTGC GCAATG(2/0)
BstEII 2703 GGTGACC G^GT[ACGT]ACC
BstPI 2703 GGTGACC G^GT[ACGT]ACC
ClaI 2821 ATCGAT AT^CGAT
DraI 1033 TTTAAA TTT^AAA
DraIII 2078 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 739 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 17 CGGCCG C^GGCCG
Eam1105I 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 17 CGGCCG C^GGCCG
Eco52I 17 CGGCCG C^GGCCG
EcoO65I 2703 GGTGACC G^GT[ACGT]ACC
EcoT22I 2959 ATGCAT ATGCA^T
HincII 3439 GTCAAC GT[CT]^[GA]AC
HindII 3439 GTCAAC GT[CT]^[GA]AC
MaeI 1267 CTAG C^TAG
NaeI 1837 GCCGGC GCC^GGC
NgoMIV 1837 GCCGGC G^CCGGC
NsiI 2959 ATGCAT ATGCA^T
SacI 3105 GAGCTC GAGCT^C
SmaI 728 CCCGGG CCC^GGG
XmaCI 728 CCCGGG C^CCGGG
XmaI 728 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 524 ACATGT A^C[GA][CT]GT
2318 ACGTGT
AocI 1690 CCTCAGG CC^T[ACGT]AGG
3591 CCTAAGG
Aor51HI 27 AGCGCT AGC^GCT
2927 AGCGCT
BalI 2297 TGGCCA TGG^CCA
2573 TGGCCA
BbrPI 774 CACGTG CAC^GTG
2317 CACGTG
BclI 2834 TGATCA T^GATCA
2891 TGATCA
BsaI 2918 GAGACC GGTCTC(1/5)
3362 GAGACC
BsmBI 1569 CGTCTC CGTCTC(1/5)
764 GAGACG
BsmI 1600 GAATGC GAATGC(1/-1)
2957 GAATGC
BsrFI 1116 GCCGGT [GA]^CCGG[CT]
1837 GCCGGC
BssSI 99 CACGAG CACGAG(-5/-1)
915 CTCGTG
BstXI 1940 CCACTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2576 CCAAACTGCTGG
Bsu36I 1690 CCTCAGG CC^T[ACGT]AGG
3591 CCTAAGG
Cfr10I 1116 GCCGGT [GA]^CCGG[CT]
1837 GCCGGC
DsaI 669 CCGTGG C^C[GA][CT]GG
3137 CCGTGG
EarI 1996 CTCTTC CTCTTC(1/4)
2593 GAAGAG
Eco47III 27 AGCGCT AGC^GCT
2927 AGCGCT
Eco81I 1690 CCTCAGG CC^T[ACGT]AGG
3591 CCTAAGG
EcoNI 1620 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1686 CCTGCCTCAGG
FbaI 2834 TGATCA T^GATCA
2891 TGATCA
Ksp632I 1996 CTCTTC CTCTTC(1/4)
2593 GAAGAG
MluNI 2297 TGGCCA TGG^CCA
2573 TGGCCA
MscI 2297 TGGCCA TGG^CCA
2573 TGGCCA
NdeI 2741 CATATG CA^TATG
2811 CATATG
NspI 524 ACATGT [GA]CATG^[CT]
2547 GCATGT
PflMI 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1909 CCAACGAATGG
PmaCI 774 CACGTG CAC^GTG
2317 CACGTG
PmlI 774 CACGTG CAC^GTG
2317 CACGTG
PpuMI 1444 GGGACCT [GA]G^G[AT]CC[CT]
3377 AGGACCC
SmlI 1357 CTCAAG C^T[CT][GA]AG
2109 CTTGAG
SspI 1063 AATATT AAT^ATT
2422 AATATT
Van91I 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1909 CCAACGAATGG
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BamHI
BanI BanII BbeI BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflFI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII