################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 159 CCGC CCGC(-3/-1) AcsI 23 AAATTC [GA]^AATT[CT] AfaI 68 GTAC GT^AC AhdI 366 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 23 AAATTC [GA]^AATT[CT] Asp700I 19 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 366 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 35 GAGCTC G[AT]GC[AT]^C AvaII 177 GGACC G^G[AT]CC BanII 35 GAGCTC G[GA]GC[CT]^C BbsI 210 GAAGAC GAAGAC(2/6) BbvI 400 GCAGC GCAGC(8/12) BcnI 360 CCCGG CC^[GC]GG BmyI 35 GAGCTC G[AGT]GC[ACT]^C BpuAI 210 GAAGAC GAAGAC(2/6) BseRI 72 GAGGAG GAGGAG(10/8) BsiHKAI 35 GAGCTC G[AT]GC[AT]^C BsiYI 388 CCAACAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 388 CCAACAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 35 GAGCTC G[AGT]GC[ACT]^C BsrI 404 CCAGT ACTGG(1/-1) DdeI 101 CTCAG C^T[ACGT]AG DpnII 586 GATC ^GATC Eam1105I 366 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 457 GAAGAG CTCTTC(1/4) Fnu4HI 400 GCAGC GC^[ACGT]GC HapII 361 CCGG C^CGG HpaII 361 CCGG C^CGG ItaI 400 GCAGC GC^[ACGT]GC Ksp632I 457 GAAGAG CTCTTC(1/4) MaeIII 364 GTGAC ^GT[ACGT]AC MboI 586 GATC ^GATC MnlI 72 GAGG CCTC(7/6) MslI 123 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 361 CCGG C^CGG NciI 360 CCCGG CC^[GC]GG NdeII 586 GATC ^GATC RsaI 68 GTAC GT^AC SacI 35 GAGCTC GAGCT^C Sau3AI 586 GATC ^GATC SmlI 470 CTCAAG C^T[CT][GA]AG TseI 400 GCAGC G^C[AT]GC Tsp45I 364 GTGAC ^GT[GC]AC Tsp509I 24 AATT ^AATT TspRI 405 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 122 CCATCACTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 19 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AluI 36 AGCT AG^CT 377 AGCT BciVI 412 GGATAC GTATCC(6/5) 482 GTATCC BsaJI 81 CCTGGG C^C[ACGT][ACGT]GG 486 CCTGGG BsrDI 335 GCAATG GCAATG(2/0) 521 GCAATG BstF5I 105 GGATG GGATG(2/0) 135 GGATG FokI 105 GGATG GGATG(9/13) 135 GGATG MseI 217 TTAA T^TAA 419 TTAA Tru9I 217 TTAA T^TAA 419 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### BsmAI BssKI BstNI Cfr13I EcoRII HaeIII HinfI HphI MboII MvaI NlaIII PleI Sau96I ScrFI SfaNI TfiI