Restriction map for
pF1KB6939
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AhdI Alw44I AlwI AlwNI
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PleI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 417 GACGTC GACGT^C
AflIII 371 ACACGT A^C[GA][CT]GT
AgeI 568 ACCGGT A^CCGGT
ApaI 325 GGGCCC GGGCC^C
Asp700I 147 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 437 GGCACC G^G[CT][GA]CC
BbrPI 372 CACGTG CAC^GTG
BbsI 471 GAAGAC GAAGAC(2/6)
BpmI 339 CTCCAG CTGGAG(16/14)
BpuAI 471 GAAGAC GAAGAC(2/6)
BsaAI 372 CACGTG [CT]AC^GT[GA]
BsaWI 568 ACCGGT [AT]^CCGG[AT]
BseRI 295 GAGGAG GAGGAG(10/8)
BsmAI 420 GTCTC GTCTC(1/5)
BsmBI 419 CGTCTC CGTCTC(1/5)
BspMI 255 GCAGGT ACCTGC(4/8)
BstEII 258 GGTCACC G^GT[ACGT]ACC
BstPI 258 GGTCACC G^GT[ACGT]ACC
DdeI 407 CTGAG C^T[ACGT]AG
DsaI 281 CCGCGG C^C[GA][CT]GG
EaeI 507 CGGCCA [CT]^GGCC[GA]
EarI 544 CTCTTC CTCTTC(1/4)
EcoO65I 258 GGTCACC G^GT[ACGT]ACC
EcoT14I 554 CCAAGG C^C[AT][AT]GG
HinfI 539 GAATC G^A[ACGT]TC
Ksp632I 544 CTCTTC CTCTTC(1/4)
KspI 281 CCGCGG CCGC^GG
MspA1I 281 CCGCGG C[AC]G^C[GT]G
NlaIII 139 CATG CATG^
PinAI 568 ACCGGT A^CCGGT
PmaCI 372 CACGTG CAC^GTG
PmlI 372 CACGTG CAC^GTG
SacI 54 GAGCTC GAGCT^C
SacII 281 CCGCGG CCGC^GG
SgrAI 567 CACCGGTG C[GA]^CCGG[CT]G
SmaI 121 CCCGGG CCC^GGG
SmlI 88 CTCAAG C^T[CT][GA]AG
StyI 554 CCAAGG C^C[AT][AT]GG
TaqI 272 TCGA T^CGA
TfiI 539 GAATC G^A[AT]TC
TthHB8I 272 TCGA T^CGA
XmaCI 121 CCCGGG C^CCGGG
XmaI 121 CCCGGG C^CCGGG
XmnI 147 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 117 GACGCC G[GA]^CG[CT]C
417 GACGTC
AfaI 36 GTAC GT^AC
399 GTAC
AluI 55 AGCT AG^CT
583 AGCT
Aor51HI 98 AGCGCT AGC^GCT
461 AGCGCT
AspHI 54 GAGCTC G[AT]GC[AT]^C
499 GAGCAC
AvaI 121 CCCGGG C^[CT]CG[GA]G
287 CCCGAG
BsaHI 117 GACGCC G[GA]^CG[CT]C
417 GACGTC
BsiHKAI 54 GAGCTC G[AT]GC[AT]^C
499 GAGCAC
BsmFI 599 GGGAC GGGAC(10/14)
277 GTCCC
BsoBI 121 CCCGGG C^[CT]CG[GA]G
287 CCCGAG
BsrBI 298 GAGCGG CCGCTC(-3/-3)
309 GAGCGG
BsrI 71 ACTGG ACTGG(1/-1)
341 CCAGT
BstNI 60 CCTGG CC^[AT]GG
240 CCTGG
Eco47III 98 AGCGCT AGC^GCT
461 AGCGCT
EcoNI 60 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
240 CCTGGCGGAGG
EcoRII 60 CCTGG ^CC[AT]GG
240 CCTGG
HaeII 98 AGCGCT [GA]GCGC^[CT]
461 AGCGCT
HgaI 111 GACGC GACGC(5/10)
117 GACGC
Hin1I 117 GACGCC G[GA]^CG[CT]C
417 GACGTC
MaeII 373 ACGT A^CGT
418 ACGT
MboII 471 GAAGA GAAGA(8/7)
545 TCTTC
MvaI 60 CCTGG CC^[AT]GG
240 CCTGG
PpuMI 275 AGGTCCC [GA]G^G[AT]CC[CT]
599 GGGACCC
PstI 102 CTGCAG CTGCA^G
384 CTGCAG
RsaI 36 GTAC GT^AC
399 GTAC
SfcI 102 CTGCAG C^T[GA][CT]AG
384 CTGCAG
TspRI 342 CAGTG CA[GC]TG[ACGT][ACGT]^
476 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AvaII BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrFI BssKI BstUI Cac8I
CfoI Cfr10I Cfr13I DraII EcoO109I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MnlI MspI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI Tsp45I