Restriction map for
pF1KE1345
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflIII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BglI BglII BlnI BpuAI BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRI EcoRV EcoT22I FauI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MfeI MflI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SwaI Tsp509I Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflII 112 CTTAAG C^TTAAG
AhdI 395 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 395 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 478 GTGCTC G[AT]GC[AT]^C
AvaI 50 CTCGGG C^[CT]CG[GA]G
BalI 737 TGGCCA TGG^CCA
BclI 581 TGATCA T^GATCA
BfrI 112 CTTAAG C^TTAAG
BmyI 478 GTGCTC G[AGT]GC[ACT]^C
BsaBI 177 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 167 GAGACC GGTCTC(1/5)
BsiHKAI 478 GTGCTC G[AT]GC[AT]^C
BsoBI 50 CTCGGG C^[CT]CG[GA]G
Bsp1286I 478 GTGCTC G[AGT]GC[ACT]^C
BstEII 330 GGTGACC G^GT[ACGT]ACC
BstPI 330 GGTGACC G^GT[ACGT]ACC
DraIII 486 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 734 CCATGG C^C[GA][CT]GG
EaeI 737 TGGCCA [CT]^GGCC[GA]
Eam1105I 395 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 524 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 330 GGTGACC G^GT[ACGT]ACC
FbaI 581 TGATCA T^GATCA
HaeII 534 GGCGCT [GA]GCGC^[CT]
MamI 177 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 737 TGGCCA TGG^CCA
MscI 737 TGGCCA TGG^CCA
NcoI 734 CCATGG C^CATGG
PflMI 438 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 270 CCTGCAGG CCTGCA^GG
Sse8387I 270 CCTGCAGG CCTGCA^GG
SspI 68 AATATT AAT^ATT
StuI 308 AGGCCT AGG^CCT
TaqI 695 TCGA T^CGA
TthHB8I 695 TCGA T^CGA
Van91I 438 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 635 CCAGCCTCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 503 CCGC CCGC(-3/-1)
400 GCGG
AvaII 320 GGTCC G^G[AT]CC
370 GGTCC
BcnI 465 CCCGG CC^[GC]GG
651 CCGGG
BlpI 480 GCTCAGC GC^T[ACGT]AGC
777 GCTGAGC
BpmI 633 CTCCAG CTGGAG(16/14)
280 CTGGAG
Bpu1102I 480 GCTCAGC GC^T[ACGT]AGC
777 GCTGAGC
BseRI 211 CTCCTC GAGGAG(10/8)
382 GAGGAG
BsmAI 167 GAGAC GTCTC(1/5)
393 GAGAC
BsmFI 321 GTCCC GGGAC(10/14)
454 GTCCC
BstF5I 498 GGATG GGATG(2/0)
588 GGATG
CelII 480 GCTCAGC GC^T[ACGT]AGC
777 GCTGAGC
CfoI 535 GCGC GCG^C
592 GCGC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
734 CCATGG
FokI 498 GGATG GGATG(9/13)
588 GGATG
HapII 466 CCGG C^CGG
651 CCGG
HhaI 535 GCGC GCG^C
592 GCGC
HinP1I 535 GCGC G^CGC
592 GCGC
HpaII 466 CCGG C^CGG
651 CCGG
MboII 294 GAAGA GAAGA(8/7)
727 TCTTC
MspI 466 CCGG C^CGG
651 CCGG
MwoI 476 GCGTGCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
658 GCAGTAGAGGC
NciI 465 CCCGG CC^[GC]GG
651 CCGGG
PleI 429 GACTC GAGTC(4/5)
362 GAGTC
SfaNI 499 GATGC GCATC(5/9)
589 GATGC
SmlI 112 CTTAAG C^T[CT][GA]AG
595 CTCAAG
StyI 20 CCAAGG C^C[AT][AT]GG
734 CCATGG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwNI BbvI BfaI BsaJI
BsiYI BslI BspMI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MnlI
MseI MvaI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
TfiI Tru9I TseI Tsp45I TspRI